(Circular) (Six-base) MAPSORT of: pFN21AE9683.seq Check: 3238 from: 1 to: 5941 pFN21AE9683 5941 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 2860 3262 2860 Size: 402 5539 AclI AA'CG_TT Cuts at: 1563 3240 4068 4441 1563 Size: 1677 828 373 3063 Fragments arranged by size: 3063 1677 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AfeI AGC'GCT Cuts at: 2088 2088 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2317 4840 5817 1203 Size: 1114 2523 977 1327 Fragments arranged by size: 2523 1327 1114 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 3684 5256 3684 Size: 1572 4369 ApaLI G'TGCA_C Cuts at: 2622 3998 5154 2622 Size: 1376 1156 3409 Fragments arranged by size: 3409 1376 1156 ApoI r'AATT_y Cuts at: 1493 2130 2260 2329 2386 2889 3001 3022 3102 Size: 637 130 69 57 503 112 21 80 Cuts at: 3102 3250 3476 3628 5737 1493 Size: 148 226 152 2109 1697 Fragments arranged by size: 2109 1697 637 503 226 152 148 130 112 80 69 57 21 AseI AT'TA_AT Cuts at: 160 1032 2178 4495 160 Size: 872 1146 2317 1606 Fragments arranged by size: 2317 1606 1146 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 3266 1870 Size: 73 1323 4545 Fragments arranged by size: 4545 1323 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2659 2692 2659 Size: 33 5908 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2860 3262 4717 618 Size: 325 217 627 1073 402 1455 1842 Fragments arranged by size: 1842 1455 1073 627 402 325 217 BanII G_rGCy'C Cuts at: 1391 2562 3260 1391 Size: 1171 698 4072 Fragments arranged by size: 4072 1171 698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2402 928 Size: 971 503 4467 Fragments arranged by size: 4467 971 503 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2518 2932 3833 5043 2518 Size: 414 901 1210 3416 Fragments arranged by size: 3416 1210 901 414 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2626 4002 5158 1790 Size: 836 1376 1156 2573 Fragments arranged by size: 2573 1376 1156 836 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3177 3672 4630 Size: 416 53 83 399 24 1760 495 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1760 1753 958 495 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3072 3104 4601 1239 Size: 1833 32 1497 2579 Fragments arranged by size: 2579 1833 1497 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 4066 4931 5229 2513 Size: 1553 865 298 3225 Fragments arranged by size: 3225 1553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 4604 882 Size: 617 547 2558 2219 Fragments arranged by size: 2558 2219 617 547 BsaAI yAC'GTr Cuts at: 493 1204 2318 2465 3732 493 Size: 711 1114 147 1267 2702 Fragments arranged by size: 2702 1267 1114 711 147 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 4374 4772 5046 5193 5604 2016 Size: 2358 398 274 147 411 2353 Fragments arranged by size: 2358 2353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2836 2866 1178 Size: 30 165 30 1433 30 4253 Fragments arranged by size: 4253 1433 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2765 1186 Size: 1579 4362 BseYI C'CCAG_C Cuts at: 2801 5144 5640 2801 Size: 2343 496 3102 Fragments arranged by size: 3102 2343 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2626 4002 4087 5158 2626 Size: 1376 85 1071 3409 Fragments arranged by size: 3409 1376 1071 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2060 2060 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2562 2626 3260 4002 4087 5158 1391 Size: 399 772 64 634 742 85 1071 2174 Fragments arranged by size: 2174 1071 772 742 634 399 85 64 BspEI T'CCGG_A Cuts at: 2016 5604 2016 Size: 3588 2353 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2497 3828 1394 Size: 429 674 1331 3507 Fragments arranged by size: 3507 1331 674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2339 4001 5013 2339 Size: 1662 1012 3267 Fragments arranged by size: 3267 1662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2303 2056 Size: 247 5694 BstYI r'GATC_y Cuts at: 1972 3031 3271 3638 4023 4040 5492 5578 5590 Size: 1059 240 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2544 3180 3457 4251 4271 2544 Size: 636 277 794 20 4214 Fragments arranged by size: 4214 794 636 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 2700 3000 3245 2700 Size: 300 245 5396 Fragments arranged by size: 5396 300 245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3388 4277 Size: 54 1425 50 275 18 216 1342 889 Cuts at: 4277 5906 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1342 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3043 3871 1353 Size: 1197 493 828 3423 Fragments arranged by size: 3423 1197 828 493 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3072 3104 4004 4601 5388 5656 1239 Size: 393 1440 32 900 597 787 268 1524 Fragments arranged by size: 1524 1440 900 787 597 393 268 32 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2090 5088 1678 Size: 412 2998 2531 Fragments arranged by size: 2998 2531 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2753 2785 3198 Size: 32 135 32 830 32 319 32 413 Cuts at: 3198 3230 4582 4614 4656 4688 1373 Size: 32 1352 32 42 32 2626 Fragments arranged by size: 2626 1352 830 413 319 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 2864 3266 2864 Size: 402 5539 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2802 5055 5239 1305 Size: 1497 2253 184 2007 Fragments arranged by size: 2253 2007 1497 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 3903 Fragments arranged by size: 3903 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 3899 4258 4417 518 Size: 648 2733 359 159 2042 Fragments arranged by size: 2733 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3357 4034 5182 5427 1606 Size: 1751 677 1148 245 2120 Fragments arranged by size: 2120 1751 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3015 3299 4844 1132 Size: 97 1786 284 1545 2229 Fragments arranged by size: 2229 1786 1545 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2821 1108 Size: 1713 4228 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2318 2318 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2499 2531 2499 Size: 32 5909 PstI C_TGCA'G Cuts at: 830 2082 3293 830 Size: 1252 1211 3478 Fragments arranged by size: 3478 1252 1211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SapI GCTCTTCn'nnn_ Cuts at: 2550 2550 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 2247 2604 4189 5901 2247 Size: 357 1585 1712 2287 Fragments arranged by size: 2287 1712 1585 357 SfcI C'TryA_G Cuts at: 826 1045 2078 3289 4424 5105 5296 826 Size: 219 1033 1211 1135 681 191 1471 Fragments arranged by size: 1471 1211 1135 1033 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 4045 4946 5208 820 Size: 197 926 549 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 549 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 2593 152 Size: 2441 3500 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 2182 3865 5 Size: 47 2130 1683 2081 Fragments arranged by size: 2130 2081 1683 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3196 3968 3985 4138 4323 4768 325 Size: 1255 1616 772 17 153 185 445 1498 Fragments arranged by size: 1616 1498 1255 772 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2245 2335 2602 Size: 274 80 33 51 1443 268 90 267 Cuts at: 2602 2873 4187 5899 96 Size: 271 1314 1712 138 Fragments arranged by size: 1712 1443 1314 274 271 268 267 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 2854 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 2388 3114 4070 2388 Size: 726 956 4259 Fragments arranged by size: 4259 956 726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI