################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 24 GACGTC GACGT^C AciI 235 CCGC CCGC(-3/-1) AfaI 112 GTAC GT^AC AflIII 74 ACACGT A^C[GA][CT]GT AluI 290 AGCT AG^CT AvaII 444 GGACC G^G[AT]CC BanII 473 GAGCCC G[GA]GC[CT]^C BcnI 264 CCGGG CC^[GC]GG BfaI 292 CTAG C^TAG BpmI 470 CTGGAG CTGGAG(16/14) BsaJI 51 CCTGGG C^C[ACGT][ACGT]GG BsmAI 215 GTCTC GTCTC(1/5) Bsp1407I 111 TGTACA T^GTACA BsrGI 111 TGTACA T^GTACA BssSI 187 CTCGTG CACGAG(-5/-1) BstNI 51 CCTGG CC^[AT]GG DpnII 318 GATC ^GATC DraII 15 AGGCCCC [GA]G^G[ACGT]CC[CT] EarI 172 GAAGAG CTCTTC(1/4) EcoO109I 15 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRII 51 CCTGG ^CC[AT]GG HapII 264 CCGG C^CGG HgaI 226 GACGC GACGC(5/10) HpaII 264 CCGG C^CGG Ksp632I 172 GAAGAG CTCTTC(1/4) MaeI 292 CTAG C^TAG MboI 318 GATC ^GATC MslI 369 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 264 CCGG C^CGG MvaI 51 CCTGG CC^[AT]GG NciI 264 CCGGG CC^[GC]GG NdeII 318 GATC ^GATC RsaI 112 GTAC GT^AC Sau3AI 318 GATC ^GATC SmlI 274 CTCAAG C^T[CT][GA]AG SspBI 111 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 24 GACGTC G[GA]^CG[CT]C 226 GACGCC BbvI 47 GCTGC GCAGC(8/12) 207 GCTGC BcgI 23 CGACGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 440 GCATGGACCTCG BsaHI 24 GACGTC G[GA]^CG[CT]C 226 GACGCC BsmFI 138 GTCCC GGGAC(10/14) 192 GTCCC BssKI 51 CCTGG ^CC[ACGT]GG 264 CCGGG Cfr13I 16 GGCCC G^G[ACGT]CC 444 GGACC DdeI 12 CTCAG C^T[ACGT]AG 217 CTCAG Hin1I 24 GACGTC G[GA]^CG[CT]C 226 GACGCC MaeII 25 ACGT A^CGT 76 ACGT MboII 147 GAAGA GAAGA(8/7) 172 GAAGA NlaIII 391 CATG CATG^ 441 CATG NlaIV 16 GGCCCC GG[ACGT]^[ACGT]CC 472 GGAGCC Sau96I 16 GGCCC G^G[ACGT]CC 444 GGACC ScrFI 51 CCTGG CC^[ACGT]GG 264 CCGGG SfaNI 150 GATGC GCATC(5/9) 352 GATGC TseI 47 GCTGC G^C[AT]GC 207 GCTGC Tsp509I 131 AATT ^AATT 199 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AspHI BglI BmyI BsiHKAI Bsp1286I BstF5I Cac8I Fnu4HI FokI HaeIII ItaI MnlI MwoI TaqI TthHB8I