################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanII BbeI BbrPI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CfoI ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoT22I FauI FseI FspI HaeII HhaI HinP1I HindIII KasI KpnI KspI MaeII MamI MluI MroI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 991 GGCGTC G[GA]^CG[CT]C AgeI 2006 ACCGGT A^CCGGT Alw44I 565 GTGCAC G^TGCAC AocI 1078 CCTAAGG CC^T[ACGT]AGG ApaLI 565 GTGCAC G^TGCAC Asp700I 2016 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 717 TGGCCA TGG^CCA BbvCI 1711 CCTCAGC CCTCAGC(-5/-2) BclI 1341 TGATCA T^GATCA BglI 1974 GCCCTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1582 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1582 GCTGAGC GC^T[ACGT]AGC BsaHI 991 GGCGTC G[GA]^CG[CT]C BsaWI 2006 ACCGGT [AT]^CCGG[AT] BspHI 2046 TCATGA T^CATGA BsrFI 2006 ACCGGT [GA]^CCGG[CT] BstEII 1238 GGTTACC G^GT[ACGT]ACC BstPI 1238 GGTTACC G^GT[ACGT]ACC BstXI 1984 CCAGGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1078 CCTAAGG CC^T[ACGT]AGG CelII 1582 GCTGAGC GC^T[ACGT]AGC Cfr10I 2006 ACCGGT [GA]^CCGG[CT] DraIII 568 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 717 TGGCCA [CT]^GGCC[GA] Eco81I 1078 CCTAAGG CC^T[ACGT]AGG EcoO65I 1238 GGTTACC G^GT[ACGT]ACC EcoRV 316 GATATC GAT^ATC FbaI 1341 TGATCA T^GATCA HgaI 992 GCGTC GACGC(5/10) Hin1I 991 GGCGTC G[GA]^CG[CT]C HincII 508 GTTAAC GT[CT]^[GA]AC HindII 508 GTTAAC GT[CT]^[GA]AC HpaI 508 GTTAAC GTT^AAC MfeI 701 CAATTG C^AATTG MluNI 717 TGGCCA TGG^CCA MscI 717 TGGCCA TGG^CCA MslI 1011 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 701 CAATTG C^AATTG PflMI 1740 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2006 ACCGGT A^CCGGT PstI 737 CTGCAG CTGCA^G RcaI 2046 TCATGA T^CATGA ScaI 1829 AGTACT AGT^ACT Van91I 1740 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2016 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 595 GTCTAC GT^[AC][GT]AC 1387 GTAGAC AciI 1597 CCGC CCGC(-3/-1) 876 GCGG AlwNI 740 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 1286 CAGATGCTG AvaII 307 GGACC G^G[AT]CC 1086 GGACC BamHI 188 GGATCC G^GATCC 1887 GGATCC BanI 1002 GGCACC G^G[CT][GA]CC 1946 GGCACC BbsI 1423 GAAGAC GAAGAC(2/6) 1512 GTCTTC BcnI 45 CCCGG CC^[GC]GG 1171 CCGGG BpmI 25 CTCCAG CTGGAG(16/14) 1675 CTGGAG BpuAI 1423 GAAGAC GAAGAC(2/6) 1512 GTCTTC BsaI 1678 GAGACC GGTCTC(1/5) 2009 GGTCTC BsmAI 1678 GAGAC GTCTC(1/5) 2010 GTCTC BsmI 267 GAATGC GAATGC(1/-1) 361 GCATTC BsrDI 1124 CATTGC GCAATG(2/0) 1970 CATTGC DraI 1410 TTTAAA TTT^AAA 1687 TTTAAA NciI 45 CCCGG CC^[GC]GG 1171 CCGGG NspI 109 ACATGC [GA]CATG^[CT] 541 GCATGT SexAI 1458 ACCTGGT A^CC[AT]GGT 1681 ACCAGGT StuI 607 AGGCCT AGG^CCT 1091 AGGCCT Tsp45I 578 GTGAC ^GT[GC]AC 1550 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AfaI AluI AlwI AspHI BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NheI NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII