(Circular) (Six-base) MAPSORT of: pFN21AE1163.seq Check: 4096 from: 1 to: 6445 pFN21AE1163 6445 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3153 4255 278 Size: 53 83 186 936 1617 1102 2468 Fragments arranged by size: 2468 1617 1102 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2589 2884 4508 5892 6160 1632 Size: 555 402 295 1624 1384 268 1917 Fragments arranged by size: 1917 1624 1384 555 402 295 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 2013 2960 5344 6321 1203 Size: 810 947 2384 977 1327 Fragments arranged by size: 2384 1327 977 947 810 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 3118 1166 Size: 1952 4493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2388 1184 Size: 1204 5241 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 2684 Size: 32 6413 AlwNI CAG_nnn'CTG Cuts at: 2213 2231 2519 2567 3496 4188 5760 2213 Size: 18 288 48 929 692 1572 2898 Fragments arranged by size: 2898 1572 929 692 288 48 18 ApaI G_GGCC'C Cuts at: 2501 2815 2501 Size: 314 6131 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 2792 2825 3739 3754 3980 4132 6241 1493 Size: 1299 33 914 15 226 152 2109 1697 Fragments arranged by size: 2109 1697 1299 914 226 152 33 15 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2802 3770 1870 Size: 73 149 710 968 4545 Fragments arranged by size: 4545 968 710 149 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3139 3172 3139 Size: 33 6412 BamHI G'GATC_C Cuts at: 3663 3775 4142 3663 Size: 112 367 5966 Fragments arranged by size: 5966 367 112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3766 5221 618 Size: 325 217 627 1979 1455 1842 Fragments arranged by size: 1979 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2501 2815 3764 1391 Size: 633 477 314 949 4072 Fragments arranged by size: 4072 949 633 477 314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3386 928 Size: 971 1487 3987 Fragments arranged by size: 3987 1487 971 BbvCI CC'TCA_GC Cuts at: 3519 3519 Size: 6445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2968 4337 5547 2968 Size: 1369 1210 3866 Fragments arranged by size: 3866 1369 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 3862 3862 Size: 6445 Bme1580I G_kGCm'C Cuts at: 1790 2100 2501 2815 4506 5662 1790 Size: 310 401 314 1691 1156 2573 Fragments arranged by size: 2573 1691 1156 401 314 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2150 4176 5134 Size: 416 53 83 399 24 733 2026 958 Cuts at: 5134 442 Size: 1753 Fragments arranged by size: 2026 1753 958 733 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2693 5105 1239 Size: 1454 2412 2579 Fragments arranged by size: 2579 2412 1454 Bpu10I CC'TnA_GC Cuts at: 2187 2379 3519 2187 Size: 192 1140 5113 Fragments arranged by size: 5113 1140 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2325 4570 5435 5733 2325 Size: 2245 865 298 3037 Fragments arranged by size: 3037 2245 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2411 5108 882 Size: 617 912 2697 2219 Fragments arranged by size: 2697 2219 912 617 BsaAI yAC'GTr Cuts at: 493 1204 2016 4236 493 Size: 711 812 2220 2702 Fragments arranged by size: 2702 2220 812 711 BsaBI GATnn'nnATC Cuts at: 2424 2667 2874 6439 2424 Size: 243 207 3565 2430 Fragments arranged by size: 3565 2430 243 207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3150 4252 4634 5269 Size: 53 83 186 936 1617 1102 382 635 Cuts at: 5269 275 Size: 1451 Fragments arranged by size: 1617 1451 1102 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3316 3346 1178 Size: 30 165 30 1913 30 4277 Fragments arranged by size: 4277 1913 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2864 1186 Size: 1678 4767 BseYI C'CCAG_C Cuts at: 2192 5648 6144 2192 Size: 3456 496 2493 Fragments arranged by size: 3456 2493 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 2562 2879 3117 1163 Size: 573 415 411 317 238 4491 Fragments arranged by size: 4491 573 415 411 317 238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 4506 4591 5662 2024 Size: 2482 85 1071 2807 Fragments arranged by size: 2807 2482 1071 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2100 2501 2815 3764 4506 4591 Size: 399 234 76 401 314 949 742 85 Cuts at: 4591 5662 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 949 742 401 399 314 234 85 76 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 4334 6064 4334 Size: 1730 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 3800 844 Size: 2143 813 3489 Fragments arranged by size: 3489 2143 813 BsrBI CCG'CTC Cuts at: 1394 1823 4332 1394 Size: 429 2509 3507 Fragments arranged by size: 3507 2509 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2279 4940 5114 65 Size: 1183 1031 2661 174 1396 Fragments arranged by size: 2661 1396 1183 1031 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5089 5276 1700 Size: 110 3279 187 2869 Fragments arranged by size: 3279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6445 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstXI CCAn_nnnn'nTGG Cuts at: 3120 3319 3659 3120 Size: 199 340 5906 Fragments arranged by size: 5906 340 199 BstYI r'GATC_y Cuts at: 1972 2104 2227 2739 3123 3663 3775 4142 4527 Size: 132 123 512 384 540 112 367 385 Cuts at: 4527 4544 5996 6082 6094 6440 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 540 512 385 384 367 346 132 123 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3312 3436 513 Size: 552 2247 124 3522 Fragments arranged by size: 3522 2247 552 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5706 Fragments arranged by size: 5706 683 56 BtsI GCAGTG_nn' Cuts at: 3448 3961 4755 4775 3448 Size: 513 794 20 5118 Fragments arranged by size: 5118 794 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 3077 3622 3077 Size: 545 5900 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5452 809 Size: 507 4136 1802 Fragments arranged by size: 4136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3892 4781 6410 Size: 54 1425 50 275 18 2062 889 1629 Cuts at: 6410 8 Size: 43 Fragments arranged by size: 2062 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2608 3113 3293 4375 1353 Size: 1255 505 180 1082 3423 Fragments arranged by size: 3423 1255 1082 505 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 5032 5406 5552 387 Size: 1064 138 506 2937 374 146 1280 Fragments arranged by size: 2937 1280 1064 506 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2022 Size: 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2589 2693 2884 4508 5105 5892 Size: 393 555 402 104 191 1624 597 787 Cuts at: 5892 6160 1239 Size: 268 1524 Fragments arranged by size: 1624 1524 787 597 555 402 393 268 191 104 EcoO109I rG'GnC_Cy Cuts at: 2497 2498 3025 3225 2497 Size: 1 527 200 5717 Fragments arranged by size: 5717 527 200 1 EcoRI G'AATT_C Cuts at: 2825 3754 4132 2825 Size: 929 378 5138 Fragments arranged by size: 5138 929 378 EcoRV GAT'ATC Cuts at: 3136 3136 Size: 6445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 6445 FspI TGC'GCA Cuts at: 3248 4951 3248 Size: 1703 4742 HaeII r_GCGC'y Cuts at: 1678 5592 1678 Size: 3914 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 2895 2927 3316 Size: 32 135 32 710 32 581 32 389 Cuts at: 3316 3348 5086 5118 5160 5192 1373 Size: 32 1738 32 42 32 2626 Fragments arranged by size: 2626 1738 710 581 389 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2159 3061 5559 5743 1305 Size: 684 170 902 2498 184 2007 Fragments arranged by size: 2498 2007 902 684 184 170 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4623 Fragments arranged by size: 4623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2925 3118 4403 4762 4921 518 Size: 648 1759 193 1285 359 159 2042 Fragments arranged by size: 2042 1759 1285 648 359 193 159 MspA1I CmG'CkG Cuts at: 1606 2047 2074 3861 4538 5686 5931 1606 Size: 441 27 1787 677 1148 245 2120 Fragments arranged by size: 2120 1787 1148 677 441 245 27 NcoI C'CATG_G Cuts at: 513 1065 3312 3436 513 Size: 552 2247 124 3522 Fragments arranged by size: 3522 2247 552 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 2654 2964 3803 5348 1132 Size: 97 1425 310 839 1545 2229 Fragments arranged by size: 2229 1545 1425 839 310 97 PasI CC'CwG_GG Cuts at: 1348 2501 3195 1348 Size: 1153 694 4598 Fragments arranged by size: 4598 1153 694 PciI A'CATG_T Cuts at: 2960 5344 2960 Size: 2384 4061 PflMI CCAn_nnn'nTGG Cuts at: 1108 3496 1108 Size: 2388 4057 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PmlI CAC'GTG Cuts at: 2016 2016 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3225 3225 Size: 6445 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 2497 2811 2497 Size: 314 6131 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3507 3539 3507 Size: 32 6413 PstI C_TGCA'G Cuts at: 830 2073 2367 3797 830 Size: 1243 294 1430 3478 Fragments arranged by size: 3478 1430 1243 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2047 2047 Size: 6445 SacI G_AGCT'C Cuts at: 2024 2024 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SanDI GG'GwC_CC Cuts at: 3225 3225 Size: 6445 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2069 2223 2363 2559 3269 3793 4928 Size: 219 1024 154 140 196 710 524 1135 Cuts at: 4928 5609 5800 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1024 710 681 524 219 196 191 154 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3772 1872 Size: 1900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2340 2802 4549 5450 5712 820 Size: 197 926 397 462 1747 901 262 1553 Fragments arranged by size: 1747 1553 926 901 462 397 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6445 StyI C'CwwG_G Cuts at: 513 1065 2769 2844 3312 3436 3478 3643 3884 Size: 552 1704 75 468 124 42 165 241 Cuts at: 3884 513 Size: 3074 Fragments arranged by size: 3074 1704 552 468 241 165 124 75 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 2465 3117 3364 4472 4489 4642 Size: 1255 605 280 652 247 1108 17 153 Cuts at: 4642 4827 5272 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1108 652 605 445 280 247 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4691 6403 96 Size: 274 80 33 51 1443 2714 1712 138 Fragments arranged by size: 2714 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3115 3147 6405 6437 3115 Size: 32 3258 32 3123 Fragments arranged by size: 3258 3123 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2883 1316 Size: 1567 4878 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 2802 1943 Size: 859 5586 XmaI C'CCGG_G Cuts at: 1870 3770 1870 Size: 1900 4545 XmnI GAAnn'nnTTC Cuts at: 2793 4574 2793 Size: 1781 4664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3151 4253 276 Size: 53 83 186 936 1617 1102 2468 Fragments arranged by size: 2468 1617 1102 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI