(Circular) (Six-base) MAPSORT of: pFN21AE2358.seq Check: 9040 from: 1 to: 5968 pFN21AE2358 5968 bp With 182 enzymes: SgfI * July 7, 2011 17:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 4031 5415 5683 1632 Size: 1127 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 1127 268 AfeI AGC'GCT Cuts at: 2384 2384 Size: 5968 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 2490 4867 5844 1203 Size: 1287 2377 977 1327 Fragments arranged by size: 2377 1327 1287 977 AgeI A'CCGG_T Cuts at: 2056 4799 2056 Size: 2743 3225 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 3234 1166 Size: 2068 3900 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 2465 3711 5283 2465 Size: 1246 1572 3150 Fragments arranged by size: 3150 1572 1246 ApaI G_GGCC'C Cuts at: 2472 2472 Size: 5968 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2694 3277 3503 3655 5764 1493 Size: 1201 583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1201 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2261 2351 2399 3293 1870 Size: 73 318 90 48 894 4545 Fragments arranged by size: 4545 894 318 90 73 48 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3289 4744 618 Size: 325 217 627 1502 1455 1842 Fragments arranged by size: 1842 1502 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2235 2262 2472 2715 3168 3175 3287 Size: 640 204 27 210 243 453 7 112 Cuts at: 3287 1391 Size: 4072 Fragments arranged by size: 4072 640 453 243 210 204 112 27 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2592 928 Size: 971 693 4304 Fragments arranged by size: 4304 971 693 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 5070 3860 Size: 1210 4758 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2239 4579 Size: 107 122 71 631 605 337 230 2340 Cuts at: 4579 136 Size: 1525 Fragments arranged by size: 2340 1525 631 605 337 230 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 2472 3084 4029 5185 1790 Size: 682 612 945 1156 2573 Fragments arranged by size: 2573 1156 945 682 612 BmgBI CAC'GTC Cuts at: 1318 2296 1318 Size: 978 4990 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3699 4657 442 Size: 416 53 83 399 24 2282 958 1753 Fragments arranged by size: 2282 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2462 2462 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2210 2342 2523 2537 2639 3194 4628 Size: 806 165 132 181 14 102 555 1434 Cuts at: 4628 1239 Size: 2579 Fragments arranged by size: 2579 1434 806 555 181 165 132 102 14 Bpu10I CC'TnA_GC Cuts at: 3186 3186 Size: 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 4093 4958 5256 2249 Size: 1844 865 298 2961 Fragments arranged by size: 2961 1844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 4631 882 Size: 617 1308 1824 2219 Fragments arranged by size: 2219 1824 1308 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2832 3775 4157 4792 Size: 53 83 186 936 1299 943 382 635 Cuts at: 4792 275 Size: 1451 Fragments arranged by size: 1451 1299 943 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2056 4401 4799 5073 5220 5631 2056 Size: 2345 398 274 147 411 2393 Fragments arranged by size: 2393 2345 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3058 1186 Size: 1872 4096 BseYI C'CCAG_C Cuts at: 2046 2206 2540 5171 5667 2046 Size: 160 334 2631 496 2347 Fragments arranged by size: 2631 2347 496 334 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5395 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2715 2954 4029 4114 5185 2715 Size: 239 1075 85 1071 3498 Fragments arranged by size: 3498 1075 1071 239 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 BsmBI CGTCTCn'nnnn_ Cuts at: 2600 2600 Size: 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2235 2262 2472 2715 2954 3084 Size: 399 241 204 27 210 243 239 130 Cuts at: 3084 3168 3175 3287 4029 4114 5185 1391 Size: 84 7 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 243 241 239 210 204 130 112 85 84 27 7 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3323 844 Size: 2479 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4463 4637 65 Size: 1183 3215 174 1396 Fragments arranged by size: 3215 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2095 2416 4612 4799 1700 Size: 110 246 39 321 2196 187 2869 Fragments arranged by size: 2869 2196 321 246 187 110 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 2107 2133 2153 2194 2196 2422 1925 Size: 182 26 20 41 2 226 5471 Fragments arranged by size: 5471 226 182 41 26 20 2 BssSI C'ACGA_G Cuts at: 2378 3242 4028 5040 2378 Size: 864 786 1012 3306 Fragments arranged by size: 3306 1012 864 786 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 2854 1184 Size: 1670 4298 BstXI CCAn_nnnn'nTGG Cuts at: 2602 2602 Size: 5968 BstYI r'GATC_y Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963 Size: 1326 367 385 17 1452 86 12 346 Cuts at: 5963 1972 Size: 1977 Fragments arranged by size: 1977 1452 1326 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2661 2661 Size: 5968 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2235 2313 2601 2724 3037 Size: 552 939 27 204 78 288 123 313 Cuts at: 3037 3180 513 Size: 143 3301 Fragments arranged by size: 3301 939 552 313 288 204 143 123 78 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 3484 4278 4298 3484 Size: 794 20 5154 Fragments arranged by size: 5154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2310 2335 2778 Size: 54 1425 50 275 18 480 25 443 Cuts at: 2778 3238 3415 4304 5933 8 Size: 460 177 889 1629 43 Fragments arranged by size: 1629 1425 889 480 460 443 275 177 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 2915 2987 3898 1353 Size: 1100 462 72 911 3423 Fragments arranged by size: 3423 1100 911 462 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2713 2713 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2210 2342 2523 2537 2639 2759 Size: 393 413 165 132 181 14 102 120 Cuts at: 2759 3194 4031 4628 5415 5683 1239 Size: 435 837 597 787 268 1524 Fragments arranged by size: 1524 837 787 597 435 413 393 268 181 165 132 120 102 14 EcoNI CCTnn'n_nnAGG Cuts at: 2904 2904 Size: 5968 EcoO109I rG'GnC_Cy Cuts at: 2469 2909 2469 Size: 440 5528 EcoRI G'AATT_C Cuts at: 3277 3655 3277 Size: 378 5590 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 2386 5115 1678 Size: 708 2729 2531 Fragments arranged by size: 2729 2531 708 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 4609 4641 4683 Size: 32 135 32 1027 32 1978 32 42 Cuts at: 4683 4715 1373 Size: 32 2626 Fragments arranged by size: 2626 1978 1027 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2836 3312 3558 669 Size: 988 281 898 476 246 3079 Fragments arranged by size: 3079 988 898 476 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5082 5266 1305 Size: 3777 184 2007 Fragments arranged by size: 3777 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 2780 3240 10 Size: 54 1425 343 505 443 460 2738 Fragments arranged by size: 2738 1425 505 460 443 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2852 2933 3234 3926 4285 4444 518 Size: 648 1686 81 301 692 359 159 2042 Fragments arranged by size: 2042 1686 692 648 359 301 159 81 MspA1I CmG'CkG Cuts at: 1606 2033 2040 3384 4061 5209 5454 1606 Size: 427 7 1344 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 677 427 245 7 NcoI C'CATG_G Cuts at: 513 1065 2004 2235 2601 2724 3037 3180 513 Size: 552 939 231 366 123 313 143 3301 Fragments arranged by size: 3301 939 552 366 313 231 143 123 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2494 3131 3326 4871 1132 Size: 97 1265 637 195 1545 2229 Fragments arranged by size: 2229 1545 1265 637 195 97 PasI CC'CwG_GG Cuts at: 1348 3168 1348 Size: 1820 4148 PciI A'CATG_T Cuts at: 2490 4867 2490 Size: 2377 3591 PflMI CCAn_nnn'nTGG Cuts at: 1108 2465 2681 1108 Size: 1357 216 4395 Fragments arranged by size: 4395 1357 216 PfoI T'CCnGG_A Cuts at: 2022 2674 2022 Size: 652 5316 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2909 2909 Size: 5968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 2468 2468 Size: 5968 PstI C_TGCA'G Cuts at: 830 3320 830 Size: 2490 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 SacI G_AGCT'C Cuts at: 2715 2715 Size: 5968 SacII CC_GC'GG Cuts at: 2034 2034 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2453 2915 2453 Size: 462 5506 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 2624 4216 5928 2624 Size: 1592 1712 2664 Fragments arranged by size: 2664 1712 1592 SfcI C'TryA_G Cuts at: 826 1045 3316 4451 5132 5323 826 Size: 219 2271 1135 681 191 1471 Fragments arranged by size: 2271 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 2353 2401 3295 1872 Size: 481 48 894 4545 Fragments arranged by size: 4545 894 481 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 4072 4973 5235 820 Size: 197 926 285 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 285 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3131 3326 1132 Size: 1999 195 3774 Fragments arranged by size: 3774 1999 195 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 3017 1517 Size: 1500 4468 StyI C'CwwG_G Cuts at: 513 1065 2004 2173 2235 2253 2271 2307 2601 Size: 552 939 169 62 18 18 36 294 Cuts at: 2601 2724 3037 3132 3180 3407 513 Size: 123 313 95 48 227 3074 Fragments arranged by size: 3074 939 552 313 294 227 169 123 95 62 48 36 18 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3224 3995 4012 4165 4350 4795 325 Size: 1255 1644 771 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 4214 5926 Size: 274 80 33 51 1443 645 1592 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1712 1592 1443 645 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5928 5960 5928 Size: 32 5936 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3187 3187 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 2351 2399 3293 1870 Size: 481 48 894 4545 Fragments arranged by size: 4545 894 481 48 XmnI GAAnn'nnTTC Cuts at: 2924 4097 2924 Size: 1173 4795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI