################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BbeI BbsI BbvCI BciVI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 341 AAATTC [GA]^AATT[CT] AflIII 129 ACACGT A^C[GA][CT]GT AlwI 1034 GGATC GGATC(4/5) ApaI 269 GGGCCC GGGCC^C ApoI 341 AAATTC [GA]^AATT[CT] Asp700I 1227 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 928 GAGCTC G[AT]GC[AT]^C AvaI 954 CTCGAG C^[CT]CG[GA]G AviII 368 TGCGCA TGC^GCA BanI 720 GGTGCC G^G[CT][GA]CC BbrPI 130 CACGTG CAC^GTG BcgI 489 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 762 CCGGG CC^[GC]GG BglII 579 AGATCT A^GATCT BsaBI 673 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 880 GAGACC GGTCTC(1/5) BsgI 780 CTGCAC GTGCAG(16/14) BsiHKAI 928 GAGCTC G[AT]GC[AT]^C BsoBI 954 CTCGAG C^[CT]CG[GA]G Bsp1407I 179 TGTACA T^GTACA BspHI 1238 TCATGA T^CATGA BspMI 935 ACCTGC ACCTGC(4/8) BsrDI 809 GCAATG GCAATG(2/0) BsrGI 179 TGTACA T^GTACA BstXI 242 CCACCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 579 AGATCT [GA]^GATC[CT] DraI 637 TTTAAA TTT^AAA DsaI 785 CCGTGG C^C[GA][CT]GG EarI 823 CTCTTC CTCTTC(1/4) EcoT14I 248 CCTTGG C^C[AT][AT]GG FspI 368 TGCGCA TGC^GCA HapII 762 CCGG C^CGG HpaII 762 CCGG C^CGG Ksp632I 823 CTCTTC CTCTTC(1/4) MamI 673 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 579 AGATCT [GA]^GATC[CT] MseI 638 TTAA T^TAA MslI 783 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 762 CCGG C^CGG NciI 762 CCGGG CC^[GC]GG PaeR7I 954 CTCGAG C^TCGAG PmaCI 130 CACGTG CAC^GTG PmlI 130 CACGTG CAC^GTG PpuMI 590 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1238 TCATGA T^CATGA SacI 928 GAGCTC GAGCT^C SspBI 179 TGTACA T^GTACA StyI 248 CCTTGG C^C[AT][AT]GG TaqI 955 TCGA T^CGA Tru9I 638 TTAA T^TAA Tsp45I 1249 GTCAC ^GT[GC]AC TthHB8I 955 TCGA T^CGA XhoI 954 CTCGAG C^TCGAG XhoII 579 AGATCT [GA]^GATC[CT] XmnI 1227 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 180 GTAC GT^AC 801 GTAC BfaI 563 CTAG C^TAG 1054 CTAG BpmI 985 CTCCAG CTGGAG(16/14) 907 CTGGAG BsaAI 130 CACGTG [CT]AC^GT[GA] 547 TACGTG BstAPI 360 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1114 GCATCTGCTGC BstF5I 828 CATCC GGATG(2/0) 465 GGATG Cac8I 814 GCCAGC GC[ACGT]^[ACGT]GC 1263 GCAAGC CfoI 369 GCGC GCG^C 818 GCGC FokI 828 CATCC GGATG(9/13) 465 GGATG HhaI 369 GCGC GCG^C 818 GCGC HinP1I 369 GCGC G^CGC 818 GCGC MaeI 563 CTAG C^TAG 1054 CTAG MaeIII 12 GTTAC ^GT[ACGT]AC 1249 GTCAC PstI 397 CTGCAG CTGCA^G 521 CTGCAG RsaI 180 GTAC GT^AC 801 GTAC StuI 303 AGGCCT AGG^CCT 491 AGGCCT TfiI 96 GAATC G^A[AT]TC 294 GATTC Tsp509I 342 AATT ^AATT 625 AATT TspRI 230 CACTG CA[GC]TG[ACGT][ACGT]^ 501 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BclI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FbaI Fnu4HI HaeIII HinfI HphI ItaI MaeII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI