Restriction map for
pFN21AE2065
(Linear) (Six-base) MAPSORT of: KIEE2065.seq Check: 9602 from: 1 to: 2157
KIEE2065.orf
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AatII G_ACGT'C
Cuts at: 0 131 2157
Size: 131 2026
Acc65I G'GTAC_C
Cuts at: 0 223 2157
Size: 223 1934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 225 334 755 1228 1690 2157
Size: 91 134 109 421 473 462 467
Fragments arranged by size:
473 467 462 421 134 109 91
AflII C'TTAA_G
Cuts at: 0 204 2157
Size: 204 1953
AlwNI CAG_nnn'CTG
Cuts at: 0 45 96 470 2157
Size: 45 51 374 1687
Fragments arranged by size:
1687 374 51 45
AvaI C'yCGr_G
Cuts at: 0 1498 1545 2157
Size: 1498 47 612
Fragments arranged by size:
1498 612 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 2157
Size: 215 33 1909
Fragments arranged by size:
1909 215 33
BamHI G'GATC_C
Cuts at: 0 876 2081 2157
Size: 876 1205 76
Fragments arranged by size:
1205 876 76
BanI G'GyrC_C
Cuts at: 0 30 223 2157
Size: 30 193 1934
Fragments arranged by size:
1934 193 30
BanII G_rGCy'C
Cuts at: 0 488 1517 2157
Size: 488 1029 640
Fragments arranged by size:
1029 640 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 1216 2157
Size: 1216 941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1489 1523 2157
Size: 1489 34 634
Fragments arranged by size:
1489 634 34
BclI T'GATC_A
Cuts at: 0 761 2157
Size: 761 1396
BlpI GC'TnA_GC
Cuts at: 0 1393 2157
Size: 1393 764
BmgBI CAC'GTC
Cuts at: 0 1932 2157
Size: 1932 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 1474 2157
Size: 311 1163 683
Fragments arranged by size:
1163 683 311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1418 2124 2157
Size: 1418 706 33
Fragments arranged by size:
1418 706 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 694 775 1051 1807 2157
Size: 535 159 81 276 756 350
Fragments arranged by size:
756 535 350 276 159 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 814 1846 2157
Size: 814 1032 311
Fragments arranged by size:
1032 814 311
BsaBI GATnn'nnATC
Cuts at: 0 1226 2157
Size: 1226 931
BsaHI Gr'CG_yC
Cuts at: 0 128 740 2157
Size: 128 612 1417
Fragments arranged by size:
1417 612 128
BsaWI w'CCGG_w
Cuts at: 0 1775 2157
Size: 1775 382
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 2157
Size: 766 30 1361
Fragments arranged by size:
1361 766 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1354 1357 1565 2157
Size: 1354 3 208 592
Fragments arranged by size:
1354 592 208 3
BseYI C'CCAG_C
Cuts at: 0 17 45 463 1492 1911 2157
Size: 17 28 418 1029 419 246
Fragments arranged by size:
1029 419 418 246 28 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 729 826 971 995 2157
Size: 181 548 97 145 24 1162
Fragments arranged by size:
1162 548 181 145 97 24
BsiHKAI G_wGCw'C
Cuts at: 0 488 2157
Size: 488 1669
Bsp1286I G_dGCh'C
Cuts at: 0 488 1517 2157
Size: 488 1029 640
Fragments arranged by size:
1029 640 488
BspEI T'CCGG_A
Cuts at: 0 1775 2157
Size: 1775 382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 993 2157
Size: 993 1164
BsrBI CCG'CTC
Cuts at: 0 1281 2157
Size: 1281 876
BsrDI GCAATG_nn'
Cuts at: 0 728 931 2157
Size: 728 203 1226
Fragments arranged by size:
1226 728 203
BstYI r'GATC_y
Cuts at: 0 356 473 493 876 1163 2081 2157
Size: 356 117 20 383 287 918 76
Fragments arranged by size:
918 383 356 287 117 76 20
BtsI GCAGTG_nn'
Cuts at: 0 1760 2157
Size: 1760 397
DraIII CAC_nnn'GTG
Cuts at: 0 335 2157
Size: 335 1822
EaeI y'GGCC_r
Cuts at: 0 170 428 2157
Size: 170 258 1729
Fragments arranged by size:
1729 258 170
EarI CTCTTCn'nnn_
Cuts at: 0 241 265 1024 2157
Size: 241 24 759 1133
Fragments arranged by size:
1133 759 241 24
EcoICRI GAG'CTC
Cuts at: 0 486 2157
Size: 486 1671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 225 334 535 694 755 775 1051
Size: 91 134 109 201 159 61 20 276
Cuts at: 1051 1228 1690 1807 2157
Size: 177 462 117 350
Fragments arranged by size:
462 350 276 201 177 159 134 117
109 91 61 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 1326 2157
Size: 160 1166 831
Fragments arranged by size:
1166 831 160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1719 2157
Size: 1719 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 2066 2098 2157
Size: 637 32 1397 32 59
Fragments arranged by size:
1397 637 59 32 32
HincII GTy'rAC
Cuts at: 0 801 885 956 1110 2157
Size: 801 84 71 154 1047
Fragments arranged by size:
1047 801 154 84 71
HpaI GTT'AAC
Cuts at: 0 1110 2157
Size: 1110 1047
KpnI G_GTAC'C
Cuts at: 0 227 2157
Size: 227 1930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1170 2157
Size: 1170 987
MscI TGG'CCA
Cuts at: 0 172 2157
Size: 172 1985
MslI CAynn'nnrTG
Cuts at: 0 388 2157
Size: 388 1769
MspA1I CmG'CkG
Cuts at: 0 21 1314 1473 2157
Size: 21 1293 159 684
Fragments arranged by size:
1293 684 159 21
PasI CC'CwG_GG
Cuts at: 0 1385 2157
Size: 1385 772
PflMI CCAn_nnn'nTGG
Cuts at: 0 470 1041 2157
Size: 470 571 1116
Fragments arranged by size:
1116 571 470
PfoI T'CCnGG_A
Cuts at: 0 59 260 576 1784 2157
Size: 59 201 316 1208 373
Fragments arranged by size:
1208 373 316 201 59
PshAI GACnn'nnGTC
Cuts at: 0 572 2157
Size: 572 1585
PstI C_TGCA'G
Cuts at: 0 137 1583 1767 2157
Size: 137 1446 184 390
Fragments arranged by size:
1446 390 184 137
PvuII CAG'CTG
Cuts at: 0 21 1314 1473 2157
Size: 21 1293 159 684
Fragments arranged by size:
1293 684 159 21
SacI G_AGCT'C
Cuts at: 0 488 2157
Size: 488 1669
SbfI CC_TGCA'GG
Cuts at: 0 137 2157
Size: 137 2020
SexAI A'CCwGG_T
Cuts at: 0 949 2157
Size: 949 1208
SfcI C'TryA_G
Cuts at: 0 133 1579 1763 2157
Size: 133 1446 184 394
Fragments arranged by size:
1446 394 184 133
SmaI CCC'GGG
Cuts at: 0 1547 2157
Size: 1547 610
SmlI C'TyrA_G
Cuts at: 0 204 2157
Size: 204 1953
StuI AGG'CCT
Cuts at: 0 1896 2157
Size: 1896 261
StyI C'CwwG_G
Cuts at: 0 497 1703 2035 2157
Size: 497 1206 332 122
Fragments arranged by size:
1206 497 332 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1506 1924 2157
Size: 1506 418 233
Fragments arranged by size:
1506 418 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 766 798 1936 1968 2157
Size: 691 32 43 32 1138 32 189
Fragments arranged by size:
1138 691 189 43 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1929 2033 2157
Size: 1929 104 124
Fragments arranged by size:
1929 124 104
XmaI C'CCGG_G
Cuts at: 0 1545 2157
Size: 1545 612
ZraI GAC'GTC
Cuts at: 0 129 2157
Size: 129 2028
Enzymes that do cut:
AatII Acc65I AcuI AflII AlwNI AvaI BaeI BamHI
BanI BanII BbsI BcgI BclI BlpI BmgBI BmrI
BplI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BstYI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
FalI Hin4I HincII HpaI KpnI MmeI MscI MslI
MspA1I PasI PflMI PfoI PshAI PstI PvuII SacI
SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BbvCI BciVI BfrBI BglI BglII Bme1580I
BmtI Bpu10I BpuEI BsaAI BsiEI BsiWI BsmI BsmBI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HindIII KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI