################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AciI 313 CCGC CCGC(-3/-1) AfaI 347 GTAC GT^AC Asp700I 947 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 14 CCCGAG C^[CT]CG[GA]G AvaII 622 GGTCC G^G[AT]CC BamHI 579 GGATCC G^GATCC BbvI 83 GCAGC GCAGC(8/12) BsaAI 806 TACGTG [CT]AC^GT[GA] BseRI 484 GAGGAG GAGGAG(10/8) BsgI 751 CTGCAC GTGCAG(16/14) BsoBI 14 CCCGAG C^[CT]CG[GA]G BsrDI 688 GCAATG GCAATG(2/0) DraIII 709 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Fnu4HI 83 GCAGC GC^[ACGT]GC HphI 793 GGTGA GGTGA(8/7) ItaI 83 GCAGC GC^[ACGT]GC MaeII 807 ACGT A^CGT MslI 40 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 313 CCGCTG C[AC]G^C[GT]G MwoI 286 GCTGGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 877 CATG CATG^ RsaI 347 GTAC GT^AC SfaNI 262 GATGC GCATC(5/9) SspI 950 AATATT AAT^ATT StuI 898 AGGCCT AGG^CCT TseI 83 GCAGC G^C[AT]GC XmnI 947 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 341 AAATTC [GA]^AATT[CT] 413 AAATTC AlwI 580 GATCC GGATC(4/5) 579 GGATC ApoI 341 AAATTC [GA]^AATT[CT] 413 AAATTC AspHI 108 GAGCTC G[AT]GC[AT]^C 373 GAGCTC BanII 108 GAGCTC G[GA]GC[CT]^C 373 GAGCTC BfaI 481 CTAG C^TAG 761 CTAG BglII 648 AGATCT A^GATCT 763 AGATCT BmyI 108 GAGCTC G[AGT]GC[ACT]^C 373 GAGCTC BsaI 31 GAGACC GGTCTC(1/5) 117 GGTCTC BsiHKAI 108 GAGCTC G[AT]GC[AT]^C 373 GAGCTC BsmAI 31 GAGAC GTCTC(1/5) 118 GTCTC Bsp1286I 108 GAGCTC G[AGT]GC[ACT]^C 373 GAGCTC Cac8I 315 GCTGGC GC[ACGT]^[ACGT]GC 428 GCCAGC Cfr13I 622 GGTCC G^G[ACGT]CC 703 GGGCC EarI 505 GAAGAG CTCTTC(1/4) 769 GAAGAG HaeIII 704 GGCC GG^CC 899 GGCC HindIII 419 AAGCTT A^AGCTT 919 AAGCTT HinfI 362 GAATC G^A[ACGT]TC 385 GAATC Ksp632I 505 GAAGAG CTCTTC(1/4) 769 GAAGAG MaeI 481 CTAG C^TAG 761 CTAG SacI 108 GAGCTC GAGCT^C 373 GAGCTC Sau96I 622 GGTCC G^G[ACGT]CC 703 GGGCC SfcI 366 CTATAG C^T[GA][CT]AG 937 CTACAG TfiI 362 GAATC G^A[AT]TC 385 GAATC TspRI 102 CACTG CA[GC]TG[ACGT][ACGT]^ 756 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI BbsI BpuAI BsaJI BsrI BstF5I BstYI DdeI DpnII FokI MboI MboII MflI MnlI MseI NdeII NlaIV Sau3AI Tru9I Tsp509I XhoII