################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BalI BanI BbeI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FseI HaeII HapII HgaI HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1144 GACGTC GACGT^C AcyI 1144 GACGTC G[GA]^CG[CT]C Asp700I 784 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 15 GAGCTC G[AT]GC[AT]^C AspI 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1220 GGACC G^G[AT]CC AviII 1241 TGCGCA TGC^GCA BamHI 1658 GGATCC G^GATCC BbrPI 9 CACGTG CAC^GTG BbsI 1374 GAAGAC GAAGAC(2/6) BbvCI 1513 GCTGAGG CCTCAGC(-5/-2) BciVI 969 GGATAC GTATCC(6/5) BpmI 667 CTGGAG CTGGAG(16/14) BpuAI 1374 GAAGAC GAAGAC(2/6) BsaAI 9 CACGTG [CT]AC^GT[GA] BsaHI 1144 GACGTC G[GA]^CG[CT]C BsaI 400 GGTCTC GGTCTC(1/5) BseRI 844 GAGGAG GAGGAG(10/8) BsiHKAI 15 GAGCTC G[AT]GC[AT]^C BspLU11I 955 ACATGT A^CATGT BspMI 973 ACCTGC ACCTGC(4/8) BsrDI 275 CATTGC GCAATG(2/0) CfoI 1242 GCGC GCG^C EcoRI 820 GAATTC G^AATTC EcoRV 1129 GATATC GAT^ATC FauI 54 GCGGG CCCGC(4/6) FspI 1241 TGCGCA TGC^GCA HhaI 1242 GCGC GCG^C Hin1I 1144 GACGTC G[GA]^CG[CT]C HinP1I 1242 GCGC G^CGC PaeR7I 797 CTCGAG C^TCGAG PflFI 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1485 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 9 CACGTG CAC^GTG PmlI 9 CACGTG CAC^GTG PpuMI 1219 GGGACCC [GA]G^G[AT]CC[CT] PvuII 40 CAGCTG CAG^CTG SacI 15 GAGCTC GAGCT^C Tth111I 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1485 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 797 CTCGAG C^TCGAG XmnI 784 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 8 ACACGT A^C[GA][CT]GT 955 ACATGT ApaI 492 GGGCCC GGGCC^C 806 GGGCCC AvaI 87 CTCGGG C^[CT]CG[GA]G 797 CTCGAG BfaI 739 CTAG C^TAG 1669 CTAG BsmFI 511 GGGAC GGGAC(10/14) 1219 GGGAC BsoBI 87 CTCGGG C^[CT]CG[GA]G 797 CTCGAG DraIII 1067 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1612 CACAATGTG DsaI 1307 CCATGG C^C[GA][CT]GG 1431 CCATGG MaeI 739 CTAG C^TAG 1669 CTAG MaeII 10 ACGT A^CGT 1145 ACGT MslI 916 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1109 CACCTCAGTG MspA1I 40 CAGCTG C[AC]G^C[GT]G 67 CAGCGG NcoI 1307 CCATGG C^CATGG 1431 CCATGG NspI 645 ACATGC [GA]CATG^[CT] 955 ACATGT PstI 64 CTGCAG CTGCA^G 358 CTGCAG SmlI 335 CTCAAG C^T[CT][GA]AG 797 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI BanII BbvI BmyI BsaBI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MamI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII