Restriction map for
pFN21AE2216
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BanI
BbeI BbsI BciVI BcnI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MscI MseI MslI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NsiI NspI
NspV PacI PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tru9I Tth111I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 140 TCCGGA T^CCGGA
AcsI 584 GAATTT [GA]^AATT[CT]
AhdI 71 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 584 GAATTT [GA]^AATT[CT]
AspEI 71 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 529 GAGCTC G[AT]GC[AT]^C
AvaI 428 CTCGAG C^[CT]CG[GA]G
BbrPI 288 CACGTG CAC^GTG
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
BcgI 420 GCAAAACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 123 TGATCA T^GATCA
BlpI 619 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 619 GCTGAGC GC^T[ACGT]AGC
BsaAI 288 CACGTG [CT]AC^GT[GA]
BsaWI 140 TCCGGA [AT]^CCGG[AT]
BseAI 140 TCCGGA T^CCGGA
BseRI 232 GAGGAG GAGGAG(10/8)
BsiHKAI 529 GAGCTC G[AT]GC[AT]^C
BsmFI 360 GGGAC GGGAC(10/14)
BsoBI 428 CTCGAG C^[CT]CG[GA]G
Bsp1407I 413 TGTACA T^GTACA
BspEI 140 TCCGGA T^CCGGA
BsrDI 390 CATTGC GCAATG(2/0)
BsrGI 413 TGTACA T^GTACA
BsrI 26 CCAGT ACTGG(1/-1)
BstXI 182 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 177 GCTAGC GC[ACGT]^[ACGT]GC
CelII 619 GCTGAGC GC^T[ACGT]AGC
DpnII 124 GATC ^GATC
Eam1105I 71 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 164 CCCGC CCCGC(4/6)
FbaI 123 TGATCA T^GATCA
HapII 141 CCGG C^CGG
HinfI 398 GATTC G^A[ACGT]TC
HpaII 141 CCGG C^CGG
HphI 401 TCACC GGTGA(8/7)
MboI 124 GATC ^GATC
MboII 266 TCTTC GAAGA(8/7)
MroI 140 TCCGGA T^CCGGA
MspI 141 CCGG C^CGG
MwoI 624 GCAGTGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 124 GATC ^GATC
NheI 177 GCTAGC G^CTAGC
NlaIII 604 CATG CATG^
NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC
PaeR7I 428 CTCGAG C^TCGAG
PflMI 270 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 288 CACGTG CAC^GTG
PmlI 288 CACGTG CAC^GTG
PstI 245 CTGCAG CTGCA^G
SacI 529 GAGCTC GAGCT^C
Sau3AI 124 GATC ^GATC
ScaI 519 AGTACT AGT^ACT
SfcI 245 CTGCAG C^T[GA][CT]AG
SmlI 428 CTCGAG C^T[CT][GA]AG
SspBI 413 TGTACA T^GTACA
TaqI 429 TCGA T^CGA
TfiI 398 GATTC G^A[AT]TC
Tsp509I 585 AATT ^AATT
TthHB8I 429 TCGA T^CGA
Van91I 270 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 428 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 287 ACACGT A^C[GA][CT]GT
410 ACGTGT
AlwNI 169 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
271 CAGCTGCTG
BfaI 178 CTAG C^TAG
229 CTAG
BpmI 277 CTGGAG CTGGAG(16/14)
321 CTGGAG
BsaI 193 GAGACC GGTCTC(1/5)
431 GAGACC
BsiYI 270 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCTGGGACGGG
BslI 270 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCTGGGACGGG
BsmAI 193 GAGAC GTCTC(1/5)
431 GAGAC
MaeI 178 CTAG C^TAG
229 CTAG
MaeII 289 ACGT A^CGT
410 ACGT
MaeIII 29 GTGAC ^GT[ACGT]AC
491 GTGAC
MspA1I 155 CAGCTG C[AC]G^C[GT]G
271 CAGCTG
NdeI 350 CATATG CA^TATG
571 CATATG
PvuII 155 CAGCTG CAG^CTG
271 CAGCTG
StuI 147 AGGCCT AGG^CCT
561 AGGCCT
Tsp45I 29 GTGAC ^GT[GC]AC
491 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BsaJI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII ItaI MnlI
MvaI RsaI Sau96I ScrFI TseI
TspRI