(Linear) (Six-base) MAPSORT of: KIEE1163.seq Check: 3488 from: 1 to: 1740 KIEE1163.orf With 182 enzymes: SgfI * July 3, 2012 16:48 .. AatII G_ACGT'C Cuts at: 0 1148 1740 Size: 1148 592 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 584 879 1740 Size: 182 402 295 861 Fragments arranged by size: 861 402 295 182 AflIII A'CryG_T Cuts at: 0 8 955 1740 Size: 8 947 785 Fragments arranged by size: 947 785 8 AleI CACnn'nnGTG Cuts at: 0 1113 1740 Size: 1113 627 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 383 1740 Size: 383 1357 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 679 711 1740 Size: 679 32 1029 Fragments arranged by size: 1029 679 32 AlwNI CAG_nnn'CTG Cuts at: 0 208 226 514 562 1491 1740 Size: 208 18 288 48 929 249 Fragments arranged by size: 929 288 249 208 48 18 ApaI G_GGCC'C Cuts at: 0 496 810 1740 Size: 496 314 930 Fragments arranged by size: 930 496 314 ApoI r'AATT_y Cuts at: 0 787 820 1734 1740 Size: 787 33 914 6 Fragments arranged by size: 914 787 33 6 AvaI C'yCGr_G Cuts at: 0 87 797 1740 Size: 87 710 943 Fragments arranged by size: 943 710 87 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1134 1167 1740 Size: 1134 33 573 Fragments arranged by size: 1134 573 33 BamHI G'GATC_C Cuts at: 0 1658 1740 Size: 1658 82 BanII G_rGCy'C Cuts at: 0 19 496 810 1740 Size: 19 477 314 930 Fragments arranged by size: 930 477 314 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 1381 1740 Size: 1381 359 BbvCI CC'TCA_GC Cuts at: 0 1514 1740 Size: 1514 226 BciVI GTATCCnnnnn_n' Cuts at: 0 963 1740 Size: 963 777 Bme1580I G_kGCm'C Cuts at: 0 95 496 810 1740 Size: 95 401 314 930 Fragments arranged by size: 930 401 314 95 BmrI ACTGGGnnnn_n' Cuts at: 0 145 1740 Size: 145 1595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1740 Size: 688 1052 Bpu10I CC'TnA_GC Cuts at: 0 182 374 1514 1740 Size: 182 192 1140 226 Fragments arranged by size: 1140 226 192 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 1740 Size: 320 1420 BsaI GGTCTCn'nnnn_ Cuts at: 0 406 1740 Size: 406 1334 BsaAI yAC'GTr Cuts at: 0 11 1740 Size: 11 1729 BsaBI GATnn'nnATC Cuts at: 0 419 662 869 1740 Size: 419 243 207 871 Fragments arranged by size: 871 419 243 207 BsaHI Gr'CG_yC Cuts at: 0 1145 1740 Size: 1145 595 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1311 1341 1740 Size: 1311 30 399 Fragments arranged by size: 1311 399 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 859 1740 Size: 859 881 BseYI C'CCAG_C Cuts at: 0 187 1740 Size: 187 1553 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 557 874 1112 1740 Size: 146 411 317 238 628 Fragments arranged by size: 628 411 317 238 146 BsiHKAI G_wGCw'C Cuts at: 0 19 1740 Size: 19 1721 Bsp1286I G_dGCh'C Cuts at: 0 19 95 496 810 1740 Size: 19 76 401 314 930 Fragments arranged by size: 930 401 314 76 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 982 1740 Size: 982 758 BsrDI GCAATG_nn' Cuts at: 0 274 1740 Size: 274 1466 BstXI CCAn_nnnn'nTGG Cuts at: 0 1115 1314 1654 1740 Size: 1115 199 340 86 Fragments arranged by size: 1115 340 199 86 BstYI r'GATC_y Cuts at: 0 99 222 734 1118 1658 1740 Size: 99 123 512 384 540 82 Fragments arranged by size: 540 512 384 123 99 82 BtgI C'CryG_G Cuts at: 0 1307 1431 1740 Size: 1307 124 309 Fragments arranged by size: 1307 309 124 BtsI GCAGTG_nn' Cuts at: 0 1443 1740 Size: 1443 297 DraIII CAC_nnn'GTG Cuts at: 0 1072 1617 1740 Size: 1072 545 123 Fragments arranged by size: 1072 545 123 EarI CTCTTCn'nnn_ Cuts at: 0 603 1108 1288 1740 Size: 603 505 180 452 Fragments arranged by size: 603 505 452 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 90 1740 Size: 90 1650 EcoICRI GAG'CTC Cuts at: 0 17 1740 Size: 17 1723 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 584 688 879 1740 Size: 182 402 104 191 861 Fragments arranged by size: 861 402 191 182 104 EcoO109I rG'GnC_Cy Cuts at: 0 492 493 1020 1220 1740 Size: 492 1 527 200 520 Fragments arranged by size: 527 520 492 200 1 EcoRI G'AATT_C Cuts at: 0 820 1740 Size: 820 920 EcoRV GAT'ATC Cuts at: 0 1131 1740 Size: 1131 609 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 909 1740 Size: 909 831 FspI TGC'GCA Cuts at: 0 1243 1740 Size: 1243 497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 277 309 890 922 1311 1343 1740 Size: 277 32 581 32 389 32 397 Fragments arranged by size: 581 397 389 277 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1056 1740 Size: 154 902 684 Fragments arranged by size: 902 684 154 MslI CAynn'nnrTG Cuts at: 0 920 1113 1740 Size: 920 193 627 Fragments arranged by size: 920 627 193 MspA1I CmG'CkG Cuts at: 0 42 69 1740 Size: 42 27 1671 Fragments arranged by size: 1671 42 27 NcoI C'CATG_G Cuts at: 0 1307 1431 1740 Size: 1307 124 309 Fragments arranged by size: 1307 309 124 NspI r_CATG'y Cuts at: 0 649 959 1740 Size: 649 310 781 Fragments arranged by size: 781 649 310 PasI CC'CwG_GG Cuts at: 0 496 1190 1740 Size: 496 694 550 Fragments arranged by size: 694 550 496 PciI A'CATG_T Cuts at: 0 955 1740 Size: 955 785 PflMI CCAn_nnn'nTGG Cuts at: 0 1491 1740 Size: 1491 249 PmlI CAC'GTG Cuts at: 0 11 1740 Size: 11 1729 PpuMI rG'GwC_Cy Cuts at: 0 1220 1740 Size: 1220 520 PspOMI G'GGCC_C Cuts at: 0 492 806 1740 Size: 492 314 934 Fragments arranged by size: 934 492 314 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1502 1534 1740 Size: 1502 32 206 Fragments arranged by size: 1502 206 32 PstI C_TGCA'G Cuts at: 0 68 362 1740 Size: 68 294 1378 Fragments arranged by size: 1378 294 68 PvuII CAG'CTG Cuts at: 0 42 1740 Size: 42 1698 SacI G_AGCT'C Cuts at: 0 19 1740 Size: 19 1721 SanDI GG'GwC_CC Cuts at: 0 1220 1740 Size: 1220 520 SfcI C'TryA_G Cuts at: 0 64 218 358 554 1264 1740 Size: 64 154 140 196 710 476 Fragments arranged by size: 710 476 196 154 140 64 SmlI C'TyrA_G Cuts at: 0 335 797 1740 Size: 335 462 943 Fragments arranged by size: 943 462 335 StyI C'CwwG_G Cuts at: 0 764 839 1307 1431 1473 1638 1740 Size: 764 75 468 124 42 165 102 Fragments arranged by size: 764 468 165 124 102 75 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 180 460 1112 1359 1740 Size: 180 280 652 247 381 Fragments arranged by size: 652 381 280 247 180 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1110 1142 1740 Size: 1110 32 598 Fragments arranged by size: 1110 598 32 Tth111I GACn'n_nGTC Cuts at: 0 878 1740 Size: 878 862 XhoI C'TCGA_G Cuts at: 0 797 1740 Size: 797 943 XmnI GAAnn'nnTTC Cuts at: 0 788 1740 Size: 788 952 ZraI GAC'GTC Cuts at: 0 1146 1740 Size: 1146 594 Enzymes that do cut: AatII AcuI AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanII BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstXI BstYI BtgI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I MmeI MslI MspA1I NcoI NspI PasI PciI PflMI PmlI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SfcI SmlI StyI TaqII TstI Tth111I XhoI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI ApaLI AscI AseI AsiSI AvrII BanI BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoNI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XmaI