Restriction map for
pFN21AE1810
(Linear) (Six-base) MAPSORT of: KIEE1810.seq Check: 5500 from: 1 to: 981
KIEE1810.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 981
Size: 620 361
AhdI GACnn_n'nnGTC
Cuts at: 0 834 981
Size: 834 147
ApaI G_GGCC'C
Cuts at: 0 426 981
Size: 426 555
ApoI r'AATT_y
Cuts at: 0 432 736 981
Size: 432 304 245
Fragments arranged by size:
432 304 245
AvaI C'yCGr_G
Cuts at: 0 10 79 100 981
Size: 10 69 21 881
Fragments arranged by size:
881 69 21 10
BamHI G'GATC_C
Cuts at: 0 653 981
Size: 653 328
BanII G_rGCy'C
Cuts at: 0 426 981
Size: 426 555
BciVI GTATCCnnnnn_n'
Cuts at: 0 277 556 871 981
Size: 277 279 315 110
Fragments arranged by size:
315 279 277 110
BclI T'GATC_A
Cuts at: 0 675 981
Size: 675 306
Bme1580I G_kGCm'C
Cuts at: 0 426 981
Size: 426 555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 38 981
Size: 38 943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 670 845 981
Size: 362 308 175 136
Fragments arranged by size:
362 308 175 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 981
Size: 542 439
BsaI GGTCTCn'nnnn_
Cuts at: 0 497 981
Size: 497 484
BsaWI w'CCGG_w
Cuts at: 0 365 656 981
Size: 365 291 325
Fragments arranged by size:
365 325 291
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 199 981
Size: 67 132 782
Fragments arranged by size:
782 132 67
BseYI C'CCAG_C
Cuts at: 0 323 389 981
Size: 323 66 592
Fragments arranged by size:
592 323 66
BsiEI CG_ry'CG
Cuts at: 0 42 981
Size: 42 939
BsiHKAI G_wGCw'C
Cuts at: 0 24 120 981
Size: 24 96 861
Fragments arranged by size:
861 96 24
BsmI GAATG_Cn'
Cuts at: 0 361 981
Size: 361 620
BsmBI CGTCTCn'nnnn_
Cuts at: 0 881 981
Size: 881 100
Bsp1286I G_dGCh'C
Cuts at: 0 24 120 426 981
Size: 24 96 306 555
Fragments arranged by size:
555 306 96 24
BspEI T'CCGG_A
Cuts at: 0 656 981
Size: 656 325
BsrDI GCAATG_nn'
Cuts at: 0 356 641 981
Size: 356 285 340
Fragments arranged by size:
356 340 285
BssSI C'ACGA_G
Cuts at: 0 494 981
Size: 494 487
BstYI r'GATC_y
Cuts at: 0 653 981
Size: 653 328
BtgI C'CryG_G
Cuts at: 0 42 981
Size: 42 939
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 73 804 981
Size: 73 731 177
Fragments arranged by size:
731 177 73
DraIII CAC_nnn'GTG
Cuts at: 0 924 981
Size: 924 57
EaeI y'GGCC_r
Cuts at: 0 39 956 981
Size: 39 917 25
Fragments arranged by size:
917 39 25
EagI C'GGCC_G
Cuts at: 0 39 981
Size: 39 942
EarI CTCTTCn'nnn_
Cuts at: 0 176 403 878 981
Size: 176 227 475 103
Fragments arranged by size:
475 227 176 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 620 670 845 981
Size: 362 258 50 175 136
Fragments arranged by size:
362 258 175 136 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 811 981
Size: 811 170
EcoO109I rG'GnC_Cy
Cuts at: 0 105 320 422 981
Size: 105 215 102 559
Fragments arranged by size:
559 215 105 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 710 742 803 820 835 852
Size: 160 32 518 32 61 17 15 17
Cuts at: 852 981
Size: 129
Fragments arranged by size:
518 160 129 61 32 32 17 17
15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 516 981
Size: 158 358 465
Fragments arranged by size:
465 358 158
MscI TGG'CCA
Cuts at: 0 958 981
Size: 958 23
MspA1I CmG'CkG
Cuts at: 0 148 327 981
Size: 148 179 654
Fragments arranged by size:
654 179 148
PfoI T'CCnGG_A
Cuts at: 0 686 981
Size: 686 295
PpuMI rG'GwC_Cy
Cuts at: 0 105 320 981
Size: 105 215 661
Fragments arranged by size:
661 215 105
PspOMI G'GGCC_C
Cuts at: 0 422 981
Size: 422 559
PvuII CAG'CTG
Cuts at: 0 327 981
Size: 327 654
SanDI GG'GwC_CC
Cuts at: 0 320 981
Size: 320 661
SmaI CCC'GGG
Cuts at: 0 12 102 981
Size: 12 90 879
Fragments arranged by size:
879 90 12
SmlI C'TyrA_G
Cuts at: 0 557 981
Size: 557 424
StyI C'CwwG_G
Cuts at: 0 482 551 981
Size: 482 69 430
Fragments arranged by size:
482 430 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 981
Size: 35 946
Tth111I GACn'n_nGTC
Cuts at: 0 174 981
Size: 174 807
XmaI C'CCGG_G
Cuts at: 0 10 100 981
Size: 10 90 881
Fragments arranged by size:
881 90 10
Enzymes that do cut:
AcuI AhdI ApaI ApoI AvaI BamHI BanII BciVI
BclI Bme1580I BplI BpmI BpuEI BsaI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI
BssSI BstYI BtgI BtgZI DraIII EaeI EagI EarI
Eco57MI EcoNI EcoO109I Hin4I MmeI MscI MspA1I PfoI
PpuMI PspOMI PvuII SanDI SmaI SmlI StyI TaqII
Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsaHI BsaXI BsgI BsiWI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI XbaI XcmI XhoI
XmnI ZraI