Restriction map for
pFN21AE2358
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI Tru9I TspRI Tth111I TthHB8I
XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 689 GAATTT [GA]^AATT[CT]
AcyI 826 GGCGTC G[GA]^CG[CT]C
AfaI 618 GTAC GT^AC
AflIII 485 ACATGT A^C[GA][CT]GT
AgeI 51 ACCGGT A^CCGGT
AlwI 15 GATCC GGATC(4/5)
AlwNI 455 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 655 CCTGAGG CC^T[ACGT]AGG
Aor51HI 377 AGCGCT AGC^GCT
ApaI 463 GGGCCC GGGCC^C
ApoI 689 GAATTT [GA]^AATT[CT]
Asp700I 915 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 580 GAAGAC GAAGAC(2/6)
BglI 228 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 580 GAAGAC GAAGAC(2/6)
BsaHI 826 GGCGTC G[GA]^CG[CT]C
BsaI 808 GAGACC GGTCTC(1/5)
BsaWI 51 ACCGGT [AT]^CCGG[AT]
BseRI 1038 GAGGAG GAGGAG(10/8)
BsmBI 601 GAGACG CGTCTC(1/5)
BspLU11I 485 ACATGT A^CATGT
BstEII 849 GGTGACC G^GT[ACGT]ACC
BstPI 849 GGTGACC G^GT[ACGT]ACC
BstXI 590 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 655 CCTGAGG CC^T[ACGT]AGG
Eco47III 377 AGCGCT AGC^GCT
Eco81I 655 CCTGAGG CC^T[ACGT]AGG
EcoNI 895 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 849 GGTGACC G^GT[ACGT]ACC
HaeII 377 AGCGCT [GA]GCGC^[CT]
Hin1I 826 GGCGTC G[GA]^CG[CT]C
HincII 829 GTCAAC GT[CT]^[GA]AC
HindII 829 GTCAAC GT[CT]^[GA]AC
HinfI 59 GAATC G^A[ACGT]TC
KspI 26 CCGCGG CCGC^GG
MaeII 290 ACGT A^CGT
PinAI 51 ACCGGT A^CCGGT
PpuMI 903 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 618 GTAC GT^AC
SacI 706 GAGCTC GAGCT^C
SacII 26 CCGCGG CCGC^GG
ScaI 617 AGTACT AGT^ACT
SmlI 223 CTTGAG C^T[CT][GA]AG
SphI 1122 GCATGC GCATG^C
StuI 1010 AGGCCT AGG^CCT
TfiI 59 GAATC G^A[AT]TC
Tsp509I 690 AATT ^AATT
XcmI 1175 CCATGGCTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 915 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 706 GAGCTC G[AT]GC[AT]^C
945 GAGCAC
BfaI 76 CTAG C^TAG
524 CTAG
BsiHKAI 706 GAGCTC G[AT]GC[AT]^C
945 GAGCAC
BsrI 496 ACTGG ACTGG(1/-1)
983 ACTGG
BssSI 373 CACGAG CACGAG(-5/-1)
1237 CACGAG
DpnII 15 GATC ^GATC
1053 GATC
DraII 463 GGGCCCT [GA]G^G[ACGT]CC[CT]
903 AGGACCT
EcoO109I 463 GGGCCCT [GA]G^G[ACGT]CC[CT]
903 AGGACCT
MaeI 76 CTAG C^TAG
524 CTAG
MboI 15 GATC ^GATC
1053 GATC
MspA1I 26 CCGCGG C[AC]G^C[GT]G
33 CCGCTG
NdeII 15 GATC ^GATC
1053 GATC
NspI 485 ACATGT [GA]CATG^[CT]
1122 GCATGC
PflMI 454 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
670 CCAGGAGTTGG
SapI 915 GAAGAGC GCTCTTC(1/4)
441 GCTCTTC
Sau3AI 15 GATC ^GATC
1053 GATC
SmaI 346 CCCGGG CCC^GGG
394 CCCGGG
Tsp45I 94 GTCAC ^GT[GC]AC
850 GTGAC
Van91I 454 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
670 CCAGGAGTTGG
XmaCI 346 CCCGGG C^CCGGG
394 CCCGGG
XmaI 346 CCCGGG C^CCGGG
394 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
Sau96I ScrFI SfaNI StyI TseI