Restriction map for
pFN21AE1755
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AvaI AvaII AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
TaqI Tru9I TthHB8I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 701 GGTACC G^GTACC
AcsI 764 AAATTT [GA]^AATT[CT]
AfaI 702 GTAC GT^AC
ApoI 764 AAATTT [GA]^AATT[CT]
Asp718I 701 GGTACC G^GTACC
AspI 580 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 287 TGGCCA TGG^CCA
BamHI 401 GGATCC G^GATCC
BbvI 645 GCTGC GCAGC(8/12)
BcnI 423 CCGGG CC^[GC]GG
BsmBI 432 CGTCTC CGTCTC(1/5)
BsrDI 549 CATTGC GCAATG(2/0)
BstAPI 315 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 420 CATCC GGATG(2/0)
BstYI 401 GGATCC [GA]^GATC[CT]
CfoI 417 GCGC GCG^C
DraIII 316 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 287 TGGCCA [CT]^GGCC[GA]
EcoNI 620 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 420 CATCC GGATG(9/13)
HgaI 349 GACGC GACGC(5/10)
HhaI 417 GCGC GCG^C
HinP1I 417 GCGC G^CGC
KpnI 701 GGTACC GGTAC^C
MflI 401 GGATCC [GA]^GATC[CT]
MluNI 287 TGGCCA TGG^CCA
MscI 287 TGGCCA TGG^CCA
MslI 511 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 423 CCGGG CC^[GC]GG
PflFI 580 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 293 GAGTC GAGTC(4/5)
PvuII 80 CAGCTG CAG^CTG
RsaI 702 GTAC GT^AC
SapI 73 GAAGAGC GCTCTTC(1/4)
SfaNI 419 GCATC GCATC(5/9)
SmlI 26 CTCAAG C^T[CT][GA]AG
SspI 269 AATATT AAT^ATT
StuI 375 AGGCCT AGG^CCT
TseI 645 GCTGC G^C[AT]GC
Tsp509I 765 AATT ^AATT
Tth111I 580 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 411 TCTAGA T^CTAGA
XhoII 401 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BanI 568 GGTGCC G^G[CT][GA]CC
701 GGTACC
BanII 23 GGGCTC G[GA]GC[CT]^C
543 GAGCCC
BbsI 586 GTCTTC GAAGAC(2/6)
710 GTCTTC
BbvCI 184 GCTGAGG CCTCAGC(-5/-2)
226 GCTGAGG
BfaI 164 CTAG C^TAG
412 CTAG
BpmI 621 CTCCAG CTGGAG(16/14)
68 CTGGAG
BpuAI 586 GTCTTC GAAGAC(2/6)
710 GTCTTC
BseRI 52 GAGGAG GAGGAG(10/8)
92 GAGGAG
BstXI 391 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCAGGAGGGTGG
DpnII 402 GATC ^GATC
427 GATC
EarI 73 GAAGAG CTCTTC(1/4)
114 GAAGAG
HapII 423 CCGG C^CGG
591 CCGG
HpaII 423 CCGG C^CGG
591 CCGG
Ksp632I 73 GAAGAG CTCTTC(1/4)
114 GAAGAG
MaeI 164 CTAG C^TAG
412 CTAG
MaeII 340 ACGT A^CGT
431 ACGT
MboI 402 GATC ^GATC
427 GATC
MspA1I 80 CAGCTG C[AC]G^C[GT]G
649 CCGCTG
MspI 423 CCGG C^CGG
591 CCGG
NdeII 402 GATC ^GATC
427 GATC
PflMI 136 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
246 CCAAGAGTTGG
PstI 198 CTGCAG CTGCA^G
573 CTGCAG
Sau3AI 402 GATC ^GATC
427 GATC
SfcI 198 CTGCAG C^T[GA][CT]AG
573 CTGCAG
Tsp45I 442 GTGAC ^GT[GC]AC
494 GTGAC
Van91I 136 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
246 CCAAGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI AspHI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIV Sau96I ScrFI TfiI
TspRI