################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 601 GTGCAC G^TGCAC Aor51HI 1157 AGCGCT AGC^GCT ApaI 2057 GGGCCC GGGCC^C ApaLI 601 GTGCAC G^TGCAC BamHI 203 GGATCC G^GATCC BfaI 1423 CTAG C^TAG BsaBI 39 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1651 GAGACG CGTCTC(1/5) BsmI 476 GCATTC GAATGC(1/-1) BspHI 2304 TCATGA T^CATGA BsrDI 398 GCAATG GCAATG(2/0) BsrFI 98 ACCGGC [GA]^CCGG[CT] BsrI 2438 CCAGT ACTGG(1/-1) BstAPI 171 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 98 ACCGGC [GA]^CCGG[CT] DraI 2314 TTTAAA TTT^AAA Eco47III 1157 AGCGCT AGC^GCT EcoRI 586 GAATTC G^AATTC HincII 715 GTCAAC GT[CT]^[GA]AC HindII 715 GTCAAC GT[CT]^[GA]AC KspI 48 CCGCGG CCGC^GG MaeI 1423 CTAG C^TAG MaeIII 2441 GTGAC ^GT[ACGT]AC MamI 39 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 677 CATATG CA^TATG NruI 1034 TCGCGA TCG^CGA PleI 2364 GAGTC GAGTC(4/5) PpuMI 833 GGGTCCT [GA]G^G[AT]CC[CT] PstI 2006 CTGCAG CTGCA^G RcaI 2304 TCATGA T^CATGA SacII 48 CCGCGG CCGC^GG SmaI 830 CCCGGG CCC^GGG SmlI 656 CTCAAG C^T[CT][GA]AG StuI 2387 AGGCCT AGG^CCT TaqI 1194 TCGA T^CGA Tsp45I 2441 GTGAC ^GT[GC]AC TthHB8I 1194 TCGA T^CGA XmaCI 830 CCCGGG C^CCGGG XmaI 830 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 968 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1642 CAGTTCCTG BciVI 469 GGATAC GTATCC(6/5) 1709 GGATAC BglII 1983 AGATCT A^GATCT 2493 AGATCT Bsp1407I 1760 TGTACA T^GTACA 2372 TGTACA BspMI 104 ACCTGC ACCTGC(4/8) 525 GCAGGT BsrGI 1760 TGTACA T^GTACA 2372 TGTACA BstXI 1465 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2383 CCAAAGGCCTGG FauI 146 CCCGC CCCGC(4/6) 578 CCCGC NcoI 440 CCATGG C^CATGG 2075 CCATGG NspI 1746 GCATGT [GA]CATG^[CT] 2291 ACATGC PflMI 186 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1088 CCAAGGCATGG SacI 1099 GAGCTC GAGCT^C 1504 GAGCTC SexAI 188 ACCTGGT A^CC[AT]GGT 1172 ACCTGGT SfcI 1944 CTACAG C^T[GA][CT]AG 2006 CTGCAG SspBI 1760 TGTACA T^GTACA 2372 TGTACA Van91I 186 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1088 CCAAGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI XcmI XhoII XmnI