Restriction map for
pFN21AE1810
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsgI BsiWI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TspRI TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 53 CGCG CG^CG
AccIII 656 TCCGGA T^CCGGA
AhdI 829 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 422 GGGCCC GGGCC^C
AspEI 829 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 171 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 956 TGGCCA TGG^CCA
BamHI 653 GGATCC G^GATCC
BanII 422 GGGCCC G[GA]GC[CT]^C
BclI 675 TGATCA T^GATCA
BsaI 491 GGTCTC GGTCTC(1/5)
BseAI 656 TCCGGA T^CCGGA
BsiEI 39 CGGCCG CG[GA][CT]^CG
BsmBI 875 CGTCTC CGTCTC(1/5)
BsmI 361 GCATTC GAATGC(1/-1)
BspEI 656 TCCGGA T^CCGGA
BsrI 830 ACTGG ACTGG(1/-1)
BssSI 494 CTCGTG CACGAG(-5/-1)
BstF5I 798 CATCC GGATG(2/0)
BstUI 53 CGCG CG^CG
BstYI 653 GGATCC [GA]^GATC[CT]
DraIII 919 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 42 CCGTGG C^C[GA][CT]GG
EagI 39 CGGCCG C^GGCCG
Eam1105I 829 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 39 CGGCCG C^GGCCG
Eco52I 39 CGGCCG C^GGCCG
EcoNI 807 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 675 TGATCA T^GATCA
FokI 798 CATCC GGATG(9/13)
MflI 653 GGATCC [GA]^GATC[CT]
MluNI 956 TGGCCA TGG^CCA
MroI 656 TCCGGA T^CCGGA
MscI 956 TGGCCA TGG^CCA
MvnI 53 CGCG CG^CG
PflFI 171 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 258 GAGTC GAGTC(4/5)
PvuII 325 CAGCTG CAG^CTG
SmlI 557 CTCAAG C^T[CT][GA]AG
TfiI 478 GATTC G^A[AT]TC
Tth111I 171 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 653 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 432 AAATTT [GA]^AATT[CT]
736 GAATTT
AfaI 462 GTAC GT^AC
846 GTAC
AlwI 654 GATCC GGATC(4/5)
653 GGATC
ApoI 432 AAATTT [GA]^AATT[CT]
736 GAATTT
AspHI 20 GTGCTC G[AT]GC[AT]^C
116 GTGCTC
AvaII 105 GGACC G^G[AT]CC
320 GGACC
BsaWI 365 TCCGGT [AT]^CCGG[AT]
656 TCCGGA
BseRI 76 CTCCTC GAGGAG(10/8)
184 GAGGAG
BsiHKAI 20 GTGCTC G[AT]GC[AT]^C
116 GTGCTC
BsrDI 357 CATTGC GCAATG(2/0)
642 CATTGC
EaeI 39 CGGCCG [CT]^GGCC[GA]
956 TGGCCA
EcoT14I 482 CCTTGG C^C[AT][AT]GG
551 CCTTGG
HinfI 258 GAGTC G^A[ACGT]TC
478 GATTC
HphI 352 GGTGA GGTGA(8/7)
924 GGTGA
MseI 374 TTAA T^TAA
418 TTAA
MspA1I 146 CAGCGG C[AC]G^C[GT]G
325 CAGCTG
NlaIII 581 CATG CATG^
897 CATG
PpuMI 104 GGGACCT [GA]G^G[AT]CC[CT]
319 GGGACCC
RsaI 462 GTAC GT^AC
846 GTAC
SmaI 10 CCCGGG CCC^GGG
100 CCCGGG
StyI 482 CCTTGG C^C[AT][AT]GG
551 CCTTGG
Tru9I 374 TTAA T^TAA
418 TTAA
XmaCI 10 CCCGGG C^CCGGG
100 CCCGGG
XmaI 10 CCCGGG C^CCGGG
100 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaI BbvI BciVI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I