Restriction map for
pFN21AE1810
(Circular) (Six-base) MAPSORT of: pFN21AE1810.seq Check: 3087 from: 1 to: 5686
pFN21AE1810 5686 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 3749 5133 5401 1632
Size: 993 1124 1384 268 1917
Fragments arranged by size:
1917 1384 1124 993 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 2839 4415 2839
Size: 1576 4110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaI G_GGCC'C
Cuts at: 2431 2431
Size: 5686
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2437 2741 2995 3221 3373 5482 1493
Size: 944 304 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 944 304 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2084 2105 3011 1870
Size: 73 72 69 21 906 4545
Fragments arranged by size:
4545 906 73 72 69 21
BamHI G'GATC_C
Cuts at: 2658 3016 3383 2658
Size: 358 367 4961
Fragments arranged by size:
4961 367 358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2431 3005 1391
Size: 1040 574 4072
Fragments arranged by size:
4072 1040 574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2282 2561 2876 3578 4788 2282
Size: 279 315 702 1210 3180
Fragments arranged by size:
3180 1210 702 315 279
BclI T'GATC_A
Cuts at: 1548 2680 1548
Size: 1132 4554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4297 136
Size: 107 122 71 631 605 337 2288 1525
Fragments arranged by size:
2288 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2431 3747 4903 1790
Size: 641 1316 1156 2573
Fragments arranged by size:
2573 1316 1156 641
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 2043
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2675 2850 4346 1239
Size: 1128 308 175 1496 2579
Fragments arranged by size:
2579 1496 1128 308 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2547 3811 4676 4974 2547
Size: 1264 865 298 3259
Fragments arranged by size:
3259 1264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2502 4349 882
Size: 617 1003 1847 2219
Fragments arranged by size:
2219 1847 1003 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2370 2661 4119 4517 4791 4938 5349 2370
Size: 291 1458 398 274 147 411 2707
Fragments arranged by size:
2707 1458 411 398 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2204 1186
Size: 886 132 4668
Fragments arranged by size:
4668 886 132
BseYI C'CCAG_C
Cuts at: 2328 2394 4889 5385 2328
Size: 66 2495 496 2629
Fragments arranged by size:
2629 2495 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 3136 3897 4925 1540
Size: 275 186 46 1089 761 1028 2301
Fragments arranged by size:
2301 1089 1028 761 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 2029 2125 3747 3832 4903 2029
Size: 96 1622 85 1071 2812
Fragments arranged by size:
2812 1622 1071 96 85
BsmI GAATG_Cn'
Cuts at: 2366 3197 3290 2366
Size: 831 93 4762
Fragments arranged by size:
4762 831 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2886 2886
Size: 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2125 2431 3005 3747 3832 4903
Size: 399 239 96 306 574 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 574 399 306 239 96
85
BspEI T'CCGG_A
Cuts at: 2661 5349 2661
Size: 2688 2998
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 2646 4181 4355 65
Size: 1183 1113 285 1535 174 1396
Fragments arranged by size:
1535 1396 1183 1113 285 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 2499 3746 4758 2499
Size: 1247 1012 3427
Fragments arranged by size:
3427 1247 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2658 3016 3383 3768 3785 5237 5323 5335
Size: 686 358 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 686 385 367 358 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 513
Size: 552 939 43 4152
Fragments arranged by size:
4152 939 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2078 2809 487
Size: 683 56 852 731 3364
Fragments arranged by size:
3364 852 731 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3996 4016 3202
Size: 794 20 4872
Fragments arranged by size:
4872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DraIII CAC_nnn'GTG
Cuts at: 2929 2929
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2961 3133
Size: 54 1425 50 275 18 214 917 172
Cuts at: 3133 4022 5651 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 917 889 275 214 172 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 3133 1537
Size: 275 232 1089 4090
Fragments arranged by size:
4090 1089 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2408 2883 3616 1353
Size: 828 227 475 733 3423
Fragments arranged by size:
3423 828 733 475 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2625 2675 2850 3749 4346 5133
Size: 393 735 258 50 175 899 597 787
Cuts at: 5133 5401 1239
Size: 268 1524
Fragments arranged by size:
1524 899 787 735 597 393 268 258
175 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2816 2816
Size: 5686
EcoO109I rG'GnC_Cy
Cuts at: 2110 2325 2427 2110
Size: 215 102 5369
Fragments arranged by size:
5369 215 102
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2715 2747 2808
Size: 32 135 32 593 32 518 32 61
Cuts at: 2808 2825 2840 2857 4327 4359 4401 4433 1373
Size: 17 15 17 1470 32 42 32 2626
Fragments arranged by size:
2626 1470 593 518 135 61 42 32
32 32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2521 4800 4984 1305
Size: 858 358 2279 184 2007
Fragments arranged by size:
2279 2007 858 358 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2963 10
Size: 54 1425 343 1131 2733
Fragments arranged by size:
2733 1425 1131 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4003 4162 518
Size: 648 2478 359 159 2042
Fragments arranged by size:
2478 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2153 2332 3102 3779 4927 5172 1606
Size: 547 179 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 547 245 179
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4195
Fragments arranged by size:
4195 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2325 2110
Size: 215 5471
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2427 2427
Size: 5686
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SanDI GG'GwC_CC
Cuts at: 2325 2325
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 3034 4169 4850 5041 826
Size: 219 1989 1135 681 191 1471
Fragments arranged by size:
1989 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 2017 2107 3013 1872
Size: 145 90 906 4545
Fragments arranged by size:
4545 906 145 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 3790 4691 4953 820
Size: 197 926 619 1228 901 262 1553
Fragments arranged by size:
1553 1228 926 901 619 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 2556 3125 513
Size: 552 939 483 69 569 3074
Fragments arranged by size:
3074 939 569 552 483 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 3713 3730 3883 4068 4513 325
Size: 1255 460 1673 17 153 185 445 1498
Fragments arranged by size:
1673 1498 1255 460 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 2179 1316
Size: 863 4823
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2015 2105 3011 1870
Size: 145 90 906 4545
Fragments arranged by size:
4545 906 145 90
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI