Restriction map for
pFN21AE5351
(Circular) (Six-base) MAPSORT of: pFN21AE5351.seq Check: 9664 from: 1 to: 5623
pFN21AE5351 5623 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 278
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 2379 3750 4123 1563
Size: 816 1371 373 3063
Fragments arranged by size:
3063 1371 816 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3686 5070 5338 1632
Size: 2054 1384 268 1917
Fragments arranged by size:
2054 1917 1384 268
AfeI AGC'GCT
Cuts at: 2516 2516
Size: 5623
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 2684 1166
Size: 1518 4105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AlwNI CAG_nnn'CTG
Cuts at: 3366 4938 3366
Size: 1572 4051
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2244 2603 2697 2932 3158 3310 5419 1493
Size: 751 359 94 235 226 152 2109 1697
Fragments arranged by size:
2109 1697 751 359 235 226 152 94
AseI AT'TA_AT
Cuts at: 160 1032 2871 4177 160
Size: 872 1839 1306 1606
Fragments arranged by size:
1839 1606 1306 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2948 1870
Size: 73 1005 4545
Fragments arranged by size:
4545 1005 73
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 4399 618
Size: 325 217 627 1157 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2942 1391
Size: 1551 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2007 928
Size: 971 108 4544
Fragments arranged by size:
4544 971 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 3040 3040
Size: 5623
Bme1580I G_kGCm'C
Cuts at: 1790 3684 4840 1790
Size: 1894 1156 2573
Fragments arranged by size:
2573 1894 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2426 3354 4312
Size: 416 53 83 399 24 1009 928 958
Cuts at: 4312 442
Size: 1753
Fragments arranged by size:
1753 1009 958 928 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4283 1239
Size: 3044 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4286 882
Size: 617 2787 2219
Fragments arranged by size:
2787 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3430 3812 4447 275
Size: 53 83 186 936 1897 382 635 1451
Fragments arranged by size:
1897 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2056 2842 4056 4454 4728 4875 5286 2056
Size: 786 1214 398 274 147 411 2393
Fragments arranged by size:
2393 1214 786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5398
Fragments arranged by size:
5398 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 1186
Size: 894 4729
BseYI C'CCAG_C
Cuts at: 4826 5322 4826
Size: 496 5127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2833 1163
Size: 573 1097 3953
Fragments arranged by size:
3953 1097 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3684 3769 4840 3684
Size: 85 1071 4467
Fragments arranged by size:
4467 1071 85
BsmI GAATG_Cn'
Cuts at: 2297 3134 3227 2297
Size: 837 93 4693
Fragments arranged by size:
4693 837 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2942 3684 3769 4840 1391
Size: 399 1152 742 85 1071 2174
Fragments arranged by size:
2174 1152 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 844
Size: 2134 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 1394
Size: 429 1687 3507
Fragments arranged by size:
3507 1687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4267 4454 1700
Size: 110 2457 187 2869
Fragments arranged by size:
2869 2457 187 110
BsrGI T'GTAC_A
Cuts at: 96 2834 96
Size: 2738 2885
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 3683 4695 3683
Size: 1012 4611
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstYI r'GATC_y
Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618
Size: 981 367 385 17 1452 86 12 346
Cuts at: 5618 1972
Size: 1977
Fragments arranged by size:
1977 1452 981 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3933 3953 3139
Size: 794 20 4809
Fragments arranged by size:
4809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 3959 5588
Size: 54 1425 50 275 18 1240 889 1629
Cuts at: 5588 8
Size: 43
Fragments arranged by size:
1629 1425 1240 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 1353
Size: 2200 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3686 4283 5070 5338 1239
Size: 393 2054 597 787 268 1524
Fragments arranged by size:
2054 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2697 2932 3310 2697
Size: 235 378 5010
Fragments arranged by size:
5010 378 235
EcoRV GAT'ATC
Cuts at: 2152 2152
Size: 5623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2109 2219 2109
Size: 110 5513
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2518 4770 1678
Size: 840 2252 2531
Fragments arranged by size:
2531 2252 840
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2409 2441 2776
Size: 32 135 32 743 32 62 32 335
Cuts at: 2776 2808 2893 2925 4264 4296 4338 4370 1373
Size: 32 85 32 1339 32 42 32 2626
Fragments arranged by size:
2626 1339 743 335 135 85 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5623
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 2596 3222 2596
Size: 626 4997
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3801
Fragments arranged by size:
3801 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2290 2684 3581 3940 4099 518
Size: 648 843 281 394 897 359 159 2042
Fragments arranged by size:
2042 897 843 648 394 359 281 159
MspA1I CmG'CkG
Cuts at: 1606 2147 3039 3716 4864 5109 1606
Size: 541 892 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5071
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 3723
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2891 2981 4526 1132
Size: 97 1662 90 1545 2229
Fragments arranged by size:
2229 1662 1545 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5623
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2454 2975 830
Size: 1624 521 3478
Fragments arranged by size:
3478 1624 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
PvuII CAG'CTG
Cuts at: 2147 2147
Size: 5623
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 2525 2706 3871 5583 2525
Size: 181 1165 1712 2565
Fragments arranged by size:
2565 1712 1165 181
SfcI C'TryA_G
Cuts at: 826 1045 2450 2971 4106 4787 4978 826
Size: 219 1405 521 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 681 521 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5623
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3727 4628 4890 820
Size: 197 926 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SspI AAT'ATT
Cuts at: 5 52 2119 2276 3547 5
Size: 47 2067 157 1271 2081
Fragments arranged by size:
2081 2067 1271 157 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5623
StyI C'CwwG_G
Cuts at: 513 1065 3062 513
Size: 552 1997 3074
Fragments arranged by size:
3074 1997 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2646 3650 3667 3820 4005 4450 325
Size: 1255 1066 1004 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1066 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 2657 2704
Size: 274 80 33 51 1443 546 134 47
Cuts at: 2704 2834 3869 5581 96
Size: 130 1035 1712 138
Fragments arranged by size:
1712 1443 1035 546 274 138 134 130
80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5583 5615 5583
Size: 32 5591
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 2014 3752 2014
Size: 1738 3885
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 276
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI