Restriction map for
pFN21AE4204
(Circular) (Six-base) MAPSORT of: pFN21AE4204.seq Check: 1615 from: 1 to: 6838
pFN21AE4204 6838 bp
With 182 enzymes: SgfI *
March 28, 2012 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2511 2710 3652 2511
Size: 199 942 5697
Fragments arranged by size:
5697 942 199
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 3393 4181 1937
Size: 664 792 788 4594
Fragments arranged by size:
4594 792 788 664
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2897 3504 3748 4140 4146 4901 6285
Size: 504 761 607 244 392 6 755 1384
Cuts at: 6285 6553 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 761 755 607 504 392 268
244 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 4011 5669 4011
Size: 1658 5180
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 1184
Size: 1782 5056
AlwNI CAG_nnn'CTG
Cuts at: 2750 3296 4581 6153 2750
Size: 546 1285 1572 3435
Fragments arranged by size:
3435 1572 1285 546
ApaLI G'TGCA_C
Cuts at: 2570 4895 6051 2570
Size: 2325 1156 3357
Fragments arranged by size:
3357 2325 1156
ApoI r'AATT_y
Cuts at: 1493 4147 4373 4525 6634 1493
Size: 2654 226 152 2109 1697
Fragments arranged by size:
2654 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 4163 1870
Size: 73 2220 4545
Fragments arranged by size:
4545 2220 73
BamHI G'GATC_C
Cuts at: 2193 3892 4168 4535 2193
Size: 1699 276 367 4496
Fragments arranged by size:
4496 1699 367 276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 3951 4159 5614 618
Size: 325 217 627 1220 944 208 1455 1842
Fragments arranged by size:
1842 1455 1220 944 627 325 217 208
BanII G_rGCy'C
Cuts at: 1391 4157 1391
Size: 2766 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3435 3510 928
Size: 971 1536 75 4256
Fragments arranged by size:
4256 1536 971 75
BbvCI CC'TCA_GC
Cuts at: 3717 3717
Size: 6838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5029 5063 1903
Size: 34 50 34 3008 34 3678
Fragments arranged by size:
3678 3008 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 3346 1548
Size: 1798 5040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 5449 136
Size: 107 122 71 631 605 2313 1464 1525
Fragments arranged by size:
2313 1525 1464 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6833 6833
Size: 6838
BlpI GC'TnA_GC
Cuts at: 3588 4255 3588
Size: 667 6171
Bme1580I G_kGCm'C
Cuts at: 1790 2574 4899 6055 1790
Size: 784 2325 1156 2573
Fragments arranged by size:
2573 2325 1156 784
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6838
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3428 3484 4072
Size: 416 53 83 399 24 2011 56 588
Cuts at: 4072 4569 5527 442
Size: 497 958 1753
Fragments arranged by size:
2011 1753 958 588 497 416 399 83
56 53 24
BmtI G_CTAG'C
Cuts at: 1056 2127 2672 2676 1056
Size: 1071 545 4 5218
Fragments arranged by size:
5218 1071 545 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 3701 5498 1239
Size: 776 1686 1797 2579
Fragments arranged by size:
2579 1797 1686 776
Bpu10I CC'TnA_GC
Cuts at: 2535 3717 2535
Size: 1182 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 2896 3659 4963 5828 6126 2825
Size: 71 763 1304 865 298 3537
Fragments arranged by size:
3537 1304 865 763 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3677 4020 5501 882
Size: 617 2178 343 1481 2219
Fragments arranged by size:
2219 2178 1481 617 343
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2997 4645 5027 5662
Size: 53 83 186 936 1464 1648 382 635
Cuts at: 5662 275
Size: 1451
Fragments arranged by size:
1648 1464 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4011 5271 5669 5943 6090 6501 4011
Size: 1260 398 274 147 411 4348
Fragments arranged by size:
4348 1260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2855 2885 3674 3704 1178
Size: 30 165 30 1452 30 789 30 4312
Fragments arranged by size:
4312 1452 789 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6838
BseYI C'CCAG_C
Cuts at: 2606 3548 3864 4042 6041 6537 2606
Size: 942 316 178 1999 496 2907
Fragments arranged by size:
2907 1999 942 496 316 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2236 2674 3183 1163
Size: 573 500 438 509 4818
Fragments arranged by size:
4818 573 509 500 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2562 2574 2665 4899 4984 6055 2046
Size: 516 12 91 2234 85 1071 2829
Fragments arranged by size:
2829 2234 1071 516 91 85 12
BsmI GAATG_Cn'
Cuts at: 2278 2366 4349 4442 2278
Size: 88 1983 93 4674
Fragments arranged by size:
4674 1983 93 88
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2562 2574 2665 4157 4899 4984
Size: 399 256 516 12 91 1492 742 85
Cuts at: 4984 6055 1391
Size: 1071 2174
Fragments arranged by size:
2174 1492 1071 742 516 399 256 91
85 12
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4051 4727 6457 4051
Size: 676 1730 4432
Fragments arranged by size:
4432 1730 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2710 3652 3815 4193 844
Size: 1667 199 942 163 378 3489
Fragments arranged by size:
3489 1667 942 378 199 163
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3128 3974 5333 5507 65
Size: 1183 1880 846 1359 174 1396
Fragments arranged by size:
1880 1396 1359 1183 846 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4011 5482 5669 1700
Size: 110 2201 1471 187 2869
Fragments arranged by size:
2869 2201 1471 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2282 2372 3906 794
Size: 1488 90 1534 3726
Fragments arranged by size:
3726 1534 1488 90
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 3243 1184
Size: 2059 4779
BstXI CCAn_nnnn'nTGG
Cuts at: 3996 3996
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 2193 2402 2470 3874 3892 4168 4535 4920
Size: 221 209 68 1404 18 276 367 385
Cuts at: 4920 4937 6389 6475 6487 6833 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1404 385 367 346 276 221
209 86 68 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 3084 3084
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 4354 5148 5168 2751
Size: 1603 794 20 4421
Fragments arranged by size:
4421 1603 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 3417 3694 4142 3417
Size: 277 448 6113
Fragments arranged by size:
6113 448 277
DraIII CAC_nnn'GTG
Cuts at: 2578 2578
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2722 4285 5174
Size: 54 1425 50 275 18 892 1563 889
Cuts at: 5174 6803 8
Size: 1629 43
Fragments arranged by size:
1629 1563 1425 892 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 3616 4122 4768 1353
Size: 872 1391 506 646 3423
Fragments arranged by size:
3423 1391 872 646 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2136 2897 3504 3701 3748 4140
Size: 393 383 121 761 607 197 47 392
Cuts at: 4140 4146 4901 5498 6285 6553 1239
Size: 6 755 597 787 268 1524
Fragments arranged by size:
1524 787 761 755 607 597 393 392
383 268 197 121 47 6
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
EcoRV GAT'ATC
Cuts at: 2323 2323
Size: 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2333 2333
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 3308 3340 3548
Size: 32 135 32 613 32 1091 32 208
Cuts at: 3548 3580 5479 5511 5553 5585 1373
Size: 32 1899 32 42 32 2626
Fragments arranged by size:
2626 1899 1091 613 208 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2515 4182 4428 669
Size: 988 281 577 1667 246 3079
Fragments arranged by size:
3079 1667 988 577 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 2515 4428 2515
Size: 1913 4925
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 2706 4437 2706
Size: 1731 5107
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 3632 5952 6136 1305
Size: 1536 791 2320 184 2007
Fragments arranged by size:
2320 2007 1536 791 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2724 10
Size: 54 1425 343 892 4124
Fragments arranged by size:
4124 1425 892 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 4796 5155 5314 518
Size: 648 1854 1776 359 159 2042
Fragments arranged by size:
2042 1854 1776 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4254 4931 6079 6324 1606
Size: 2648 677 1148 245 2120
Fragments arranged by size:
2648 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6286
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 2123 2668 2672 1052
Size: 1071 545 4 5218
Fragments arranged by size:
5218 1071 545 4
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2118 2550 4196 5741 1132
Size: 97 889 432 1646 1545 2229
Fragments arranged by size:
2229 1646 1545 889 432 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6838
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3751 1108
Size: 2643 4195
PfoI T'CCnGG_A
Cuts at: 3678 3678
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PstI C_TGCA'G
Cuts at: 830 2746 4190 830
Size: 1916 1444 3478
Fragments arranged by size:
3478 1916 1444
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 3836 5086 6798 3836
Size: 1250 1712 3876
Fragments arranged by size:
3876 1712 1250
SexAI A'CCwGG_T
Cuts at: 3463 3686 3463
Size: 223 6615
SfcI C'TryA_G
Cuts at: 826 1045 2238 2742 3883 4186 5321 6002 6193
Size: 219 1193 504 1141 303 1135 681 191
Cuts at: 6193 826
Size: 1471
Fragments arranged by size:
1471 1193 1141 1135 681 504 303 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 2911 3638 4942 5843 6105
Size: 197 926 861 107 727 1304 901 262
Cuts at: 6105 820
Size: 1553
Fragments arranged by size:
1553 1304 926 901 861 727 262 197
107
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 2614 3098 1517
Size: 1097 484 5257
Fragments arranged by size:
5257 1097 484
StyI C'CwwG_G
Cuts at: 513 1065 2352 2484 3387 4277 513
Size: 552 1287 132 903 890 3074
Fragments arranged by size:
3074 1287 903 890 552 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4865 4882 5035 5220 5665 325
Size: 1255 3285 17 153 185 445 1498
Fragments arranged by size:
3285 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 3723 3834
Size: 274 80 33 51 1443 1646 100 111
Cuts at: 3834 5084 6796 96
Size: 1250 1712 138
Fragments arranged by size:
1712 1646 1443 1250 274 138 111 100
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2855 2887 6798 6830 2855
Size: 32 3911 32 2863
Fragments arranged by size:
3911 2863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2322 2491 2897 3787 2322
Size: 169 406 890 5373
Fragments arranged by size:
5373 890 406 169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4967 4025
Size: 942 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI