################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AclI 373 AACGTT AA^CGTT Aor51HI 509 AGCGCT AGC^GCT AseI 865 ATTAAT AT^TAAT AsnI 865 ATTAAT AT^TAAT Asp700I 5 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 9 GTCTTC GAAGAC(2/6) BfaI 106 CTAG C^TAG BpuAI 9 GTCTTC GAAGAC(2/6) BseRI 84 CTCCTC GAGGAG(10/8) BsgI 843 CTGCAC GTGCAG(16/14) BsmAI 705 GTCTC GTCTC(1/5) BsmI 286 GAATGC GAATGC(1/-1) Bsp1407I 829 TGTACA T^GTACA BsrGI 829 TGTACA T^GTACA Cac8I 525 GCCTGC GC[ACGT]^[ACGT]GC CfoI 510 GCGC GCG^C Eco47III 509 AGCGCT AGC^GCT EcoRI 692 GAATTC G^AATTC EcoRV 145 GATATC GAT^ATC HaeII 509 AGCGCT [GA]GCGC^[CT] HaeIII 524 GGCC GG^CC HhaI 510 GCGC GCG^C HinP1I 510 GCGC G^CGC MaeI 106 CTAG C^TAG MaeII 374 ACGT A^CGT MaeIII 758 GTTAC ^GT[ACGT]AC MfeI 591 CAATTG C^AATTG MspA1I 140 CAGCTG C[AC]G^C[GT]G MunI 591 CAATTG C^AATTG MwoI 721 GCACCACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 281 CATATG CA^TATG NspI 882 ACATGC [GA]CATG^[CT] PleI 453 GAGTC GAGTC(4/5) PshBI 865 ATTAAT AT^TAAT Psp1406I 373 AACGTT AA^CGTT PstI 445 CTGCAG CTGCA^G PvuII 140 CAGCTG CAG^CTG SfaNI 643 GATGC GCATC(5/9) SfcI 445 CTGCAG C^T[GA][CT]AG SspBI 829 TGTACA T^GTACA XmnI 5 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbvI 172 GCAGC GCAGC(8/12) 512 GCTGC BsaWI 51 ACCGGA [AT]^CCGG[AT] 837 ACCGGA BsiYI 426 CCCAGTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 887 CCTTGAAATGG BslI 426 CCCAGTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 887 CCTTGAAATGG DpnII 205 GATC ^GATC 307 GATC Fnu4HI 172 GCAGC GC^[ACGT]GC 512 GCTGC HapII 52 CCGG C^CGG 838 CCGG HpaII 52 CCGG C^CGG 838 CCGG ItaI 172 GCAGC GC^[ACGT]GC 512 GCTGC MboI 205 GATC ^GATC 307 GATC MslI 281 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 675 CACTCATGTG MspI 52 CCGG C^CGG 838 CCGG NdeII 205 GATC ^GATC 307 GATC NlaIII 679 CATG CATG^ 883 CATG NlaIV 54 GGAGCC GG[ACGT]^[ACGT]CC 70 GGTTCC Sau3AI 205 GATC ^GATC 307 GATC ScaI 518 AGTACT AGT^ACT 699 AGTACT SspI 112 AATATT AAT^ATT 269 AATATT TaqI 422 TCGA T^CGA 900 TCGA TfiI 325 GAATC G^A[AT]TC 897 GATTC TseI 172 GCAGC G^C[AT]GC 512 GCTGC TthHB8I 422 TCGA T^CGA 900 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AcsI AfaI AluI ApoI BsmFI BsrI BstF5I DdeI FokI HinfI HphI MboII MnlI MseI RsaI Tru9I Tsp509I TspRI