Restriction map for
pFN21AE5980
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 413 GACGTC GACGT^C
AccIII 554 TCCGGA T^CCGGA
AciI 363 CCGC CCGC(-3/-1)
AcsI 31 GAATTT [GA]^AATT[CT]
AcyI 413 GACGTC G[GA]^CG[CT]C
AfaI 216 GTAC GT^AC
AluI 299 AGCT AG^CT
ApaI 508 GGGCCC GGGCC^C
ApoI 31 GAATTT [GA]^AATT[CT]
Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 59 CTCGGG C^[CT]CG[GA]G
AvaII 66 GGTCC G^G[AT]CC
BanII 508 GGGCCC G[GA]GC[CT]^C
BcgI 412 CGACGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 425 GTATCC GTATCC(6/5)
BmyI 508 GGGCCC G[AGT]GC[ACT]^C
BsaHI 413 GACGTC G[GA]^CG[CT]C
BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 554 TCCGGA [AT]^CCGG[AT]
BseAI 554 TCCGGA T^CCGGA
BseRI 583 GAGGAG GAGGAG(10/8)
BsmAI 416 GTCTC GTCTC(1/5)
BsmBI 415 CGTCTC CGTCTC(1/5)
BsoBI 59 CTCGGG C^[CT]CG[GA]G
Bsp1286I 508 GGGCCC G[AGT]GC[ACT]^C
BspEI 554 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BspMI 721 ACCTGC ACCTGC(4/8)
BsrI 43 ACTGG ACTGG(1/-1)
BstEII 909 GGTCACC G^GT[ACGT]ACC
BstPI 909 GGTCACC G^GT[ACGT]ACC
Cac8I 781 GCTCGC GC[ACGT]^[ACGT]GC
DdeI 690 CTCAG C^T[ACGT]AG
EcoO65I 909 GGTCACC G^GT[ACGT]ACC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
EcoT22I 288 ATGCAT ATGCA^T
HapII 555 CCGG C^CGG
Hin1I 413 GACGTC G[GA]^CG[CT]C
HpaII 555 CCGG C^CGG
MaeII 414 ACGT A^CGT
MroI 554 TCCGGA T^CCGGA
MspI 555 CCGG C^CGG
MwoI 190 GCTAATCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 508 GGGCCC GG[ACGT]^[ACGT]CC
NsiI 288 ATGCAT ATGCA^T
RcaI 404 TCATGA T^CATGA
RsaI 216 GTAC GT^AC
SapI 300 GCTCTTC GCTCTTC(1/4)
SfaNI 287 GATGC GCATC(5/9)
SfcI 600 CTGTAG C^T[GA][CT]AG
SmlI 673 CTCAAG C^T[CT][GA]AG
SpeI 72 ACTAGT A^CTAGT
SspI 837 AATATT AAT^ATT
StyI 20 CCAAGG C^C[AT][AT]GG
TfiI 408 GAATC G^A[AT]TC
XcmI 896 CCATGAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbvI 892 GCAGC GCAGC(8/12)
370 GCTGC
BsrDI 291 CATTGC GCAATG(2/0)
633 CATTGC
DpnII 597 GATC ^GATC
615 GATC
Fnu4HI 370 GCTGC GC^[ACGT]GC
892 GCAGC
ItaI 370 GCTGC GC^[ACGT]GC
892 GCAGC
MboI 597 GATC ^GATC
615 GATC
MseI 643 TTAA T^TAA
851 TTAA
NdeII 597 GATC ^GATC
615 GATC
PleI 57 GACTC GAGTC(4/5)
793 GACTC
Sau3AI 597 GATC ^GATC
615 GATC
TaqI 411 TCGA T^CGA
492 TCGA
Tru9I 643 TTAA T^TAA
851 TTAA
TseI 370 GCTGC G^C[AT]GC
892 GCAGC
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
TthHB8I 411 TCGA T^CGA
492 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BfaI BsiYI BslI BssKI BstF5I
BstNI Cfr13I EarI EcoRII FokI
HaeIII HinfI HphI Ksp632I MaeI
MaeIII MboII MnlI MslI MvaI
NlaIII Sau96I ScrFI Tsp45I Tsp509I