################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MboII MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 62 AAATTT [GA]^AATT[CT] AcyI 316 GACGCC G[GA]^CG[CT]C ApoI 62 AAATTT [GA]^AATT[CT] Asp700I 59 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 240 TGGCCA TGG^CCA BclI 351 TGATCA T^GATCA BpmI 111 CTCCAG CTGGAG(16/14) BsaBI 157 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 316 GACGCC G[GA]^CG[CT]C BsaI 12 GGTCTC GGTCTC(1/5) BsgI 35 GTGCAG GTGCAG(16/14) BsiEI 311 CGATCG CG[GA][CT]^CG BsmAI 13 GTCTC GTCTC(1/5) BsmI 135 GAATGC GAATGC(1/-1) BspHI 335 TCATGA T^CATGA BssKI 243 CCAGG ^CC[ACGT]GG BstNI 243 CCAGG CC^[AT]GG DdeI 17 CTGAG C^T[ACGT]AG DraII 282 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 240 TGGCCA [CT]^GGCC[GA] EcoO109I 282 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 243 CCAGG ^CC[AT]GG FbaI 351 TGATCA T^GATCA HgaI 316 GACGC GACGC(5/10) Hin1I 316 GACGCC G[GA]^CG[CT]C HphI 29 TCACC GGTGA(8/7) MaeIII 107 GTGAC ^GT[ACGT]AC MamI 157 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 240 TGGCCA TGG^CCA MscI 240 TGGCCA TGG^CCA MseI 146 TTAA T^TAA MvaI 243 CCAGG CC^[AT]GG NlaIV 220 GGGGCC GG[ACGT]^[ACGT]CC PvuI 311 CGATCG CGAT^CG RcaI 335 TCATGA T^CATGA ScrFI 243 CCAGG CC^[ACGT]GG SspI 370 AATATT AAT^ATT TaqI 314 TCGA T^CGA TfiI 303 GATTC G^A[AT]TC Tru9I 146 TTAA T^TAA Tsp45I 107 GTGAC ^GT[GC]AC Tsp509I 63 AATT ^AATT TspRI 140 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 314 TCGA T^CGA XcmI 69 CCAGCAGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 59 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 10 GCGG CCGC(-3/-1) 193 GCGG BbvI 21 GCAGC GCAGC(8/12) 210 GCTGC BsiYI 183 CCCTGATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCATGGCCAGG BslI 183 CCCTGATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCATGGCCAGG BstF5I 197 CATCC GGATG(2/0) 174 GGATG CfoI 213 GCGC GCG^C 231 GCGC FokI 197 CATCC GGATG(9/13) 174 GGATG HhaI 213 GCGC GCG^C 231 GCGC HinP1I 213 GCGC G^CGC 231 GCGC MslI 98 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 197 CATCCGAGTG MwoI 233 GCTACCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 310 GCGATCGACGC TseI 21 GCAGC G^C[AT]GC 210 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### BsaJI Cfr13I DpnII DsaI EcoT14I Fnu4HI HaeIII HinfI ItaI MboI MnlI NcoI NdeII NlaIII PleI Sau3AI Sau96I SfaNI StyI