(Circular) (Six-base) MAPSORT of: pFN21AE2133.seq Check: 6074 from: 1 to: 7684 pFN21AE2133 7684 bp With 182 enzymes: SgfI * March 7, 2011 13:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5494 278 Size: 53 83 186 936 3958 2468 Fragments arranged by size: 3958 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5027 1937 Size: 3090 4594 Acc65I G'GTAC_C Cuts at: 5005 5005 Size: 7684 AclI AA'CG_TT Cuts at: 1563 5811 6184 1563 Size: 4248 373 3063 Fragments arranged by size: 4248 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4311 5747 7131 7399 1632 Size: 2679 1436 1384 268 1917 Fragments arranged by size: 2679 1917 1436 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7487 AflIII A'CryG_T Cuts at: 1203 6583 7560 1203 Size: 5380 977 1327 Fragments arranged by size: 5380 1327 977 AgeI A'CCGG_T Cuts at: 6515 6515 Size: 7684 AhdI GACnn_n'nnGTC Cuts at: 6413 6413 Size: 7684 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7684 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3388 3420 3388 Size: 32 7652 AlwNI CAG_nnn'CTG Cuts at: 5427 6999 5427 Size: 1572 6112 ApaLI G'TGCA_C Cuts at: 4045 5741 6897 4045 Size: 1696 1156 4832 Fragments arranged by size: 4832 1696 1156 ApoI r'AATT_y Cuts at: 1493 3829 4130 4204 4276 4403 4412 4993 5219 Size: 2336 301 74 72 127 9 581 226 Cuts at: 5219 5371 7480 1493 Size: 152 2109 1697 Fragments arranged by size: 2336 2109 1697 581 301 226 152 127 74 72 9 AseI AT'TA_AT Cuts at: 160 1032 6238 160 Size: 872 5206 1606 Fragments arranged by size: 5206 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7684 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2879 4503 5009 1870 Size: 73 249 687 1624 506 4545 Fragments arranged by size: 4545 1624 687 506 249 73 BamHI G'GATC_C Cuts at: 5014 5381 5014 Size: 367 7317 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5005 6460 618 Size: 325 217 627 3218 1455 1842 Fragments arranged by size: 3218 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2219 2853 5003 1391 Size: 828 634 2150 4072 Fragments arranged by size: 4072 2150 828 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2193 2518 928 Size: 971 294 325 6094 Fragments arranged by size: 6094 971 325 294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3321 3355 5875 5909 1903 Size: 34 50 34 1300 34 2520 34 3678 Fragments arranged by size: 3678 2520 1300 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2975 3197 3365 5576 6786 2975 Size: 222 168 2211 1210 3873 Fragments arranged by size: 3873 2211 1210 222 168 BclI T'GATC_A Cuts at: 1548 2100 1548 Size: 552 7132 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6295 136 Size: 107 122 71 631 605 337 4286 1525 Fragments arranged by size: 4286 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2089 2746 4363 7679 2089 Size: 657 1617 3316 2094 Fragments arranged by size: 3316 2094 1617 657 BlpI GC'TnA_GC Cuts at: 5101 5101 Size: 7684 Bme1580I G_kGCm'C Cuts at: 1790 4049 5745 6901 1790 Size: 2259 1696 1156 2573 Fragments arranged by size: 2573 2259 1696 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7684 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2723 3006 5415 Size: 416 53 83 399 24 1306 283 2409 Cuts at: 5415 6373 442 Size: 958 1753 Fragments arranged by size: 2409 1753 1306 958 416 399 283 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7684 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3519 3992 3519 Size: 473 7211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2439 3582 6344 1239 Size: 1200 1143 2762 2579 Fragments arranged by size: 2762 2579 1200 1143 Bpu10I CC'TnA_GC Cuts at: 2218 4968 2218 Size: 2750 4934 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3306 