(Circular) (Six-base) MAPSORT of: pFN21AE2065.seq Check: 1361 from: 1 to: 6862 pFN21AE2065 6862 bp With 182 enzymes: SgfI * July 14, 2018 22:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2136 4672 278 Size: 53 83 186 936 600 2536 2468 Fragments arranged by size: 2536 2468 936 600 186 83 53 AccI GT'mk_AC Cuts at: 1937 4205 1937 Size: 2268 4594 Acc65I G'GTAC_C Cuts at: 2228 4183 2228 Size: 1955 4907 AclI AA'CG_TT Cuts at: 1563 4989 5362 1563 Size: 3426 373 3063 Fragments arranged by size: 3426 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2230 2339 2760 3233 3695 4925 6309 Size: 464 134 109 421 473 462 1230 1384 Cuts at: 6309 6577 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1230 473 464 462 421 268 134 109 AflII C'TTAA_G Cuts at: 820 1017 2209 820 Size: 197 1192 5473 Fragments arranged by size: 5473 1192 197 AflIII A'CryG_T Cuts at: 1203 5761 6738 1203 Size: 4558 977 1327 Fragments arranged by size: 4558 1327 977 AgeI A'CCGG_T Cuts at: 5693 5693 Size: 6862 AhdI GACnn_n'nnGTC Cuts at: 5591 5591 Size: 6862 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6862 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6862 AlwNI CAG_nnn'CTG Cuts at: 2050 2101 2475 4605 6177 2050 Size: 51 374 2130 1572 2735 Fragments arranged by size: 2735 2130 1572 374 51 ApaLI G'TGCA_C Cuts at: 4919 6075 4919 Size: 1156 5706 ApoI r'AATT_y Cuts at: 1493 4171 4397 4549 6658 1493 Size: 2678 226 152 2109 1697 Fragments arranged by size: 2678 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5416 160 Size: 872 4384 1606 Fragments arranged by size: 4384 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 AvaI C'yCGr_G Cuts at: 1870 1943 3503 3550 4187 1870 Size: 73 1560 47 637 4545 Fragments arranged by size: 4545 1560 637 73 47 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2220 2253 2220 Size: 33 6829 BamHI G'GATC_C Cuts at: 2881 4086 4192 4559 2881 Size: 1205 106 367 5184 Fragments arranged by size: 5184 1205 367 106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2228 4183 5638 618 Size: 325 217 627 248 193 1955 1455 1842 Fragments arranged by size: 1955 1842 1455 627 325 248 217 193 BanII G_rGCy'C Cuts at: 1391 2493 3522 4181 1391 Size: 1102 1029 659 4072 Fragments arranged by size: 4072 1102 1029 659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3221 928 Size: 971 1322 4569 Fragments arranged by size: 4569 1322 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3494 3528 5053 5087 1903 Size: 34 1557 34 1525 34 3678 Fragments arranged by size: 3678 1557 1525 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4754 5964 4754 Size: 1210 5652 BclI T'GATC_A Cuts at: 1548 2766 1548 Size: 1218 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5473 136 Size: 107 122 71 631 605 3801 1525 Fragments arranged by size: 3801 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6857 6857 Size: 6862 BlpI GC'TnA_GC Cuts at: 3398 4279 3398 Size: 881 5981 Bme1580I G_kGCm'C Cuts at: 1790 4923 6079 1790 Size: 3133 1156 2573 Fragments arranged by size: 3133 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3937 1318 Size: 2619 4243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 3479 4593 Size: 416 53 83 399 24 899 1163 1114 Cuts at: 4593 5551 442 Size: 958 1753 Fragments arranged by size: 1753 1163 1114 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6862 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3423 4129 3423 Size: 706 6156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2540 2699 2780 3056 3812 5522 1239 Size: 1301 159 81 276 756 1710 2579 Fragments arranged by size: 2579 1710 1301 756 276 159 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4987 5852 6150 4987 Size: 865 298 5699 Fragments arranged by size: 5699 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2819 3851 5525 882 Size: 617 1320 1032 1674 2219 Fragments arranged by size: 2219 1674 1320 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 4653 493 Size: 711 3449 2702 Fragments arranged by size: 3449 2702 711 BsaBI GATnn'nnATC Cuts at: 3231 6856 3231 Size: 3625 3237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2745 4669 5051 Size: 53 83 186 936 600 612 1924 382 Cuts at: 5051 5686 275 Size: 635 1451 Fragments arranged by size: 1924 1451 936 635 612 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3780 5295 5693 5967 6114 6525 3780 Size: 1515 398 274 147 411 4117 Fragments arranged by size: 4117 1515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 1178 Size: 30 165 30 1368 30 5239 Fragments arranged by size: 5239 1368 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3359 3362 3570 1186 Size: 2173 3 208 4478 Fragments arranged by size: 4478 2173 208 3 BseYI C'CCAG_C Cuts at: 2022 2050 2468 3497 3916 6065 6561 2022 Size: 28 418 1029 419 2149 496 2323 Fragments arranged by size: 2323 2149 1029 496 419 418 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2734 2831 2976 3000 1163 Size: 573 450 548 97 145 24 5025 Fragments arranged by size: 5025 573 548 450 145 97 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4312 5073 6101 1540 Size: 275 186 2311 761 1028 2301 Fragments arranged by size: 2311 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 4923 5008 6079 2493 Size: 2430 85 1071 3276 Fragments arranged by size: 3276 2430 1071 85 BsmI GAATG_Cn' Cuts at: 4373 4466 4373 Size: 93 6769 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 3522 4181 4923 5008 6079 1391 Size: 399 703 1029 659 742 85 1071 2174 Fragments arranged by size: 2174 1071 1029 742 703 659 399 85 BspEI T'CCGG_A Cuts at: 3780 6525 3780 Size: 2745 4117 BspHI T'CATG_A Cuts at: 4751 6481 4751 Size: 1730 5132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2998 4217 844 Size: 2154 1219 3489 Fragments arranged by size: 3489 2154 1219 BsrBI CCG'CTC Cuts at: 1394 1823 3286 4749 1394 Size: 429 1463 1463 3507 Fragments arranged by size: 3507 1463 1463 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2733 2936 5357 5531 65 Size: 1183 1485 203 2421 174 1396 Fragments arranged by size: 2421 1485 1396 1183 203 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5506 5693 1700 Size: 110 3696 187 2869 Fragments arranged by size: 3696 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6862 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6862 BssSI C'ACGA_G Cuts at: 4922 5934 4922 Size: 1012 5850 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6862 BstBI TT'CG_AA Cuts at: 5666 5666 Size: 6862 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6862 BstYI r'GATC_y Cuts at: 1972 2361 2478 2498 2881 3168 4086 4192 4559 Size: 389 117 20 383 287 918 106 367 Cuts at: 4559 4944 4961 6413 6499 6511 6857 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 918 389 385 383 367 346 287 117 106 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5371 Fragments arranged by size: 5371 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6123 Fragments arranged by size: 6123 683 56 BtsI GCAGTG_nn' Cuts at: 3765 4378 5172 5192 3765 Size: 613 794 20 5435 Fragments arranged by size: 5435 794 613 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6862 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6827 DraI TTT'AAA Cuts at: 4166 4166 Size: 6862 DraIII CAC_nnn'GTG Cuts at: 2340 2340 Size: 6862 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5869 809 Size: 507 4553 1802 Fragments arranged by size: 4553 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2433 4309 Size: 54 1425 50 275 18 345 258 1876 Cuts at: 4309 5198 6827 8 Size: 889 1629 43 Fragments arranged by size: 1876 1629 1425 889 345 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4309 1537 Size: 275 2497 4090 Fragments arranged by size: 4090 2497 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 2270 3029 4792 1353 Size: 893 24 759 1763 3423 Fragments arranged by size: 3423 1763 893 759 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5449 5823 5969 387 Size: 1064 138 3860 374 146 1280 Fragments arranged by size: 3860 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2491 2491 Size: 6862 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2230 2339 2540 2699 2760 2780 Size: 393 464 134 109 201 159 61 20 Cuts at: 2780 3056 3233 3695 3812 4925 5522 6309 6577 Size: 276 177 462 117 1113 597 787 268 Cuts at: 6577 1239 Size: 1524 Fragments arranged by size: 1524 1113 787 597 464 462 393 276 268 201 177 159 134 117 109 61 20 EcoNI CCTnn'n_nnAGG Cuts at: 2165 3331 2165 Size: 1166 5696 EcoRI G'AATT_C Cuts at: 4171 4549 4171 Size: 378 6484 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3724 3724 Size: 6862 FspI TGC'GCA Cuts at: 5368 5368 Size: 6862 HaeII r_GCGC'y Cuts at: 1678 6009 1678 Size: 4331 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2642 2674 4071 4103 5503 Size: 32 135 32 1070 32 1397 32 1400 Cuts at: 5503 5535 5577 5609 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1400 1397 1070 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2806 2890 2961 3115 4206 4452 Size: 988 281 868 84 71 154 1091 246 Cuts at: 4452 669 Size: 3079 Fragments arranged by size: 3079 1091 988 868 281 246 154 84 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6862 HpaI GTT'AAC Cuts at: 3115 4452 3115 Size: 1337 5525 KpnI G_GTAC'C Cuts at: 2232 4187 2232 Size: 1955 4907 MfeI C'AATT_G Cuts at: 4461 4461 Size: 6862 MluI A'CGCG_T Cuts at: 6738 6738 Size: 6862 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3175 5976 6160 1305 Size: 1870 2801 184 2007 Fragments arranged by size: 2801 2007 1870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 4695 Fragments arranged by size: 4695 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 4820 5179 5338 518 Size: 648 1227 2427 359 159 2042 Fragments arranged by size: 2427 2042 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 3319 3478 4278 4955 6103 6348 1606 Size: 420 1293 159 800 677 1148 245 2120 Fragments arranged by size: 2120 1293 1148 800 677 420 245 159 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5371 Fragments arranged by size: 5371 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6862 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6862 NotI GC'GGCC_GC Cuts at: 4309 4309 Size: 6862 NspI r_CATG'y Cuts at: 1132 1229 4220 5765 1132 Size: 97 2991 1545 2229 Fragments arranged by size: 2991 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3390 1348 Size: 2042 4820 PciI A'CATG_T Cuts at: 5761 5761 Size: 6862 PflMI CCAn_nnn'nTGG Cuts at: 1108 2475 3046 1108 Size: 1367 571 4924 Fragments arranged by size: 4924 1367 571 PfoI T'CCnGG_A Cuts at: 2064 2265 2581 3789 2064 Size: 201 316 1208 5137 Fragments arranged by size: 5137 1208 316 201 PmeI GTTT'AAAC Cuts at: 4166 4166 Size: 6862 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4931 4963 6444 6476 4931 Size: 32 1481 32 5317 Fragments arranged by size: 5317 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2577 1613 Size: 964 5898 PsiI TTA'TAA Cuts at: 4432 4432 Size: 6862 PstI C_TGCA'G Cuts at: 830 2142 3588 3772 4214 830 Size: 1312 1446 184 442 3478 Fragments arranged by size: 3478 1446 1312 442 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6862 PvuII CAG'CTG Cuts at: 2026 3319 3478 2026 Size: 1293 159 5410 Fragments arranged by size: 5410 1293 159 SacI G_AGCT'C Cuts at: 2493 2493 Size: 6862 SalI G'TCGA_C Cuts at: 1936 4204 1936 Size: 2268 4594 SbfI CC_TGCA'GG Cuts at: 2142 4214 2142 Size: 2072 4790 ScaI AGT'ACT Cuts at: 5110 6822 5110 Size: 1712 5150 SexAI A'CCwGG_T Cuts at: 2954 2954 Size: 6862 SfcI C'TryA_G Cuts at: 826 1045 2138 3584 3768 4210 5345 6026 6217 Size: 219 1093 1446 184 442 1135 681 191 Cuts at: 6217 826 Size: 1471 Fragments arranged by size: 1471 1446 1135 1093 681 442 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6862 SmaI CCC'GGG Cuts at: 1872 3552 4189 1872 Size: 1680 637 4545 Fragments arranged by size: 4545 1680 637 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 4966 5867 6129 820 Size: 197 926 266 2757 901 262 1553 Fragments arranged by size: 2757 1553 926 901 266 262 197 SnaBI TAC'GTA Cuts at: 493 4653 493 Size: 4160 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6862 SphI G_CATG'C Cuts at: 1132 4220 1132 Size: 3088 3774 SspI AAT'ATT Cuts at: 5 52 4786 5 Size: 47 4734 2081 Fragments arranged by size: 4734 2081 47 StuI AGG'CCT Cuts at: 1517 3901 1517 Size: 2384 4478 StyI C'CwwG_G Cuts at: 513 1065 2004 2502 3708 4040 4301 513 Size: 552 939 498 1206 332 261 3074 Fragments arranged by size: 3074 1206 939 552 498 332 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3511 3929 4889 4906 5059 5244 5689 Size: 1255 1931 418 960 17 153 185 445 Cuts at: 5689 325 Size: 1498 Fragments arranged by size: 1931 1498 1255 960 445 418 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5108 6820 96 Size: 274 80 33 51 1443 3131 1712 138 Fragments arranged by size: 3131 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 2771 2803 3941 3973 6822 6854 2696 Size: 32 43 32 1138 32 2849 32 2704 Fragments arranged by size: 2849 2704 1138 43 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6862 XbaI T'CTAG_A Cuts at: 4198 4198 Size: 6862 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3934 4038 3934 Size: 104 6758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6862 XmaI C'CCGG_G Cuts at: 1870 3550 4187 1870 Size: 1680 637 4545 Fragments arranged by size: 4545 1680 637 XmnI GAAnn'nnTTC Cuts at: 4991 4991 Size: 6862 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2134 4670 276 Size: 53 83 186 936 600 2536 2468 Fragments arranged by size: 2536 2468 936 600 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI