Restriction map for
pFN21AE5817
(Circular) (Six-base) MAPSORT of: pFN21AE5817.seq Check: 6173 from: 1 to: 7246
pFN21AE5817 7246 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2118 2118
Size: 7246
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5056 278
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4589 1937
Size: 2652 4594
Acc65I G'GTAC_C
Cuts at: 4567 4567
Size: 7246
AclI AA'CG_TT
Cuts at: 1563 5373 5746 1563
Size: 3810 373 3063
Fragments arranged by size:
3810 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 3403 3656 3864 4241 4479 5309 6693
Size: 476 1295 253 208 377 238 830 1384
Cuts at: 6693 6961 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1295 830 476 377 268 253
238 208
AfeI AGC'GCT
Cuts at: 3164 3164
Size: 7246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7049
AflIII A'CryG_T
Cuts at: 1203 6145 7122 1203
Size: 4942 977 1327
Fragments arranged by size:
4942 1327 977
AgeI A'CCGG_T
Cuts at: 6077 6077
Size: 7246
AhdI GACnn_n'nnGTC
Cuts at: 5975 5975
Size: 7246
AleI CACnn'nnGTG
Cuts at: 1166 2663 2693 3881 1166
Size: 1497 30 1188 4531
Fragments arranged by size:
4531 1497 1188 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7246
AlwNI CAG_nnn'CTG
Cuts at: 2978 3652 4989 6561 2978
Size: 674 1337 1572 3663
Fragments arranged by size:
3663 1572 1337 674
ApaI G_GGCC'C
Cuts at: 4066 4066
Size: 7246
ApaLI G'TGCA_C
Cuts at: 2606 5303 6459 2606
Size: 2697 1156 3393
Fragments arranged by size:
3393 2697 1156
ApoI r'AATT_y
Cuts at: 1493 2270 2591 4436 4555 4781 4933 7042 1493
Size: 777 321 1845 119 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 777 321 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 5800 160
Size: 872 4768 1606
Fragments arranged by size:
4768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 2835 4027 4429 4571 1870
Size: 73 802 90 1192 402 142 4545
Fragments arranged by size:
4545 1192 802 402 142 90 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3707 3740 3707
Size: 33 7213
BamHI G'GATC_C
Cuts at: 2208 4576 4943 2208
Size: 2368 367 4511
Fragments arranged by size:
4511 2368 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2749 4016 4567 6022
Size: 325 217 627 319 643 1267 551 1455
Cuts at: 6022 618
Size: 1842
Fragments arranged by size:
1842 1455 1267 643 627 551 325 319
217
BanII G_rGCy'C
Cuts at: 1391 3108 3513 4066 4565 1391
Size: 1717 405 553 499 4072
Fragments arranged by size:
4072 1717 553 499 405
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3122 3404 3540 3586 928
Size: 971 1223 282 136 46 4588
Fragments arranged by size:
4588 1223 971 282 136 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5437 5471 1903
Size: 34 3500 34 3678
Fragments arranged by size:
3678 3500 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2468 3708 5138 6348 2468
Size: 1240 1430 1210 3366
Fragments arranged by size:
3366 1430 1240 1210
BclI T'GATC_A
Cuts at: 1548 2304 3756 3861 3873 4234 1548
Size: 756 1452 105 12 361 4560
Fragments arranged by size:
4560 1452 756 361 105 12
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5857 136
Size: 107 122 71 631 605 4185 1525
Fragments arranged by size:
4185 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3988 4498 7241 3988
Size: 510 2743 3993
Fragments arranged by size:
3993 2743 510
BlpI GC'TnA_GC
Cuts at: 4663 4663
Size: 7246
Bme1580I G_kGCm'C
Cuts at: 1790 2243 2610 2700 2752 4066 5307 6463 1790
Size: 453 367 90 52 1314 1241 1156 2573
Fragments arranged by size:
2573 1314 1241 1156 453 367 90 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4977 5935 442
Size: 416 53 83 399 24 3560 958 1753
Fragments arranged by size:
3560 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7246
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 7246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3179 3674 4426 5906 1239
Size: 1940 495 752 1480 2579
Fragments arranged by size:
2579 1940 1480 752 495
Bpu10I CC'TnA_GC
Cuts at: 3048 3551 3048
Size: 503 6743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2646 5371 6236 6534 2646
Size: 2725 865 298 3358
Fragments arranged by size:
3358 2725 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5909 882
Size: 617 4410 2219
Fragments arranged by size:
4410 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5037 493
Size: 711 3833 2702
Fragments arranged by size:
3833 2702 711
BsaBI GATnn'nnATC
Cuts at: 2048 7240 2048
Size: 5192 2054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5053 5435 6070 275
Size: 53 83 186 936 3520 382 635 1451
Fragments arranged by size:
3520 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5679 6077 6351 6498 6909 5679
Size: 398 274 147 411 6016
Fragments arranged by size:
6016 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3647 3671 3677 3701 1178
Size: 30 165 30 2244 24 6 24 4723
Fragments arranged by size:
4723 2244 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7246
BseYI C'CCAG_C
Cuts at: 4115 6449 6945 4115
Size: 2334 496 4416
Fragments arranged by size:
4416 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2991 3018 1163
Size: 573 360 895 27 5391
Fragments arranged by size:
5391 895 573 360 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 5457 6485 1540
Size: 275 186 2695 761 1028 2301
Fragments arranged by size:
2695 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 3108 3147 3513 5307 5392 6463 2610
Size: 498 39 366 1794 85 1071 3393
Fragments arranged by size:
3393 1794 1071 498 366 85 39
BsmI GAATG_Cn'
Cuts at: 2481 4757 4850 2481
Size: 2276 93 4877
Fragments arranged by size:
4877 2276 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3650 3650
Size: 7246
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2610 2700 2752 3108 3147 3513
Size: 399 453 367 90 52 356 39 366
Cuts at: 3513 4066 4565 5307 5392 6463 1391
Size: 553 499 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 499 453 399 367
366 356 90 85 52 39
BspEI T'CCGG_A
Cuts at: 6909 6909
Size: 7246
BspHI T'CATG_A
Cuts at: 4309 5135 6865 4309
Size: 826 1730 4690
Fragments arranged by size:
4690 1730 826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2521 4601 844
Size: 1274 403 2080 3489
Fragments arranged by size:
3489 2080 1274 403
BsrBI CCG'CTC
Cuts at: 1394 1823 5133 1394
Size: 429 3310 3507
Fragments arranged by size:
3507 3310 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2410 5741 5915 65
Size: 1183 1162 3331 174 1396
Fragments arranged by size:
3331 1396 1183 1162 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 5890 6077 1700
Size: 110 293 3787 187 2869
Fragments arranged by size:
3787 2869 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 3765 4377 96
Size: 3669 612 2965
Fragments arranged by size:
3669 2965 612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7246
BssSI C'ACGA_G
Cuts at: 5306 6318 5306
Size: 1012 6234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2182 794
Size: 1388 5858
BstBI TT'CG_AA
Cuts at: 6050 6050
Size: 7246
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7246
BstXI CCAn_nnnn'nTGG
Cuts at: 3477 4395 3477
Size: 918 6328
BstYI r'GATC_y
Cuts at: 1972 2208 2514 3988 4498 4576 4943 5328 5345
Size: 236 306 1474 510 78 367 385 17
Cuts at: 5345 6797 6883 6895 7241 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1474 1452 510 385 367 346 306
236 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2445 2965 3171 4080 513
Size: 552 939 49 392 520 206 909 3679
Fragments arranged by size:
3679 939 909 552 520 392 206 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6507
Fragments arranged by size:
6507 683 56
BtsI GCAGTG_nn'
Cuts at: 4008 4762 5556 5576 4008
Size: 754 794 20 5678
Fragments arranged by size:
5678 794 754 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7211
DraI TTT'AAA
Cuts at: 4321 4550 4321
Size: 229 7017
DraIII CAC_nnn'GTG
Cuts at: 2182 2197 3142 2182
Size: 15 945 6286
Fragments arranged by size:
6286 945 15
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6253 809
Size: 507 4937 1802
Fragments arranged by size:
4937 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2820 3006
Size: 54 1425 50 275 18 312 678 186
Cuts at: 3006 3852 4693 5582 7211 8
Size: 846 841 889 1629 43
Fragments arranged by size:
1629 1425 889 846 841 678 312 275
186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 1537
Size: 275 2881 4090
Fragments arranged by size:
4090 2881 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2361 3297 3704 4116 4283 5176 1353
Size: 734 274 936 407 412 167 893 3423
Fragments arranged by size:
3423 936 893 734 412 407 274 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 5833 6207 6353 387
Size: 1064 138 1332 2912 374 146 1280
Fragments arranged by size:
2912 1332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3106 3511 3106
Size: 405 6841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 3179 3403 3656 3674 3864 4241
Size: 393 476 1071 224 253 18 190 377
Cuts at: 4241 4426 4479 5309 5906 6693 6961 1239
Size: 185 53 830 597 787 268 1524
Fragments arranged by size:
1524 1071 830 787 597 476 393 377
268 253 224 190 185 53 18
EcoO109I rG'GnC_Cy
Cuts at: 2839 4062 4063 2839
Size: 1223 1 6022
Fragments arranged by size:
6022 1223 1
EcoRI G'AATT_C
Cuts at: 2591 4555 4933 2591
Size: 1964 378 4904
Fragments arranged by size:
4904 1964 378
EcoRV GAT'ATC
Cuts at: 2926 3845 4288 2926
Size: 919 443 5884
Fragments arranged by size:
5884 919 443
FspI TGC'GCA
Cuts at: 5752 5752
Size: 7246
HaeII r_GCGC'y
Cuts at: 1678 2206 3166 3478 6393 1678
Size: 528 960 312 2915 2531
Fragments arranged by size:
2915 2531 960 528 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2491 2523 2644 2676 5887
Size: 32 135 32 919 32 121 32 3211
Cuts at: 5887 5919 5961 5993 1373
Size: 32 42 32 2626
Fragments arranged by size:
3211 2626 919 135 121 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 4590 4836 669
Size: 988 281 784 1868 246 3079
Fragments arranged by size:
3079 1868 988 784 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7246
HpaI GTT'AAC
Cuts at: 4836 4836
Size: 7246
KpnI G_GTAC'C
Cuts at: 4571 4571
Size: 7246
MfeI C'AATT_G
Cuts at: 4845 4845
Size: 7246
MluI A'CGCG_T
Cuts at: 7122 7122
Size: 7246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2764 3895 6360 6544 1305
Size: 1459 1131 2465 184 2007
Fragments arranged by size:
2465 2007 1459 1131 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 3008 3854 10
Size: 54 1425 343 312 864 846 3402
Fragments arranged by size:
3402 1425 864 846 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2613 2663 2693 3881 4295 5204 5563
Size: 648 1447 50 30 1188 414 909 359
Cuts at: 5563 5722 518
Size: 159 2042
Fragments arranged by size:
2042 1447 1188 909 648 414 359 159
50 30
MspA1I CmG'CkG
Cuts at: 1606 2055 3016 3559 4375 4662 5339 6487 6732
Size: 449 961 543 816 287 677 1148 245
Cuts at: 6732 1606
Size: 2120
Fragments arranged by size:
2120 1148 961 816 677 543 449 287
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2445 4080 513
Size: 552 939 441 1635 3679
Fragments arranged by size:
3679 1635 939 552 441
NdeI CA'TA_TG
Cuts at: 387 2683 387
Size: 2296 4950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7246
NotI GC'GGCC_GC
Cuts at: 4693 4693
Size: 7246
NruI TCG'CGA
Cuts at: 3041 3041
Size: 7246
NspI r_CATG'y
Cuts at: 1132 1229 3755 4300 4604 6149 1132
Size: 97 2526 545 304 1545 2229
Fragments arranged by size:
2526 2229 1545 545 304 97
PasI CC'CwG_GG
Cuts at: 1348 2455 1348
Size: 1107 6139
PciI A'CATG_T
Cuts at: 6145 6145
Size: 7246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 3099 1108
Size: 1089 902 5255
Fragments arranged by size:
5255 1089 902
PfoI T'CCnGG_A
Cuts at: 2994 3147 3600 3651 2994
Size: 153 453 51 6589
Fragments arranged by size:
6589 453 153 51
PmeI GTTT'AAAC
Cuts at: 4550 4550
Size: 7246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5315 5347 6828 6860 4115
Size: 32 1168 32 1481 32 4501
Fragments arranged by size:
4501 1481 1168 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2839 2839
Size: 7246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7246
PsiI TTA'TAA
Cuts at: 4816 4816
Size: 7246
PspOMI G'GGCC_C
Cuts at: 4062 4062
Size: 7246
PstI C_TGCA'G
Cuts at: 830 4015 4598 830
Size: 3185 583 3478
Fragments arranged by size:
3478 3185 583
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7246
PvuII CAG'CTG
Cuts at: 3016 3559 4375 3016
Size: 543 816 5887
Fragments arranged by size:
5887 816 543
SacI G_AGCT'C
Cuts at: 3108 3513 3108
Size: 405 6841
SacII CC_GC'GG
Cuts at: 2056 2056
Size: 7246
SalI G'TCGA_C
Cuts at: 1936 4588 1936
Size: 2652 4594
SbfI CC_TGCA'GG
Cuts at: 4598 4598
Size: 7246
ScaI AGT'ACT
Cuts at: 5494 7206 5494
Size: 1712 5534
SexAI A'CCwGG_T
Cuts at: 2193 3177 2193
Size: 984 6262
SfcI C'TryA_G
Cuts at: 826 1045 3949 4011 4594 5729 6410 6601 826
Size: 219 2904 62 583 1135 681 191 1471
Fragments arranged by size:
2904 1471 1135 681 583 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7246
SmaI CCC'GGG
Cuts at: 1872 2837 4573 1872
Size: 965 1736 4545
Fragments arranged by size:
4545 1736 965
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 5350 6251 6513 820
Size: 197 926 718 2689 901 262 1553
Fragments arranged by size:
2689 1553 926 901 718 262 197
SnaBI TAC'GTA
Cuts at: 493 5037 493
Size: 4544 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7246
SphI G_CATG'C
Cuts at: 1132 4604 1132
Size: 3472 3774
SspI AAT'ATT
Cuts at: 5 52 5170 5
Size: 47 5118 2081
Fragments arranged by size:
5118 2081 47
StuI AGG'CCT
Cuts at: 1517 4394 1517
Size: 2877 4369
StyI C'CwwG_G
Cuts at: 513 1065 2004 2445 2570 3093 3470 3612 3806
Size: 552 939 441 125 523 377 142 194
Cuts at: 3806 4080 4685 513
Size: 274 605 3074
Fragments arranged by size:
3074 939 605 552 523 441 377 274
194 142 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2826 3125 3572 4464 5273 5290
Size: 1255 723 523 299 447 892 809 17
Cuts at: 5290 5443 5628 6073 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 892 809 723 523 447 445
299 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3663 3723 3765
Size: 274 80 33 51 1443 1686 60 42
Cuts at: 3765 4377 5492 7204 96
Size: 612 1115 1712 138
Fragments arranged by size:
1712 1686 1443 1115 612 274 138 80
60 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3027 3059 7206 7238 3027
Size: 32 4147 32 3035
Fragments arranged by size:
4147 3035 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7246
XbaI T'CTAG_A
Cuts at: 4582 4582
Size: 7246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2452 3362 4099 2452
Size: 910 737 5599
Fragments arranged by size:
5599 910 737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7246
XmaI C'CCGG_G
Cuts at: 1870 2835 4571 1870
Size: 965 1736 4545
Fragments arranged by size:
4545 1736 965
XmnI GAAnn'nnTTC
Cuts at: 2096 3119 3797 4130 5375 2096
Size: 1023 678 333 1245 3967
Fragments arranged by size:
3967 1245 1023 678 333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5054 276
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SfiI SfoI SrfI SwaI