3974 4777 5809 6674 6972 3306 Size: 668 803 1032 865 298 4018 Fragments arranged by size: 4018 1032 865 803 668 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2200 4428 6347 882 Size: 617 701 2228 1919 2219 Fragments arranged by size: 2228 2219 1919 701 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 5475 493 Size: 711 1946 2325 2702 Fragments arranged by size: 2702 2325 1946 711 BsaBI GATnn'nnATC Cuts at: 2751 7678 2751 Size: 4927 2757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2544 3353 5491 5873 Size: 53 83 186 936 1011 809 2138 382 Cuts at: 5873 6508 275 Size: 635 1451 Fragments arranged by size: 2138 1451 1011 936 809 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2978 4681 6117 6515 6789 6936 7347 2978 Size: 1703 1436 398 274 147 411 3315 Fragments arranged by size: 3315 1703 1436 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7459 Fragments arranged by size: 7459 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 2751 2897 3650 3745 4349 1186 Size: 1202 363 146 753 95 604 4521 Fragments arranged by size: 4521 1202 753 604 363 146 95 BseYI C'CCAG_C Cuts at: 4002 4006 4036 4779 6887 7383 4002 Size: 4 30 743 2108 496 4303 Fragments arranged by size: 4303 2108 743 496 30 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3409 1163 Size: 573 1673 5438 Fragments arranged by size: 5438 1673 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2583 5134 5895 6923 1540 Size: 275 186 582 2551 761 1028 2301 Fragments arranged by size: 2551 2301 1028 761 582 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 3330 4049 5745 5830 6901 2219 Size: 1111 719 1696 85 1071 3002 Fragments arranged by size: 3002 1696 1111 1071 719 85 BsmI GAATG_Cn' Cuts at: 2729 4677 5195 5288 2729 Size: 1948 518 93 5125 Fragments arranged by size: 5125 1948 518 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3572 3572 Size: 7684 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2853 3330 4049 5003 5745 5830 Size: 399 429 634 477 719 954 742 85 Cuts at: 5830 6901 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 954 742 719 634 477 429 399 85 BspEI T'CCGG_A Cuts at: 7347 7347 Size: 7684 BspHI T'CATG_A Cuts at: 2668 4512 5573 7303 2668 Size: 1844 1061 1730 3049 Fragments arranged by size: 3049 1844 1730 1061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4041 5039 844 Size: 3197 998 3489 Fragments arranged by size: 3489 3197 998 BsrBI CCG'CTC Cuts at: 1394 1823 5571 1394 Size: 429 3748 3507 Fragments arranged by size: 3748 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2067 2771 4549 6179 6353 65 Size: 1183 819 704 1778 1630 174 1396 Fragments arranged by size: 1778 1630 1396 1183 819 704 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3749 6328 6515 1700 Size: 110 198 1741 2579 187 2869 Fragments arranged by size: 2869 2579 1741 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7684 BssHII G'CGCG_C Cuts at: 1925 2018 1925 Size: 93 7591 BssSI C'ACGA_G Cuts at: 4016 5744 6756 4016 Size: 1728 1012 4944 Fragments arranged by size: 4944 1728 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2724 794 Size: 1930 5754 BstBI TT'CG_AA Cuts at: 6488 6488 Size: 7684 BstEII G'GTnAC_C Cuts at: 1184 3028 3745 1184 Size: 1844 717 5123 Fragments arranged by size: 5123 1844 717 BstXI CCAn_nnnn'nTGG Cuts at: 4399 4399 Size: 7684 BstYI r'GATC_y Cuts at: 1972 2089 2746 4363 5014 5381 5766 5783 7235 Size: 117 657 1617 651 367 385 17 1452 Cuts at: 7235 7321 7333 7679 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1617 1452 657 651 385 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2166 2385 2839 4465 513 Size: 552 1101 219 454 1626 3732 Fragments arranged by size: 3732 1626 1101 552 454 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4877 487 Size: 683 56 3651 3294 Fragments arranged by size: 3651 3294 683 56 BtsI GCAGTG_nn' Cuts at: 2273 5200 5994 6014 2273 Size: 2927 794 20 3943 Fragments arranged by size: 3943 2927 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7684 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7649 DraI TTT'AAA Cuts at: 3124 3591 4723 4988 3124 Size: 467 1132 265 5820 Fragments arranged by size: 5820 1132 467 265 DraIII CAC_nnn'GTG Cuts at: 4658 4658 Size: 7684 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2549 6691 809 Size: 507 1233 4142 1802 Fragments arranged by size: 4142 1802 1233 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 4440 4802 Size: 54 1425 50 275 18 199 2411 362 Cuts at: 4802 4882 5131 6020 7649 8 Size: 80 249 889 1629 43 Fragments arranged by size: 2411 1629 1425 889 362 275 249 199 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5131 1537 Size: 275 3319 4090 Fragments arranged by size: 4090 3319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3004 3093 3426 3668 4326 5614 1353 Size: 1651 89 333 242 658 1288 3423 Fragments arranged by size: 3423 1651 1288 658 333 242 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6271 6645 6791 387 Size: 1064 138 4682 374 146 1280 Fragments arranged by size: 4682 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2217 2217 Size: 7684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2439 3582 4311 5747 6344 7131 7399 Size: 393 807 1143 729 1436 597 787 268 Cuts at: 7399 1239 Size: 1524 Fragments arranged by size: 1524 1436 1143 807 787 729 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3602 3602 Size: 7684 EcoRI G'AATT_C Cuts at: 4276 4993 5371 4276 Size: 717 378 6589 Fragments arranged by size: 6589 717 378 EcoRV GAT'ATC Cuts at: 4510 4510 Size: 7684 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2532 4385 2532 Size: 1853 5831 FspI TGC'GCA Cuts at: 6190 6190 Size: 7684 HaeII r_GCGC'y Cuts at: 1678 6831 1678 Size: 5153 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2835 2867 3145 3177 3614 Size: 32 135 32 1263 32 278 32 437 Cuts at: 3614 3646 3903 3935 4244 4276 4604 4636 6325 Size: 32 257 32 309 32 328 32 1689 Cuts at: 6325 6357 6399 6431 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1689 1263 437 328 309 278 257 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5028 5274 669 Size: 988 281 3090 246 3079 Fragments arranged by size: 3090 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7684 HpaI GTT'AAC Cuts at: 5274 5274 Size: 7684 KpnI G_GTAC'C Cuts at: 5009 5009 Size: 7684 MfeI C'AATT_G Cuts at: 3645 5283 3645 Size: 1638 6046 MluI A'CGCG_T Cuts at: 7560 7560 Size: 7684 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 6798 6982 1305 Size: 3243 2250 184 2007 Fragments arranged by size: 3243 2250 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5862 Fragments arranged by size: 5862 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4766 5642 6001 6160 518 Size: 648 3600 876 359 159 2042 Fragments arranged by size: 3600 2042 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2041 2243 2639 4006 4843 5100 5777 Size: 429 6 202 396 1367 837 257 677 Cuts at: 5777 6925 7170 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1367 1148 837 677 429 396 257 245 202 6 NaeI GCC'GGC Cuts at: 2010 2010 Size: 7684 NcoI C'CATG_G Cuts at: 513 1065 2166 2385 2839 513 Size: 552 1101 219 454 5358 Fragments arranged by size: 5358 1101 552 454 219 NdeI CA'TA_TG Cuts at: 387 4624 387 Size: 4237 3447 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 7684 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7684 NotI GC'GGCC_GC Cuts at: 5131 5131 Size: 7684 NspI r_CATG'y Cuts at: 1132 1229 4216 5042 6587 1132 Size: 97 2987 826 1545 2229 Fragments arranged by size: 2987 2229 1545 826 97 PasI CC'CwG_GG Cuts at: 1348 2297 1348 Size: 949 6735 PciI A'CATG_T Cuts at: 6583 6583 Size: 7684 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7684 PmeI GTTT'AAAC Cuts at: 4988 4988 Size: 7684 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5753 5785 7266 7298 5753 Size: 32 1481 32 6139 Fragments arranged by size: 6139 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7684 PsiI TTA'TAA Cuts at: 3053 4766 5254 3053 Size: 1713 488 5483 Fragments arranged by size: 5483 1713 488 PspXI vC'TCGA_Gb Cuts at: 2879 2879 Size: 7684 PstI C_TGCA'G Cuts at: 830 5036 830 Size: 4206 3478 PvuI CG_AT'CG Cuts at: 2001 2583 2001 Size: 582 7102 PvuII CAG'CTG Cuts at: 2035 2639 4006 4843 2035 Size: 604 1367 837 4876 Fragments arranged by size: 4876 1367 837 604 SacI G_AGCT'C Cuts at: 2219 2219 Size: 7684 SalI G'TCGA_C Cuts at: 1936 5026 1936 Size: 3090 4594 SbfI CC_TGCA'GG Cuts at: 5036 5036 Size: 7684 ScaI AGT'ACT Cuts at: 3502 3770 5932 7644 3502 Size: 268 2162 1712 3542 Fragments arranged by size: 3542 2162 1712 268 SfcI C'TryA_G Cuts at: 826 1045 3731 4746 5032 6167 6848 7039 826 Size: 219 2686 1015 286 1135 681 191 1471 Fragments arranged by size: 2686 1471 1135 1015 681 286 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7684 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7684 SmaI CCC'GGG Cuts at: 1872 5011 1872 Size: 3139 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2879 3321 3989 4503 4792 5788 Size: 197 926 936 442 668 514 289 996 Cuts at: 5788 6689 6951 820 Size: 901 262 1553 Fragments arranged by size: 1553 996 936 926 901 668 514 442 289 262 197 SnaBI TAC'GTA Cuts at: 493 5475 493 Size: 4982 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7684 SphI G_CATG'C Cuts at: 1132 4216 5042 1132 Size: 3084 826 3774 Fragments arranged by size: 3774 3084 826 SspI AAT'ATT Cuts at: 5 52 2466 4024 4632 5608 5 Size: 47 2414 1558 608 976 2081 Fragments arranged by size: 2414 2081 1558 976 608 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7684 StyI C'CwwG_G Cuts at: 513 1065 2166 2385 2839 3562 5123 513 Size: 552 1101 219 454 723 1561 3074 Fragments arranged by size: 3074 1561 1101 723 552 454 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2581 3478 4478 5711 5728 5881 6066 Size: 1255 1001 897 1000 1233 17 153 185 Cuts at: 6066 6511 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1233 1001 1000 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3500 3699 3768 Size: 274 80 33 51 1443 1523 199 69 Cuts at: 3768 5930 7642 96 Size: 2162 1712 138 Fragments arranged by size: 2162 1712 1523 1443 274 199 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2678 2710 3474 3506 7644 7676 2678 Size: 32 764 32 4138 32 2686 Fragments arranged by size: 4138 2686 764 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7684 XbaI T'CTAG_A Cuts at: 5020 5020 Size: 7684 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2710 3384 3390 2011 Size: 699 674 6 6305 Fragments arranged by size: 6305 699 674 6 XhoI C'TCGA_G Cuts at: 1943 2879 4503 1943 Size: 936 1624 5124 Fragments arranged by size: 5124 1624 936 XmaI C'CCGG_G Cuts at: 1870 5009 1870 Size: 3139 4545 XmnI GAAnn'nnTTC Cuts at: 4131 4365 5813 4131 Size: 234 1448 6002 Fragments arranged by size: 6002 1448 234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5492 276 Size: 53 83 186 936 3958 2468 Fragments arranged by size: 3958 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI