################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 721 GTAGAC GT^[AC][GT]AC AcsI 652 AAATTC [GA]^AATT[CT] ApoI 652 AAATTC [GA]^AATT[CT] BalI 410 TGGCCA TGG^CCA BanI 117 GGTGCC G^G[CT][GA]CC BseRI 372 CTCCTC GAGGAG(10/8) BsmAI 561 GTCTC GTCTC(1/5) Bsp1407I 254 TGTACA T^GTACA BsrGI 254 TGTACA T^GTACA BstXI 263 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 740 AGATCC [GA]^GATC[CT] CfoI 933 GCGC GCG^C DraIII 731 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 410 TGGCCA [CT]^GGCC[GA] FauI 466 GCGGG CCCGC(4/6) HhaI 933 GCGC GCG^C HinP1I 933 GCGC G^CGC HindIII 1049 AAGCTT A^AGCTT MflI 740 AGATCC [GA]^GATC[CT] MluNI 410 TGGCCA TGG^CCA MscI 410 TGGCCA TGG^CCA NcoI 503 CCATGG C^CATGG NspI 251 GCATGT [GA]CATG^[CT] SexAI 278 ACCTGGT A^CC[AT]GGT SfaNI 877 GCATC GCATC(5/9) SfcI 597 CTACAG C^T[GA][CT]AG SspBI 254 TGTACA T^GTACA TfiI 608 GATTC G^A[AT]TC Tsp45I 673 GTCAC ^GT[GC]AC XhoII 740 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AocI 62 CCTTAGG CC^T[ACGT]AGG 565 CCTGAGG AvaII 941 GGTCC G^G[AT]CC 963 GGACC BpmI 220 CTCCAG CTGGAG(16/14) 144 CTGGAG BspMI 929 ACCTGC ACCTGC(4/8) 851 GCAGGT Bsu36I 62 CCTTAGG CC^T[ACGT]AGG 565 CCTGAGG DsaI 377 CCACGG C^C[GA][CT]GG 503 CCATGG Eco81I 62 CCTTAGG CC^T[ACGT]AGG 565 CCTGAGG EcoT14I 174 CCAAGG C^C[AT][AT]GG 503 CCATGG MaeIII 533 GTAAC ^GT[ACGT]AC 673 GTCAC PflMI 174 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCAGACCCTGG PleI 41 GACTC GAGTC(4/5) 447 GACTC SacI 190 GAGCTC GAGCT^C 837 GAGCTC StyI 174 CCAAGG C^C[AT][AT]GG 503 CCATGG Van91I 174 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCAGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI BanII BbvI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI SapI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI