(Circular) (Six-base) MAPSORT of: pFN21AE6180.seq Check: 5036 from: 1 to: 5086 pFN21AE6180 5086 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2896 278 Size: 53 83 186 936 1360 2468 Fragments arranged by size: 2468 1360 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2429 1937 Size: 492 4594 Acc65I G'GTAC_C Cuts at: 2407 2407 Size: 5086 AclI AA'CG_TT Cuts at: 1563 3213 3586 1563 Size: 1650 373 3063 Fragments arranged by size: 3063 1650 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3149 4533 4801 1632 Size: 1517 1384 268 1917 Fragments arranged by size: 1917 1517 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4889 AflIII A'CryG_T Cuts at: 1203 3985 4962 1203 Size: 2782 977 1327 Fragments arranged by size: 2782 1327 977 AgeI A'CCGG_T Cuts at: 3917 3917 Size: 5086 AhdI GACnn_n'nnGTC Cuts at: 3815 3815 Size: 5086 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5086 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5086 AlwNI CAG_nnn'CTG Cuts at: 2829 4401 2829 Size: 1572 3514 ApaLI G'TGCA_C Cuts at: 3143 4299 3143 Size: 1156 3930 ApoI r'AATT_y Cuts at: 1493 2067 2395 2621 2773 4882 1493 Size: 574 328 226 152 2109 1697 Fragments arranged by size: 2109 1697 574 328 226 152 AseI AT'TA_AT Cuts at: 160 1032 3640 160 Size: 872 2608 1606 Fragments arranged by size: 2608 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5086 AvaI C'yCGr_G Cuts at: 1870 1943 2411 1870 Size: 73 468 4545 Fragments arranged by size: 4545 468 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2320 2353 2320 Size: 33 5053 BamHI G'GATC_C Cuts at: 2416 2783 2416 Size: 367 4719 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 3862 618 Size: 325 217 627 620 1455 1842 Fragments arranged by size: 1842 1455 627 620 325 217 BanII G_rGCy'C Cuts at: 1391 2405 1391 Size: 1014 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3277 3311 1903 Size: 34 1340 34 3678 Fragments arranged by size: 3678 1340 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2978 4188 2978 Size: 1210 3876 BclI T'GATC_A Cuts at: 1548 2356 1548 Size: 808 4278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3697 136 Size: 107 122 71 631 605 2025 1525 Fragments arranged by size: 2025 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5081 5081 Size: 5086 BlpI GC'TnA_GC Cuts at: 2503 2503 Size: 5086 Bme1580I G_kGCm'C Cuts at: 1790 3147 4303 1790 Size: 1357 1156 2573 Fragments arranged by size: 2573 1357 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2817 3775 442 Size: 416 53 83 399 24 1400 958 1753 Fragments arranged by size: 1753 1400 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5086 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 3746 1239 Size: 862 1645 2579 Fragments arranged by size: 2579 1645 862 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3211 4076 4374 3211 Size: 865 298 3923 Fragments arranged by size: 3923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 3749 882 Size: 617 524 1726 2219 Fragments arranged by size: 2219 1726 617 524 BsaAI yAC'GTr Cuts at: 493 1204 2877 493 Size: 711 1673 2702 Fragments arranged by size: 2702 1673 711 BsaBI GATnn'nnATC Cuts at: 2166 5080 2166 Size: 2914 2172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 2893 3275 3910 Size: 53 83 186 936 789 571 382 635 Cuts at: 3910 275 Size: 1451 Fragments arranged by size: 1451 936 789 635 571 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3519 3917 4191 4338 4749 3519 Size: 398 274 147 411 3856 Fragments arranged by size: 3856 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4861 Fragments arranged by size: 4861 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5086 BseYI C'CCAG_C Cuts at: 2073 4289 4785 2073 Size: 2216 496 2374 Fragments arranged by size: 2374 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 1163 Size: 573 325 4188 Fragments arranged by size: 4188 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2319 2536 3297 4325 1540 Size: 275 186 318 217 761 1028 2301 Fragments arranged by size: 2301 1028 761 318 275 217 186 BsiHKAI G_wGCw'C Cuts at: 3147 3232 4303 3147 Size: 85 1071 3930 Fragments arranged by size: 3930 1071 85 BsmI GAATG_Cn' Cuts at: 2146 2597 2690 2146 Size: 451 93 4542 Fragments arranged by size: 4542 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 3147 3232 4303 1391 Size: 399 615 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 399 85 BspEI T'CCGG_A Cuts at: 4749 4749 Size: 5086 BspHI T'CATG_A Cuts at: 2340 2975 4705 2340 Size: 635 1730 2721 Fragments arranged by size: 2721 1730 635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2441 844 Size: 1597 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2973 1394 Size: 429 1150 3507 Fragments arranged by size: 3507 1150 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3581 3755 65 Size: 1183 2333 174 1396 Fragments arranged by size: 2333 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3730 3917 1700 Size: 110 1920 187 2869 Fragments arranged by size: 2869 1920 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5086 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5086 BssSI C'ACGA_G Cuts at: 3146 4158 3146 Size: 1012 4074 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5086 BstBI TT'CG_AA Cuts at: 3890 3890 Size: 5086 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5086 BstYI r'GATC_y Cuts at: 1972 2416 2783 3168 3185 4637 4723 4735 5081 Size: 444 367 385 17 1452 86 12 346 Cuts at: 5081 1972 Size: 1977 Fragments arranged by size: 1977 1452 444 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2102 2229 2242 513 Size: 552 1037 127 13 3357 Fragments arranged by size: 3357 1037 552 127 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4347 Fragments arranged by size: 4347 683 56 BtsI GCAGTG_nn' Cuts at: 2151 2602 3396 3416 2151 Size: 451 794 20 3821 Fragments arranged by size: 3821 794 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5086 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5051 DraI TTT'AAA Cuts at: 2390 2390 Size: 5086 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4093 809 Size: 507 2777 1802 Fragments arranged by size: 2777 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2533 3422 Size: 54 1425 50 275 18 415 288 889 Cuts at: 3422 5051 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 415 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2533 1537 Size: 275 721 4090 Fragments arranged by size: 4090 721 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3016 1353 Size: 1663 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3673 4047 4193 387 Size: 1064 138 2084 374 146 1280 Fragments arranged by size: 2084 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 3149 3746 4533 4801 1239 Size: 393 469 1048 597 787 268 1524 Fragments arranged by size: 1524 1048 787 597 469 393 268 EcoO109I rG'GnC_Cy Cuts at: 2288 2288 Size: 5086 EcoRI G'AATT_C Cuts at: 2395 2773 2395 Size: 378 4708 FspI TGC'GCA Cuts at: 3592 3592 Size: 5086 HaeII r_GCGC'y Cuts at: 1678 4233 1678 Size: 2555 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3727 3759 3801 3833 1373 Size: 32 135 32 2155 32 42 32 2626 Fragments arranged by size: 2626 2155 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2430 2676 669 Size: 988 281 492 246 3079 Fragments arranged by size: 3079 988 492 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5086 HpaI GTT'AAC Cuts at: 2676 2676 Size: 5086 KpnI G_GTAC'C Cuts at: 2411 2411 Size: 5086 MfeI C'AATT_G Cuts at: 2685 2685 Size: 5086 MluI A'CGCG_T Cuts at: 4962 4962 Size: 5086 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2306 4200 4384 1305 Size: 1001 1894 184 2007 Fragments arranged by size: 2007 1894 1001 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2247 10 Size: 54 1425 343 415 2849 Fragments arranged by size: 2849 1425 415 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2107 2206 3044 3403 3562 518 Size: 648 941 99 838 359 159 2042 Fragments arranged by size: 2042 941 838 648 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2502 3179 4327 4572 1606 Size: 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 245 NcoI C'CATG_G Cuts at: 513 1065 2102 2229 2242 513 Size: 552 1037 127 13 3357 Fragments arranged by size: 3357 1037 552 127 13 NdeI CA'TA_TG Cuts at: 387 387 Size: 5086 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5086 NotI GC'GGCC_GC Cuts at: 2533 2533 Size: 5086 NspI r_CATG'y Cuts at: 1132 1229 2444 3989 1132 Size: 97 1215 1545 2229 Fragments arranged by size: 2229 1545 1215 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5086 PciI A'CATG_T Cuts at: 3985 3985 Size: 5086 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5086 PmeI GTTT'AAAC Cuts at: 2390 2390 Size: 5086 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 4668 4700 3155 Size: 32 1481 32 3541 Fragments arranged by size: 3541 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5086 PsiI TTA'TAA Cuts at: 2656 2656 Size: 5086 PstI C_TGCA'G Cuts at: 830 2438 830 Size: 1608 3478 PvuI CG_AT'CG Cuts at: 2001 2319 2001 Size: 318 4768 SalI G'TCGA_C Cuts at: 1936 2428 1936 Size: 492 4594 SbfI CC_TGCA'GG Cuts at: 2438 2438 Size: 5086 ScaI AGT'ACT Cuts at: 3334 5046 3334 Size: 1712 3374 SfcI C'TryA_G Cuts at: 826 1045 2434 3569 4250 4441 826 Size: 219 1389 1135 681 191 1471 Fragments arranged by size: 1471 1389 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5086 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5086 SmaI CCC'GGG Cuts at: 1872 2413 1872 Size: 541 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4091 4353 820 Size: 197 926 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2877 493 Size: 2384 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5086 SphI G_CATG'C Cuts at: 1132 2444 1132 Size: 1312 3774 SspI AAT'ATT Cuts at: 5 52 2377 3010 5 Size: 47 2325 633 2081 Fragments arranged by size: 2325 2081 633 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5086 StyI C'CwwG_G Cuts at: 513 1065 2102 2229 2242 2525 513 Size: 552 1037 127 13 283 3074 Fragments arranged by size: 3074 1037 552 283 127 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3130 3283 3468 3913 325 Size: 1255 1533 17 153 185 445 1498 Fragments arranged by size: 1533 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3332 5044 96 Size: 274 80 33 51 1443 1355 1712 138 Fragments arranged by size: 1712 1443 1355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5046 5078 5046 Size: 32 5054 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5086 XbaI T'CTAG_A Cuts at: 2422 2422 Size: 5086 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2081 2081 Size: 5086 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5086 XmaI C'CCGG_G Cuts at: 1870 2411 1870 Size: 541 4545 XmnI GAAnn'nnTTC Cuts at: 2068 3215 2068 Size: 1147 3939 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2894 276 Size: 53 83 186 936 1360 2468 Fragments arranged by size: 2468 1360 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI