(Linear) (Six-base) MAPSORT of: KIEE5817.seq Check: 832 from: 1 to: 2541 KIEE5817.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 113 2541 Size: 113 2428 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 1398 1651 1859 2236 2474 2541 Size: 103 1295 253 208 377 238 67 Fragments arranged by size: 1295 377 253 238 208 103 67 AfeI AGC'GCT Cuts at: 0 1159 2541 Size: 1159 1382 AleI CACnn'nnGTG Cuts at: 0 658 688 1876 2541 Size: 658 30 1188 665 Fragments arranged by size: 1188 665 658 30 AlwNI CAG_nnn'CTG Cuts at: 0 973 1647 2541 Size: 973 674 894 Fragments arranged by size: 973 894 674 ApaI G_GGCC'C Cuts at: 0 2061 2541 Size: 2061 480 ApaLI G'TGCA_C Cuts at: 0 601 2541 Size: 601 1940 ApoI r'AATT_y Cuts at: 0 265 586 2431 2541 Size: 265 321 1845 110 Fragments arranged by size: 1845 321 265 110 AvaI C'yCGr_G Cuts at: 0 740 830 2022 2424 2541 Size: 740 90 1192 402 117 Fragments arranged by size: 1192 740 402 117 90 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1702 1735 2541 Size: 1702 33 806 Fragments arranged by size: 1702 806 33 BamHI G'GATC_C Cuts at: 0 203 2541 Size: 203 2338 BanI G'GyrC_C Cuts at: 0 101 744 2011 2541 Size: 101 643 1267 530 Fragments arranged by size: 1267 643 530 101 BanII G_rGCy'C Cuts at: 0 1103 1508 2061 2541 Size: 1103 405 553 480 Fragments arranged by size: 1103 553 480 405 BbsI GAAGACnn'nnnn_ Cuts at: 0 1117 1399 1535 1581 2541 Size: 1117 282 136 46 960 Fragments arranged by size: 1117 960 282 136 46 BciVI GTATCCnnnnn_n' Cuts at: 0 463 1703 2541 Size: 463 1240 838 Fragments arranged by size: 1240 838 463 BclI T'GATC_A Cuts at: 0 299 1751 1856 1868 2229 2541 Size: 299 1452 105 12 361 312 Fragments arranged by size: 1452 361 312 299 105 12 BglII A'GATC_T Cuts at: 0 1983 2493 2541 Size: 1983 510 48 Fragments arranged by size: 1983 510 48 Bme1580I G_kGCm'C Cuts at: 0 238 605 695 747 2061 2541 Size: 238 367 90 52 1314 480 Fragments arranged by size: 1314 480 367 238 90 52 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 843 2541 Size: 843 1698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1174 1669 2421 2541 Size: 1174 495 752 120 Fragments arranged by size: 1174 752 495 120 Bpu10I CC'TnA_GC Cuts at: 0 1043 1546 2541 Size: 1043 503 995 Fragments arranged by size: 1043 995 503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 641 2541 Size: 641 1900 BsaBI GATnn'nnATC Cuts at: 0 43 2541 Size: 43 2498 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1642 1666 1672 1696 2541 Size: 1642 24 6 24 845 Fragments arranged by size: 1642 845 24 24 6 BseYI C'CCAG_C Cuts at: 0 2110 2541 Size: 2110 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 986 1013 2541 Size: 91 895 27 1528 Fragments arranged by size: 1528 895 91 27 BsiHKAI G_wGCw'C Cuts at: 0 605 1103 1142 1508 2541 Size: 605 498 39 366 1033 Fragments arranged by size: 1033 605 498 366 39 BsmI GAATG_Cn' Cuts at: 0 476 2541 Size: 476 2065 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1645 2541 Size: 1645 896 Bsp1286I G_dGCh'C Cuts at: 0 238 605 695 747 1103 1142 1508 2061 Size: 238 367 90 52 356 39 366 553 Cuts at: 2061 2541 Size: 480 Fragments arranged by size: 553 480 367 366 356 238 90 52 39 BspHI T'CATG_A Cuts at: 0 2304 2541 Size: 2304 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 113 516 2541 Size: 113 403 2025 Fragments arranged by size: 2025 403 113 BsrDI GCAATG_nn' Cuts at: 0 405 2541 Size: 405 2136 BsrFI r'CCGG_y Cuts at: 0 98 2541 Size: 98 2443 BsrGI T'GTAC_A Cuts at: 0 1760 2372 2541 Size: 1760 612 169 Fragments arranged by size: 1760 612 169 BstAPI GCAn_nnn'nTGC Cuts at: 0 177 2541 Size: 177 2364 BstXI CCAn_nnnn'nTGG Cuts at: 0 1472 2390 2541 Size: 1472 918 151 Fragments arranged by size: 1472 918 151 BstYI r'GATC_y Cuts at: 0 203 509 1983 2493 2541 Size: 203 306 1474 510 48 Fragments arranged by size: 1474 510 306 203 48 BtgI C'CryG_G Cuts at: 0 48 440 960 1166 2075 2541 Size: 48 392 520 206 909 466 Fragments arranged by size: 909 520 466 392 206 48 BtsI GCAGTG_nn' Cuts at: 0 2003 2541 Size: 2003 538 DraI TTT'AAA Cuts at: 0 2316 2541 Size: 2316 225 DraIII CAC_nnn'GTG Cuts at: 0 177 192 1137 2541 Size: 177 15 945 1404 Fragments arranged by size: 1404 945 177 15 EaeI y'GGCC_r Cuts at: 0 137 815 1001 1847 2541 Size: 137 678 186 846 694 Fragments arranged by size: 846 694 678 186 137 EarI CTCTTCn'nnn_ Cuts at: 0 82 356 1292 1699 2111 2278 2541 Size: 82 274 936 407 412 167 263 Fragments arranged by size: 936 412 407 274 263 167 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 916 2541 Size: 916 1625 EcoICRI GAG'CTC Cuts at: 0 1101 1506 2541 Size: 1101 405 1035 Fragments arranged by size: 1101 1035 405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 1174 1398 1651 1669 1859 2236 2421 Size: 103 1071 224 253 18 190 377 185 Cuts at: 2421 2474 2541 Size: 53 67 Fragments arranged by size: 1071 377 253 224 190 185 103 67 53 18 EcoO109I rG'GnC_Cy Cuts at: 0 834 2057 2058 2541 Size: 834 1223 1 483 Fragments arranged by size: 1223 834 483 1 EcoRI G'AATT_C Cuts at: 0 586 2541 Size: 586 1955 EcoRV GAT'ATC Cuts at: 0 921 1840 2283 2541 Size: 921 919 443 258 Fragments arranged by size: 921 919 443 258 HaeII r_GCGC'y Cuts at: 0 201 1161 1473 2541 Size: 201 960 312 1068 Fragments arranged by size: 1068 960 312 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 486 518 639 671 2541 Size: 486 32 121 32 1870 Fragments arranged by size: 1870 486 121 32 32 HincII GTy'rAC Cuts at: 0 717 2541 Size: 717 1824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 759 1890 2541 Size: 759 1131 651 Fragments arranged by size: 1131 759 651 MscI TGG'CCA Cuts at: 0 139 1003 1849 2541 Size: 139 864 846 692 Fragments arranged by size: 864 846 692 139 MslI CAynn'nnrTG Cuts at: 0 608 658 688 1876 2290 2541 Size: 608 50 30 1188 414 251 Fragments arranged by size: 1188 608 414 251 50 30 MspA1I CmG'CkG Cuts at: 0 50 1011 1554 2370 2541 Size: 50 961 543 816 171 Fragments arranged by size: 961 816 543 171 50 NcoI C'CATG_G Cuts at: 0 440 2075 2541 Size: 440 1635 466 Fragments arranged by size: 1635 466 440 NdeI CA'TA_TG Cuts at: 0 678 2541 Size: 678 1863 NruI TCG'CGA Cuts at: 0 1036 2541 Size: 1036 1505 NspI r_CATG'y Cuts at: 0 1750 2295 2541 Size: 1750 545 246 Fragments arranged by size: 1750 545 246 PasI CC'CwG_GG Cuts at: 0 450 2541 Size: 450 2091 PflMI CCAn_nnn'nTGG Cuts at: 0 192 1094 2541 Size: 192 902 1447 Fragments arranged by size: 1447 902 192 PfoI T'CCnGG_A Cuts at: 0 989 1142 1595 1646 2541 Size: 989 153 453 51 895 Fragments arranged by size: 989 895 453 153 51 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2110 2142 2541 Size: 2110 32 399 Fragments arranged by size: 2110 399 32 PpuMI rG'GwC_Cy Cuts at: 0 834 2541 Size: 834 1707 PspOMI G'GGCC_C Cuts at: 0 2057 2541 Size: 2057 484 PstI C_TGCA'G Cuts at: 0 2010 2541 Size: 2010 531 PvuII CAG'CTG Cuts at: 0 1011 1554 2370 2541 Size: 1011 543 816 171 Fragments arranged by size: 1011 816 543 171 SacI G_AGCT'C Cuts at: 0 1103 1508 2541 Size: 1103 405 1033 Fragments arranged by size: 1103 1033 405 SacII CC_GC'GG Cuts at: 0 51 2541 Size: 51 2490 SexAI A'CCwGG_T Cuts at: 0 188 1172 2541 Size: 188 984 1369 Fragments arranged by size: 1369 984 188 SfcI C'TryA_G Cuts at: 0 1944 2006 2541 Size: 1944 62 535 Fragments arranged by size: 1944 535 62 SmaI CCC'GGG Cuts at: 0 832 2541 Size: 832 1709 SmlI C'TyrA_G Cuts at: 0 656 2541 Size: 656 1885 StuI AGG'CCT Cuts at: 0 2389 2541 Size: 2389 152 StyI C'CwwG_G Cuts at: 0 440 565 1088 1465 1607 1801 2075 2541 Size: 440 125 523 377 142 194 274 466 Fragments arranged by size: 523 466 440 377 274 194 142 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 298 821 1120 1567 2459 2541 Size: 298 523 299 447 892 82 Fragments arranged by size: 892 523 447 299 298 82 TatI w'GTAC_w Cuts at: 0 1658 1718 1760 2372 2541 Size: 1658 60 42 612 169 Fragments arranged by size: 1658 612 169 60 42 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1022 1054 2541 Size: 1022 32 1487 Fragments arranged by size: 1487 1022 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 447 1357 2094 2541 Size: 447 910 737 447 Fragments arranged by size: 910 737 447 447 XmaI C'CCGG_G Cuts at: 0 830 2541 Size: 830 1711 XmnI GAAnn'nnTTC Cuts at: 0 91 1114 1792 2125 2541 Size: 91 1023 678 333 416 Fragments arranged by size: 1023 678 416 333 91 Enzymes that do cut: AarI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BciVI BclI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaBI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI NruI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EcoNI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI