(Circular) (Six-base) MAPSORT of: pFN21AB9939.seq Check: 9403 from: 1 to: 6286 pFN21AB9939 6286 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3181 3607 3181 Size: 426 5860 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 4349 5733 6001 1632 Size: 455 2262 1384 268 1917 Fragments arranged by size: 2262 1917 1384 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AlwNI CAG_nnn'CTG Cuts at: 3282 4029 5601 3282 Size: 747 1572 3967 Fragments arranged by size: 3967 1572 747 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2318 2356 2489 2585 3167 3216 3595 3821 Size: 825 38 133 96 582 49 379 226 Cuts at: 3821 3973 6082 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 825 582 379 226 152 133 96 49 38 AseI AT'TA_AT Cuts at: 160 1032 2151 2778 4840 160 Size: 872 1119 627 2062 1606 Fragments arranged by size: 2062 1606 1119 872 627 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BamHI G'GATC_C Cuts at: 3109 3616 3983 3109 Size: 507 367 5412 Fragments arranged by size: 5412 507 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3181 3607 5062 618 Size: 325 217 627 221 1173 426 1455 1842 Fragments arranged by size: 1842 1455 1173 627 426 325 221 217 BanII G_rGCy'C Cuts at: 1391 2745 3605 1391 Size: 1354 860 4072 Fragments arranged by size: 4072 1354 860 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2819 3238 3261 928 Size: 971 920 419 23 3953 Fragments arranged by size: 3953 971 920 419 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3429 4178 5388 3429 Size: 749 1210 4327 Fragments arranged by size: 4327 1210 749 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 4897 136 Size: 107 122 71 631 605 345 2880 1525 Fragments arranged by size: 2880 1525 631 605 345 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 3429 4347 5503 1790 Size: 1639 918 1156 2573 Fragments arranged by size: 2573 1639 1156 918 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3377 4017 4975 Size: 416 53 83 399 24 1960 640 958 Cuts at: 4975 442 Size: 1753 Fragments arranged by size: 1960 1753 958 640 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4946 1239 Size: 3707 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4949 882 Size: 617 3450 2219 Fragments arranged by size: 3450 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4093 4475 5110 Size: 53 83 186 936 476 2084 382 635 Cuts at: 5110 275 Size: 1451 Fragments arranged by size: 2084 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2452 4719 5117 5391 5538 5949 2452 Size: 2267 398 274 147 411 2789 Fragments arranged by size: 2789 2267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2439 2858 1186 Size: 1253 419 4614 Fragments arranged by size: 4614 1253 419 BseYI C'CCAG_C Cuts at: 5489 5985 5489 Size: 496 5790 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2334 2970 3295 1163 Size: 573 598 636 325 4154 Fragments arranged by size: 4154 636 598 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 4347 4432 5503 2256 Size: 2091 85 1071 3039 Fragments arranged by size: 3039 2091 1071 85 BsmI GAATG_Cn' Cuts at: 2228 3488 3797 3890 2228 Size: 1260 309 93 4624 Fragments arranged by size: 4624 1260 309 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2745 3429 3605 4347 4432 5503 Size: 399 466 489 684 176 742 85 1071 Cuts at: 5503 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 684 489 466 399 176 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 3641 844 Size: 2119 678 3489 Fragments arranged by size: 3489 2119 678 BsrBI CCG'CTC Cuts at: 1394 1823 2017 4173 1394 Size: 429 194 2156 3507 Fragments arranged by size: 3507 2156 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 3441 4781 4955 65 Size: 1183 2193 1340 174 1396 Fragments arranged by size: 2193 1396 1340 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 3227 96 Size: 2202 929 3155 Fragments arranged by size: 3155 2202 929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 3282 794 Size: 2488 3798 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstXI CCAn_nnnn'nTGG Cuts at: 3390 3390 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2599 3109 3616 3983 4368 4385 5837 5923 Size: 627 510 507 367 385 17 1452 86 Cuts at: 5923 5935 6281 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 627 510 507 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 513 Size: 552 939 64 4731 Fragments arranged by size: 4731 939 552 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 3802 4596 4616 3802 Size: 794 20 5472 Fragments arranged by size: 5472 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251 Size: 54 1425 50 275 18 1903 889 1629 Cuts at: 6251 8 Size: 43 Fragments arranged by size: 1903 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2835 4216 1353 Size: 753 729 1381 3423 Fragments arranged by size: 3423 1381 753 729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2029 4873 5247 5393 387 Size: 1064 138 440 2844 374 146 1280 Fragments arranged by size: 2844 1280 1064 440 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 4349 4946 5733 6001 1239 Size: 393 455 2262 597 787 268 1524 Fragments arranged by size: 2262 1524 787 597 455 393 268 EcoO109I rG'GnC_Cy Cuts at: 2424 2792 2424 Size: 368 5918 EcoRI G'AATT_C Cuts at: 3167 3216 3595 3973 3167 Size: 49 379 378 5480 Fragments arranged by size: 5480 379 378 49 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 3355 2188 Size: 1167 5119 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 2012 2036 5433 1678 Size: 334 24 3397 2531 Fragments arranged by size: 3397 2531 334 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2449 2481 3403 3435 4927 Size: 32 135 32 877 32 922 32 1492 Cuts at: 4927 4959 5001 5033 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1492 922 877 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 3012 748 Size: 2264 4022 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6286 KpnI G_GTAC'C Cuts at: 3185 3611 3185 Size: 426 5860 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 2868 2975 5400 5584 1305 Size: 1336 227 107 2425 184 2007 Fragments arranged by size: 2425 2007 1336 227 184 107 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4464 Fragments arranged by size: 4464 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 2472 2715 4244 4603 4762 518 Size: 648 1267 39 243 1529 359 159 2042 Fragments arranged by size: 2042 1529 1267 648 359 243 159 39 MspA1I CmG'CkG Cuts at: 1606 3702 4379 5527 5772 1606 Size: 2096 677 1148 245 2120 Fragments arranged by size: 2120 2096 1148 677 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6286 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4795 Fragments arranged by size: 4795 939 552 NdeI CA'TA_TG Cuts at: 387 2992 3397 387 Size: 2605 405 3276 Fragments arranged by size: 3276 2605 405 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2670 3644 5189 1132 Size: 97 1441 974 1545 2229 Fragments arranged by size: 2229 1545 1441 974 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 3518 1108 Size: 2410 3876 PfoI T'CCnGG_A Cuts at: 2217 3112 2217 Size: 895 5391 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2424 2792 2424 Size: 368 5918 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SapI GCTCTTCn'nnn_ Cuts at: 2106 2106 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 2752 3224 4534 6246 2752 Size: 472 1310 1712 2792 Fragments arranged by size: 2792 1712 1310 472 SexAI A'CCwGG_T Cuts at: 2892 3097 2892 Size: 205 6081 SfcI C'TryA_G Cuts at: 826 1045 2247 3634 4769 5450 5641 826 Size: 219 1202 1387 1135 681 191 1471 Fragments arranged by size: 1471 1387 1202 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6286 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4390 5291 5553 820 Size: 197 926 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 2670 3644 1132 Size: 1538 974 3774 Fragments arranged by size: 3774 1538 974 SspI AAT'ATT Cuts at: 5 52 2776 4210 5 Size: 47 2724 1434 2081 Fragments arranged by size: 2724 2081 1434 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 2004 3134 3346 3725 513 Size: 552 939 1130 212 379 3074 Fragments arranged by size: 3074 1130 939 552 379 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2749 4313 4330 4483 4668 5113 325 Size: 1255 1169 1564 17 153 185 445 1498 Fragments arranged by size: 1564 1498 1255 1169 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 2525 2750 Size: 274 80 33 51 1443 321 227 225 Cuts at: 2750 3222 3227 4532 6244 96 Size: 472 5 1305 1712 138 Fragments arranged by size: 1712 1443 1305 472 321 274 227 225 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 2100 2913 4415 2100 Size: 813 1502 3971 Fragments arranged by size: 3971 1502 813 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI