(Circular) (Six-base) MAPSORT of: pFN21AE1894.seq Check: 5782 from: 1 to: 5974 pFN21AE1894 5974 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2822 2912 4037 5421 5689 1632 Size: 590 600 90 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 600 590 268 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 2134 4873 5850 1203 Size: 931 2739 977 1327 Fragments arranged by size: 2739 1327 977 931 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2792 1166 Size: 1626 4348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaI G_GGCC'C Cuts at: 2278 2278 Size: 5974 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2346 3283 3509 3661 5770 1493 Size: 853 937 226 152 2109 1697 Fragments arranged by size: 2109 1697 937 853 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2959 3299 1870 Size: 73 1016 340 4545 Fragments arranged by size: 4545 1016 340 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 3295 4750 618 Size: 325 217 627 938 570 1455 1842 Fragments arranged by size: 1842 1455 938 627 570 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2278 2937 3293 1391 Size: 649 238 659 356 4072 Fragments arranged by size: 4072 659 649 356 238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2483 2517 4165 4199 1903 Size: 34 546 34 1648 34 3678 Fragments arranged by size: 3678 1648 546 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2589 2842 3207 1548 Size: 1041 253 365 4315 Fragments arranged by size: 4315 1041 365 253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2584 5969 2584 Size: 3385 2589 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 2278 2730 3113 4035 5191 1790 Size: 488 452 383 922 1156 2573 Fragments arranged by size: 2573 1156 922 488 452 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2925 2925 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2933 2975 4634 1239 Size: 1694 42 1659 2579 Fragments arranged by size: 2579 1694 1659 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 2722 4099 4964 5262 2297 Size: 425 1377 865 298 3009 Fragments arranged by size: 3009 1377 865 425 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 4637 882 Size: 617 1380 1758 2219 Fragments arranged by size: 2219 1758 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 2554 3765 493 Size: 711 933 417 1211 2702 Fragments arranged by size: 2702 1211 933 711 417 BsaBI GATnn'nnATC Cuts at: 2682 5968 2682 Size: 3286 2688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2789 2819 1178 Size: 30 165 30 597 30 759 30 4333 Fragments arranged by size: 4333 759 597 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2789 3119 1186 Size: 925 678 330 4041 Fragments arranged by size: 4041 925 678 330 BseYI C'CCAG_C Cuts at: 2048 2995 5177 5673 2048 Size: 947 2182 496 2349 Fragments arranged by size: 2349 2182 947 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2770 1163 Size: 573 1034 4367 Fragments arranged by size: 4367 1034 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2937 4035 4120 5191 2937 Size: 1098 85 1071 3720 Fragments arranged by size: 3720 1098 1071 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2278 2730 2937 3113 3293 4035 Size: 399 250 238 452 207 176 180 742 Cuts at: 4035 4120 5191 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 250 238 207 180 176 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3243 3863 5593 3243 Size: 620 1730 3624 Fragments arranged by size: 3624 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2949 3329 844 Size: 2105 380 3489 Fragments arranged by size: 3489 2105 380 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2821 4469 4643 65 Size: 1183 1573 1648 174 1396 Fragments arranged by size: 1648 1573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 96 Size: 2088 3886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 2371 3125 794 Size: 1577 754 3643 Fragments arranged by size: 3643 1577 754 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2254 2254 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2584 3304 3671 4056 4073 5525 5611 5623 Size: 612 720 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 720 612 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2790 513 Size: 552 939 786 3697 Fragments arranged by size: 3697 939 786 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2409 487 Size: 683 56 1183 4052 Fragments arranged by size: 4052 1183 683 56 BtsI GCAGTG_nn' Cuts at: 3490 4284 4304 3490 Size: 794 20 5160 Fragments arranged by size: 5160 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 2644 3278 2644 Size: 634 5340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2834 3904 1353 Size: 1481 1070 3423 Fragments arranged by size: 3423 1481 1070 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2146 4561 4935 5081 387 Size: 1064 138 557 2415 374 146 1280 Fragments arranged by size: 2415 1280 1064 557 374 146 138 EcoICRI GAG'CTC Cuts at: 2935 2935 Size: 5974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2822 2912 2933 2975 4037 4634 Size: 393 590 600 90 21 42 1062 597 Cuts at: 4634 5421 5689 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1062 787 600 597 590 393 268 90 42 21 EcoO109I rG'GnC_Cy Cuts at: 2206 2274 2596 3195 2206 Size: 68 322 599 4985 Fragments arranged by size: 4985 599 322 68 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 2375 4480 2375 Size: 2105 3869 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 2728 2760 2765 Size: 32 135 32 1016 32 108 32 5 Cuts at: 2765 2797 4615 4647 4689 4721 1373 Size: 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 1016 135 108 42 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3932 4291 4450 518 Size: 648 1626 1140 359 159 2042 Fragments arranged by size: 2042 1626 1140 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2407 2525 2995 3390 4067 5215 5460 1606 Size: 801 118 470 395 677 1148 245 2120 Fragments arranged by size: 2120 1148 801 677 470 395 245 118 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PfoI T'CCnGG_A Cuts at: 2766 2766 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2137 2137 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 2596 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2274 2274 Size: 5974 PstI C_TGCA'G Cuts at: 830 2406 2530 3326 830 Size: 1576 124 796 3478 Fragments arranged by size: 3478 1576 796 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2407 2525 2995 2407 Size: 118 470 5386 Fragments arranged by size: 5386 470 118 SacI G_AGCT'C Cuts at: 2937 2937 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2402 2526 3219 3322 4457 5138 5329 Size: 219 1357 124 693 103 1135 681 191 Cuts at: 5329 826 Size: 1471 Fragments arranged by size: 1471 1357 1135 693 681 219 191 124 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2701 2959 4078 4979 5241 Size: 197 926 369 389 258 1119 901 262 Cuts at: 5241 820 Size: 1553 Fragments arranged by size: 1553 1119 926 901 389 369 262 258 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 2310 2498 1517 Size: 793 188 4993 Fragments arranged by size: 4993 793 188 StyI C'CwwG_G Cuts at: 513 1065 2004 2253 3413 513 Size: 552 939 249 1160 3074 Fragments arranged by size: 3074 1160 939 552 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 3242 4001 4018 4171 4356 4801 Size: 1255 559 1103 759 17 153 185 445 Cuts at: 4801 325 Size: 1498 Fragments arranged by size: 1498 1255 1103 759 559 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2805 4220 Size: 274 80 33 51 1443 207 621 1415 Cuts at: 4220 5932 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1415 621 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2817 2849 5934 5966 2817 Size: 32 3085 32 2825 Fragments arranged by size: 3085 2825 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 2959 1943 Size: 1016 4958 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 3236 4103 3236 Size: 867 5107 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI