(Circular) (Six-base) MAPSORT of: pFN21AE2329.seq Check: 8273 from: 1 to: 8644 pFN21AE2329 8644 bp With 182 enzymes: SgfI * February 23, 2011 17:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3122 5632 3122 Size: 2510 6134 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6454 278 Size: 53 83 186 936 4918 2468 Fragments arranged by size: 4918 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 3242 3269 5714 5987 1937 Size: 831 474 27 2445 273 4594 Fragments arranged by size: 4594 2445 831 474 273 27 Acc65I G'GTAC_C Cuts at: 5965 5965 Size: 8644 AclI AA'CG_TT Cuts at: 1563 6771 7144 1563 Size: 5208 373 3063 Fragments arranged by size: 5208 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 3585 3837 4176 5259 5436 5898 6707 Size: 881 1072 252 339 1083 177 462 809 Cuts at: 6707 8091 8359 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1083 1072 881 809 462 339 268 252 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8447 AflIII A'CryG_T Cuts at: 1203 7543 8520 1203 Size: 6340 977 1327 Fragments arranged by size: 6340 1327 977 AgeI A'CCGG_T Cuts at: 7475 7475 Size: 8644 AhdI GACnn_n'nnGTC Cuts at: 4640 7373 4640 Size: 2733 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5136 5325 1184 Size: 3952 189 4503 Fragments arranged by size: 4503 3952 189 AlwNI CAG_nnn'CTG Cuts at: 3824 4557 6387 7959 3824 Size: 733 1830 1572 4509 Fragments arranged by size: 4509 1830 1572 733 ApaI G_GGCC'C Cuts at: 2478 5078 2478 Size: 2600 6044 ApaLI G'TGCA_C Cuts at: 5856 6701 7857 5856 Size: 845 1156 6643 Fragments arranged by size: 6643 1156 845 ApoI r'AATT_y Cuts at: 1493 3302 3433 3538 3616 3947 4400 5953 6179 Size: 1809 131 105 78 331 453 1553 226 Cuts at: 6179 6331 8440 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1809 1697 1553 453 331 226 152 131 105 78 AscI GG'CGCG_CC Cuts at: 2426 2426 Size: 8644 AseI AT'TA_AT Cuts at: 160 1032 7198 160 Size: 872 6166 1606 Fragments arranged by size: 6166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8644 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2143 2344 3372 3732 4009 5969 Size: 73 138 62 201 1028 360 277 1960 Cuts at: 5969 1870 Size: 4545 Fragments arranged by size: 4545 1960 1028 360 277 201 138 73 62 BamHI G'GATC_C Cuts at: 3193 5974 6341 3193 Size: 2781 367 5496 Fragments arranged by size: 5496 2781 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2348 2387 5236 5965 7420 Size: 325 217 627 561 39 2849 729 1455 Cuts at: 7420 618 Size: 1842 Fragments arranged by size: 2849 1842 1455 729 627 561 325 217 39 BanII G_rGCy'C Cuts at: 1391 2210 2478 3123 3572 4931 5078 5296 5387 Size: 819 268 645 449 1359 147 218 91 Cuts at: 5387 5963 1391 Size: 576 4072 Fragments arranged by size: 4072 1359 819 645 576 449 268 218 147 91 BbeI G_GCGC'C Cuts at: 2352 2391 2352 Size: 39 8605 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3843 928 Size: 971 1944 5729 Fragments arranged by size: 5729 1944 971 BbvCI CC'TCA_GC Cuts at: 5133 5445 5133 Size: 312 8332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6835 6869 1903 Size: 34 50 34 4814 34 3678 Fragments arranged by size: 4814 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2163 3964 4923 6536 7746 2163 Size: 1801 959 1613 1210 3061 Fragments arranged by size: 3061 1801 1613 1210 959 BclI T'GATC_A Cuts at: 1548 4497 1548 Size: 2949 5695 BfrBI ATG'CAT Cuts at: 5321 5321 Size: 8644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2086 2110 2357 Size: 107 122 71 631 605 414 24 247 Cuts at: 2357 3763 5543 7255 136 Size: 1406 1780 1712 1525 Fragments arranged by size: 1780 1712 1525 1406 631 605 414 247 122 107 71 24 BglII A'GATC_T Cuts at: 8639 8639 Size: 8644 BlpI GC'TnA_GC Cuts at: 2011 4722 5394 6061 2011 Size: 2711 672 667 4594 Fragments arranged by size: 4594 2711 672 667 Bme1580I G_kGCm'C Cuts at: 1790 2478 3279 3361 3456 5078 5860 6705 7861 Size: 688 801 82 95 1622 782 845 1156 Cuts at: 7861 1790 Size: 2573 Fragments arranged by size: 2573 1622 1156 845 801 782 688 95 82 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3355 3963 4104 Size: 416 53 83 399 24 1938 608 141 Cuts at: 4104 4916 5502 6375 7333 442 Size: 812 586 873 958 1753 Fragments arranged by size: 1938 1753 958 873 812 608 586 416 399 141 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2229 2229 Size: 8644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 3022 3137 3745 3801 3972 4854 7304 Size: 842 941 115 608 56 171 882 2450 Cuts at: 7304 1239 Size: 2579 Fragments arranged by size: 2579 2450 941 882 842 608 171 115 56 Bpu10I CC'TnA_GC Cuts at: 4524 5133 5445 4524 Size: 609 312 7723 Fragments arranged by size: 7723 609 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3179 4809 6769 7634 7932 3179 Size: 1630 1960 865 298 3891 Fragments arranged by size: 3891 1960 1630 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2297 2665 4083 7307 882 Size: 617 798 368 1418 3224 2219 Fragments arranged by size: 3224 2219 1418 798 617 368 BsaAI yAC'GTr Cuts at: 493 1204 3246 5740 6435 493 Size: 711 2042 2494 695 2702 Fragments arranged by size: 2702 2494 2042 711 695 BsaBI GATnn'nnATC Cuts at: 8638 8638 Size: 8644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 2388 2415 4964 Size: 53 83 186 936 816 39 27 2549 Cuts at: 4964 6451 6833 7468 275 Size: 1487 382 635 1451 Fragments arranged by size: 2549 1487 1451 936 816 635 382 186 83 53 39 27 BsaWI w'CCGG_w Cuts at: 7077 7475 7749 7896 8307 7077 Size: 398 274 147 411 7414 Fragments arranged by size: 7414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8419 Fragments arranged by size: 8419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2016 2653 2677 3975 1186 Size: 830 637 24 1298 5855 Fragments arranged by size: 5855 1298 830 637 24 BseYI C'CCAG_C Cuts at: 2499 3885 3917 4589 4672 5059 5253 5659 7847 Size: 1386 32 672 83 387 194 406 2188 Cuts at: 7847 8343 2499 Size: 496 2800 Fragments arranged by size: 2800 2188 1386 672 496 406 387 194 83 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5408 5487 1163 Size: 573 3672 79 4320 Fragments arranged by size: 4320 3672 573 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2105 2361 3755 6094 6855 Size: 275 186 18 86 256 1394 2339 761 Cuts at: 6855 7883 1540 Size: 1028 2301 Fragments arranged by size: 2339 2301 1394 1028 761 275 256 186 86 18 BsiHKAI G_wGCw'C Cuts at: 2210 4626 5860 6705 6790 7861 2210 Size: 2416 1234 845 85 1071 2993 Fragments arranged by size: 2993 2416 1234 1071 845 85 BsmI GAATG_Cn' Cuts at: 6155 6248 6155 Size: 93 8551 BsmBI CGTCTCn'nnnn_ Cuts at: 2422 3798 2422 Size: 1376 7268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2478 3123 3279 3361 3456 3572 Size: 399 420 268 645 156 82 95 116 Cuts at: 3572 4626 4931 5078 5296 5387 5860 5963 6705 Size: 1054 305 147 218 91 473 103 742 Cuts at: 6705 6790 7861 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1054 742 645 473 420 399 305 268 218 156 147 116 103 95 91 85 82 BspEI T'CCGG_A Cuts at: 8307 8307 Size: 8644 BspHI T'CATG_A Cuts at: 5296 6533 8263 5296 Size: 1237 1730 5677 Fragments arranged by size: 5677 1730 1237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3122 5632 5999 844 Size: 2278 2510 367 3489 Fragments arranged by size: 3489 2510 2278 367 BsrBI CCG'CTC Cuts at: 1394 1823 2118 2383 6531 1394 Size: 429 295 265 4148 3507 Fragments arranged by size: 4148 3507 429 295 265 BsrDI GCAATG_nn' Cuts at: 65 1248 7139 7313 65 Size: 1183 5891 174 1396 Fragments arranged by size: 5891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 2464 5707 7288 7475 1700 Size: 110 294 360 3243 1581 187 2869 Fragments arranged by size: 3243 2869 1581 360 294 187 110 BsrGI T'GTAC_A Cuts at: 96 5755 96 Size: 5659 2985 BssHII G'CGCG_C Cuts at: 1925 2426 1925 Size: 501 8143 BssSI C'ACGA_G Cuts at: 6704 7716 6704 Size: 1012 7632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8644 BstBI TT'CG_AA Cuts at: 7448 7448 Size: 8644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8644 BstXI CCAn_nnnn'nTGG Cuts at: 5489 5489 Size: 8644 BstYI r'GATC_y Cuts at: 1972 3193 3584 5974 6341 6726 6743 8195 8281 Size: 1221 391 2390 367 385 17 1452 86 Cuts at: 8281 8293 8639 1972 Size: 12 346 1977 Fragments arranged by size: 2390 1977 1452 1221 391 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3270 5715 3270 Size: 2445 6199 Bsu36I CC'TnA_GG Cuts at: 4638 5745 4638 Size: 1107 7537 BtgI C'CryG_G Cuts at: 513 1065 2037 2577 2676 5379 513 Size: 552 972 540 99 2703 3778 Fragments arranged by size: 3778 2703 972 552 540 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3648 5327 487 Size: 683 56 2422 1679 3804 Fragments arranged by size: 3804 2422 1679 683 56 BtsI GCAGTG_nn' Cuts at: 6160 6954 6974 6160 Size: 794 20 7830 Fragments arranged by size: 7830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8609 DraI TTT'AAA Cuts at: 2887 3025 3309 4395 5948 2887 Size: 138 284 1086 1553 5583 Fragments arranged by size: 5583 1553 1086 284 138 DraIII CAC_nnn'GTG Cuts at: 3971 3971 Size: 8644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7651 809 Size: 507 6335 1802 Fragments arranged by size: 6335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2043 2102 Size: 54 1425 50 275 18 186 27 59 Cuts at: 2102 2358 2673 3659 3752 3776 5556 6091 6980 Size: 256 315 986 93 24 1780 535 889 Cuts at: 6980 8609 8 Size: 1629 43 Fragments arranged by size: 1780 1629 1425 986 889 535 315 275 256 186 93 59 54 50 43 27 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 2102 2358 3752 6091 1537 Size: 275 204 86 256 1394 2339 4090 Fragments arranged by size: 4090 2339 1394 275 256 204 86 EarI CTCTTCn'nnn_ Cuts at: 1353 3729 4698 6574 1353 Size: 2376 969 1876 3423 Fragments arranged by size: 3423 2376 1876 969 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2128 2149 2209 7231 7605 7751 Size: 1064 138 539 21 60 5022 374 146 Cuts at: 7751 387 Size: 1280 Fragments arranged by size: 5022 1280 1064 539 374 146 138 60 21 EcoICRI GAG'CTC Cuts at: 2208 2208 Size: 8644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2513 3022 3137 3585 3745 3801 Size: 393 449 432 509 115 448 160 56 Cuts at: 3801 3837 3972 4176 4854 5259 5436 5898 6707 Size: 36 135 204 678 405 177 462 809 Cuts at: 6707 7304 8091 8359 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 809 787 678 597 509 462 449 448 432 405 393 268 204 177 160 135 115 56 36 EcoO109I rG'GnC_Cy Cuts at: 2475 3112 3713 3765 3860 4634 2475 Size: 637 601 52 95 774 6485 Fragments arranged by size: 6485 774 637 601 95 52 EcoRI G'AATT_C Cuts at: 3538 3616 5953 6331 3538 Size: 78 2337 378 5851 Fragments arranged by size: 5851 2337 378 78 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3075 3606 4452 5376 3075 Size: 531 846 924 6343 Fragments arranged by size: 6343 924 846 531 FspI TGC'GCA Cuts at: 5406 7150 5406 Size: 1744 6900 FspAI rTGC'GCAy Cuts at: 5406 5406 Size: 8644 HaeII r_GCGC'y Cuts at: 1678 2092 2352 2391 7791 1678 Size: 414 260 39 5400 2531 Fragments arranged by size: 5400 2531 414 260 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3020 3052 4482 4514 4626 Size: 32 135 32 1448 32 1430 32 112 Cuts at: 4626 4658 7285 7317 7359 7391 1373 Size: 32 2627 32 42 32 2626 Fragments arranged by size: 2627 2626 1448 1430 135 112 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5988 6234 669 Size: 988 281 4050 246 3079 Fragments arranged by size: 4050 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8644 HpaI GTT'AAC Cuts at: 6234 6234 Size: 8644 KasI G'GCGC_C Cuts at: 2348 2387 2348 Size: 39 8605 KpnI G_GTAC'C Cuts at: 5969 5969 Size: 8644 MfeI C'AATT_G Cuts at: 6243 6243 Size: 8644 MluI A'CGCG_T Cuts at: 8520 8520 Size: 8644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4175 5655 5807 7758 7942 1305 Size: 2870 1480 152 1951 184 2007 Fragments arranged by size: 2870 2007 1951 1480 184 152 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 3661 5558 10 Size: 54 1425 343 213 1616 1897 3096 Fragments arranged by size: 3096 1897 1616 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 4973 5043 5193 6602 6961 7120 518 Size: 648 3807 70 150 1409 359 159 2042 Fragments arranged by size: 3807 2042 1409 648 359 159 150 70 MspA1I CmG'CkG Cuts at: 1606 2015 2039 2101 2579 2610 4181 4721 5023 Size: 409 24 62 478 31 1571 540 302 Cuts at: 5023 5072 6060 6737 7885 8130 1606 Size: 49 988 677 1148 245 2120 Fragments arranged by size: 2120 1571 1148 988 677 540 478 409 302 245 62 49 31 24 NaeI GCC'GGC Cuts at: 2106 5709 2106 Size: 3603 5041 NarI GG'CG_CC Cuts at: 2349 2388 2349 Size: 39 8605 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8092 NdeI CA'TA_TG Cuts at: 387 387 Size: 8644 NgoMIV G'CCGG_C Cuts at: 2104 5707 2104 Size: 3603 5041 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8644 NotI GC'GGCC_GC Cuts at: 2016 6091 2016 Size: 4075 4569 NsiI A_TGCA'T Cuts at: 5323 5323 Size: 8644 NspI r_CATG'y Cuts at: 1132 1229 3850 4680 5168 5312 5325 6002 7547 Size: 97 2621 830 488 144 13 677 1545 Cuts at: 7547 1132 Size: 2229 Fragments arranged by size: 2621 2229 1545 830 677 488 144 97 13 PasI CC'CwG_GG Cuts at: 1348 3123 1348 Size: 1775 6869 PciI A'CATG_T Cuts at: 7543 7543 Size: 8644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8644 PfoI T'CCnGG_A Cuts at: 3490 3490 Size: 8644 PmeI GTTT'AAAC Cuts at: 5948 5948 Size: 8644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6713 6745 8226 8258 6713 Size: 32 1481 32 7099 Fragments arranged by size: 7099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3112 3713 4634 3112 Size: 601 921 7122 Fragments arranged by size: 7122 921 601 PshAI GACnn'nnGTC Cuts at: 1613 3790 4075 1613 Size: 2177 285 6182 Fragments arranged by size: 6182 2177 285 PsiI TTA'TAA Cuts at: 6214 6214 Size: 8644 PspOMI G'GGCC_C Cuts at: 2474 5074 2474 Size: 2600 6044 PspXI vC'TCGA_Gb Cuts at: 3372 3372 Size: 8644 PstI C_TGCA'G Cuts at: 830 4285 5996 830 Size: 3455 1711 3478 Fragments arranged by size: 3478 3455 1711 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8644 PvuII CAG'CTG Cuts at: 4181 4721 4181 Size: 540 8104 SacI G_AGCT'C Cuts at: 2210 2210 Size: 8644 SacII CC_GC'GG Cuts at: 2040 2580 2040 Size: 540 8104 SalI G'TCGA_C Cuts at: 1936 5986 1936 Size: 4050 4594 SbfI CC_TGCA'GG Cuts at: 4285 5996 4285 Size: 1711 6933 ScaI AGT'ACT Cuts at: 6892 8604 6892 Size: 1712 6932 SfcI C'TryA_G Cuts at: 826 1045 4281 5125 5200 5861 5992 7127 7808 Size: 219 3236 844 75 661 131 1135 681 Cuts at: 7808 7999 826 Size: 191 1471 Fragments arranged by size: 3236 1471 1135 844 681 661 219 191 131 75 SfoI GGC'GCC Cuts at: 2350 2389 2350 Size: 39 8605 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8644 SmaI CCC'GGG Cuts at: 1872 2083 2145 2346 5971 1872 Size: 211 62 201 3625 4545 Fragments arranged by size: 4545 3625 211 201 62 SmlI C'TyrA_G Cuts at: 820 1017 1943 3158 3372 4824 6748 7649 7911 Size: 197 926 1215 214 1452 1924 901 262 Cuts at: 7911 820 Size: 1553 Fragments arranged by size: 1924 1553 1452 1215 926 901 262 214 197 SnaBI TAC'GTA Cuts at: 493 3246 6435 493 Size: 2753 3189 2702 Fragments arranged by size: 3189 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8644 SphI G_CATG'C Cuts at: 1132 5312 6002 1132 Size: 4180 690 3774 Fragments arranged by size: 4180 3774 690 SrfI GCCC'GGGC Cuts at: 2346 2346 Size: 8644 SspI AAT'ATT Cuts at: 5 52 4300 6568 5 Size: 47 4248 2268 2081 Fragments arranged by size: 4248 2268 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8644 StyI C'CwwG_G Cuts at: 513 1065 2287 2421 3866 4651 6083 513 Size: 552 1222 134 1445 785 1432 3074 Fragments arranged by size: 3074 1445 1432 1222 785 552 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3982 5073 5442 5521 6671 6688 Size: 1255 1067 1335 1091 369 79 1150 17 Cuts at: 6688 6841 7026 7471 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1335 1255 1150 1091 1067 445 369 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2848 2939 3363 Size: 274 80 33 51 1443 871 91 424 Cuts at: 3363 5755 5917 6890 8602 96 Size: 2392 162 973 1712 138 Fragments arranged by size: 2392 1712 1443 973 871 424 274 162 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8604 8636 8604 Size: 32 8612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8644 XbaI T'CTAG_A Cuts at: 5980 5980 Size: 8644 XhoI C'TCGA_G Cuts at: 1943 3372 1943 Size: 1429 7215 XmaI C'CCGG_G Cuts at: 1870 2081 2143 2344 5969 1870 Size: 211 62 201 3625 4545 Fragments arranged by size: 4545 3625 211 201 62 XmnI GAAnn'nnTTC Cuts at: 5654 6773 5654 Size: 1119 7525 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6452 276 Size: 53 83 186 936 4918 2468 Fragments arranged by size: 4918 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BsiWI EcoNI EcoRV FseI NruI PacI PmlI PsrI RsrII SanDI SapI SexAI SfiI SwaI XcmI