(Linear) (Six-base) MAPSORT of: KIEE5489.seq Check: 4150 from: 1 to: 1029 KIEE5489.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AccI GT'mk_AC Cuts at: 0 614 1029 Size: 614 415 AleI CACnn'nnGTG Cuts at: 0 829 1029 Size: 829 200 ApoI r'AATT_y Cuts at: 0 124 1011 1029 Size: 124 887 18 Fragments arranged by size: 887 124 18 BfrBI ATG'CAT Cuts at: 0 854 1029 Size: 854 175 BmrI ACTGGGnnnn_n' Cuts at: 0 421 794 1029 Size: 421 373 235 Fragments arranged by size: 421 373 235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1017 1029 Size: 1017 12 Bpu10I CC'TnA_GC Cuts at: 0 715 1029 Size: 715 314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 98 1029 Size: 98 931 BsaI GGTCTCn'nnnn_ Cuts at: 0 444 553 1029 Size: 444 109 476 Fragments arranged by size: 476 444 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 8 38 1029 Size: 8 30 991 Fragments arranged by size: 991 30 8 BseYI C'CCAG_C Cuts at: 0 135 376 1029 Size: 135 241 653 Fragments arranged by size: 653 241 135 BspHI T'CATG_A Cuts at: 0 497 1029 Size: 497 532 BsrGI T'GTAC_A Cuts at: 0 675 1029 Size: 675 354 BstEII G'GTnAC_C Cuts at: 0 214 1029 Size: 214 815 Bsu36I CC'TnA_GG Cuts at: 0 329 1029 Size: 329 700 BtgI C'CryG_G Cuts at: 0 27 443 1029 Size: 27 416 586 Fragments arranged by size: 586 416 27 EaeI y'GGCC_r Cuts at: 0 464 1029 Size: 464 565 EarI CTCTTCn'nnn_ Cuts at: 0 187 844 1029 Size: 187 657 185 Fragments arranged by size: 657 187 185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1017 1029 Size: 1017 12 EcoRI G'AATT_C Cuts at: 0 124 1011 1029 Size: 124 887 18 Fragments arranged by size: 887 124 18 EcoRV GAT'ATC Cuts at: 0 980 1029 Size: 980 49 HindIII A'AGCT_T Cuts at: 0 649 1029 Size: 649 380 MscI TGG'CCA Cuts at: 0 466 1029 Size: 466 563 MslI CAynn'nnrTG Cuts at: 0 826 829 1029 Size: 826 3 200 Fragments arranged by size: 826 200 3 NcoI C'CATG_G Cuts at: 0 27 443 1029 Size: 27 416 586 Fragments arranged by size: 586 416 27 NsiI A_TGCA'T Cuts at: 0 856 1029 Size: 856 173 SfcI C'TryA_G Cuts at: 0 56 78 744 890 1029 Size: 56 22 666 146 139 Fragments arranged by size: 666 146 139 56 22 SmlI C'TyrA_G Cuts at: 0 113 1029 Size: 113 916 StyI C'CwwG_G Cuts at: 0 27 279 443 1029 Size: 27 252 164 586 Fragments arranged by size: 586 252 164 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 368 403 1029 Size: 368 35 626 Fragments arranged by size: 626 368 35 TatI w'GTAC_w Cuts at: 0 675 1029 Size: 675 354 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 8 40 1029 Size: 8 32 989 Fragments arranged by size: 989 32 8 XbaI T'CTAG_A Cuts at: 0 1015 1029 Size: 1015 14 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 781 1029 Size: 781 248 Enzymes that do cut: AccI AleI ApoI BfrBI BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BspHI BsrGI BstEII Bsu36I BtgI EaeI EarI Eco57MI EcoRI EcoRV HindIII MscI MslI NcoI NsiI SfcI SmlI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI XmnI ZraI