(Circular) (Six-base) MAPSORT of: pFN21AE5583.seq Check: 8876 from: 1 to: 5791 pFN21AE5583 5791 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3134 1937 Size: 790 407 4594 Fragments arranged by size: 4594 790 407 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2366 3854 5238 5506 1632 Size: 406 328 1488 1384 268 1917 Fragments arranged by size: 1917 1488 1384 406 328 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 2387 2996 1166 Size: 1221 609 3961 Fragments arranged by size: 3961 1221 609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2233 2401 2981 3534 5106 2233 Size: 168 580 553 1572 2918 Fragments arranged by size: 2918 1572 580 553 168 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 2657 3100 3326 3478 5587 1493 Size: 1164 443 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 443 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 3116 1870 Size: 73 1173 4545 Fragments arranged by size: 4545 1173 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3112 4567 618 Size: 325 217 627 335 990 1455 1842 Fragments arranged by size: 1842 1455 990 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2199 2600 2846 3110 1391 Size: 705 103 401 246 264 4072 Fragments arranged by size: 4072 705 401 264 246 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2394 3852 5008 1790 Size: 604 1458 1156 2573 Fragments arranged by size: 2573 1458 1156 604 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2079 2999 3522 Size: 416 53 83 399 24 662 920 523 Cuts at: 3522 4480 442 Size: 958 1753 Fragments arranged by size: 1753 958 920 662 523 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2170 2210 4451 1239 Size: 931 40 2241 2579 Fragments arranged by size: 2579 2241 931 40 Bpu10I CC'TnA_GC Cuts at: 2174 2174 Size: 5791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4454 882 Size: 617 2955 2219 Fragments arranged by size: 2955 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3598 3980 4615 275 Size: 53 83 186 936 2065 382 635 1451 Fragments arranged by size: 2065 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5566 Fragments arranged by size: 5566 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2368 1186 Size: 1182 4609 BseYI C'CCAG_C Cuts at: 2220 2275 4994 5490 2220 Size: 55 2719 496 2521 Fragments arranged by size: 2719 2521 496 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2628 2770 1163 Size: 573 570 322 142 4184 Fragments arranged by size: 4184 573 570 322 142 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2199 2671 2846 3852 3937 5008 2148 Size: 51 472 175 1006 85 1071 2931 Fragments arranged by size: 2931 1071 1006 472 175 85 51 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2148 2199 2394 2600 2671 2846 Size: 399 306 52 51 195 206 71 175 Cuts at: 2846 3110 3852 3937 5008 1391 Size: 264 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 264 206 195 175 85 71 52 51 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2847 2943 3146 844 Size: 2003 96 203 3489 Fragments arranged by size: 3489 2003 203 96 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4435 4622 1700 Size: 110 2625 187 2869 Fragments arranged by size: 2869 2625 187 110 BsrGI T'GTAC_A Cuts at: 96 2259 96 Size: 2163 3628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2275 2275 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2745 3121 3488 3873 3890 5342 5428 5440 Size: 773 376 367 385 17 1452 86 12 Cuts at: 5440 5786 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 773 385 376 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2068 2571 2068 Size: 503 5288 BtgI C'CryG_G Cuts at: 513 1065 2382 2508 513 Size: 552 1317 126 3796 Fragments arranged by size: 3796 1317 552 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 2485 487 Size: 683 56 896 363 3793 Fragments arranged by size: 3793 896 683 363 56 BtsI GCAGTG_nn' Cuts at: 2892 3307 4101 4121 2892 Size: 415 794 20 4562 Fragments arranged by size: 4562 794 415 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DraIII CAC_nnn'GTG Cuts at: 2741 2741 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2415 3238 4127 Size: 54 1425 50 275 18 585 823 889 Cuts at: 4127 5756 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 823 585 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 2342 2759 2912 3721 1353 Size: 890 99 417 153 809 3423 Fragments arranged by size: 3423 890 809 417 153 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2197 2844 2197 Size: 647 5144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2170 2210 2366 3854 4451 5238 Size: 393 406 132 40 156 1488 597 787 Cuts at: 5238 5506 1239 Size: 268 1524 Fragments arranged by size: 1524 1488 787 597 406 393 268 156 132 40 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 4938 1678 Size: 3260 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373 Size: 32 135 32 2860 32 42 32 2626 Fragments arranged by size: 2860 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 3054 748 Size: 2306 3485 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 10 Size: 54 1425 343 585 3384 Fragments arranged by size: 3384 1425 585 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2310 2330 2387 2996 3749 4108 4267 Size: 648 1144 20 57 609 753 359 159 Cuts at: 4267 518 Size: 2042 Fragments arranged by size: 2042 1144 753 648 609 359 159 57 20 MspA1I CmG'CkG Cuts at: 1606 3207 3884 5032 5277 1606 Size: 1601 677 1148 245 2120 Fragments arranged by size: 2120 1601 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2508 513 Size: 552 1443 3796 Fragments arranged by size: 3796 1443 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2260 3149 4694 1132 Size: 97 1031 889 1545 2229 Fragments arranged by size: 2229 1545 1031 889 97 PasI CC'CwG_GG Cuts at: 1348 2851 1348 Size: 1503 4288 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 2401 1108 Size: 1077 216 4498 Fragments arranged by size: 4498 1077 216 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 SacI G_AGCT'C Cuts at: 2199 2846 2199 Size: 647 5144 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SapI GCTCTTCn'nnn_ Cuts at: 2243 2342 2759 2243 Size: 99 417 5275 Fragments arranged by size: 5275 417 99 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SexAI A'CCwGG_T Cuts at: 2283 2283 Size: 5791 SfcI C'TryA_G Cuts at: 826 1045 2602 3139 4274 4955 5146 826 Size: 219 1557 537 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 681 537 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3895 4796 5058 820 Size: 197 926 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5791 StyI C'CwwG_G Cuts at: 513 1065 2179 2508 3230 513 Size: 552 1114 329 722 3074 Fragments arranged by size: 3074 1114 722 552 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 2719 3818 3835 3988 4173 4618 Size: 1255 764 375 1099 17 153 185 445 Cuts at: 4618 325 Size: 1498 Fragments arranged by size: 1498 1255 1099 764 445 375 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 2334 3024 Size: 274 80 33 51 1443 282 75 690 Cuts at: 3024 4037 5749 96 Size: 1013 1712 138 Fragments arranged by size: 1712 1443 1013 690 282 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI