Restriction map for
pFN21AE2065
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# NO CUT ENZYMES ( 124 enzymes) #
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AccI AccII AclI AcsI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BglI BglII BlnI BsaAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KspI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 127 GACGTC GACGT^C
Acc65I 223 GGTACC G^GTACC
AccIII 1775 TCCGGA T^CCGGA
AflII 204 CTTAAG C^TTAAG
Asp718I 223 GGTACC G^GTACC
AspHI 484 GAGCTC G[AT]GC[AT]^C
BalI 170 TGGCCA TGG^CCA
BbsI 1209 GAAGAC GAAGAC(2/6)
BcgI 1500 CGAGGTTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 761 TGATCA T^GATCA
BfrI 204 CTTAAG C^TTAAG
BlpI 1392 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1392 GCTGAGC GC^T[ACGT]AGC
BpuAI 1209 GAAGAC GAAGAC(2/6)
BsaBI 1222 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1775 TCCGGA [AT]^CCGG[AT]
BseAI 1775 TCCGGA T^CCGGA
BsiHKAI 484 GAGCTC G[AT]GC[AT]^C
BspEI 1775 TCCGGA T^CCGGA
BspMI 984 ACCTGC ACCTGC(4/8)
BsrBI 1279 GAGCGG CCGCTC(-3/-3)
CelII 1392 GCTGAGC GC^T[ACGT]AGC
DraIII 330 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 761 TGATCA T^GATCA
HgaI 740 GCGTC GACGC(5/10)
HpaI 1108 GTTAAC GTT^AAC
KpnI 223 GGTACC GGTAC^C
MamI 1222 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 170 TGGCCA TGG^CCA
MroI 1775 TCCGGA T^CCGGA
MscI 170 TGGCCA TGG^CCA
MslI 384 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 1306 CATG CATG^
PleI 13 GAGTC GAGTC(4/5)
PshAI 568 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 484 GAGCTC GAGCT^C
SbfI 132 CCTGCAGG CCTGCA^GG
SexAI 949 ACCTGGT A^CC[AT]GGT
SmaI 1545 CCCGGG CCC^GGG
SmlI 204 CTTAAG C^T[CT][GA]AG
Sse8387I 132 CCTGCAGG CCTGCA^GG
StuI 1894 AGGCCT AGG^CCT
XmaCI 1545 CCCGGG C^CCGGG
XmaI 1545 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 127 GACGTC G[GA]^CG[CT]C
739 GGCGTC
AvaI 1498 CCCGAG C^[CT]CG[GA]G
1545 CCCGGG
BamHI 876 GGATCC G^GATCC
2081 GGATCC
BanI 30 GGTGCC G^G[CT][GA]CC
223 GGTACC
BanII 484 GAGCTC G[GA]GC[CT]^C
1513 GGGCTC
BfaI 1087 CTAG C^TAG
2078 CTAG
BmyI 484 GAGCTC G[AGT]GC[ACT]^C
1513 GGGCTC
BsaHI 127 GACGTC G[GA]^CG[CT]C
739 GGCGTC
BsaI 820 GAGACC GGTCTC(1/5)
1852 GAGACC
BsoBI 1498 CCCGAG C^[CT]CG[GA]G
1545 CCCGGG
Bsp1286I 484 GAGCTC G[AGT]GC[ACT]^C
1513 GGGCTC
BsrDI 729 CATTGC GCAATG(2/0)
924 GCAATG
EaeI 170 TGGCCA [CT]^GGCC[GA]
428 TGGCCG
EcoNI 156 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1322 CCTTTTGGAGG
Hin1I 127 GACGTC G[GA]^CG[CT]C
739 GGCGTC
MaeI 1087 CTAG C^TAG
2078 CTAG
PflMI 464 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACCTTCTGG
TfiI 410 GAATC G^A[AT]TC
2126 GAATC
Van91I 464 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACCTTCTGG
XcmI 1922 CCACAGGTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2026 CCAATACCACCTTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
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AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII