################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccII AcyI AflII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 426 GGTACC G^GTACC AccIII 342 TCCGGA T^CCGGA AciI 24 GCGG CCGC(-3/-1) AclI 316 AACGTT AA^CGTT AflIII 181 ACATGT A^C[GA][CT]GT AlwNI 392 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 5 AGCGCT AGC^GCT Asp718I 426 GGTACC G^GTACC AvaII 789 GGTCC G^G[AT]CC BalI 817 TGGCCA TGG^CCA BanI 426 GGTACC G^G[CT][GA]CC BbvI 21 GCAGC GCAGC(8/12) BciVI 353 GGATAC GTATCC(6/5) BfaI 232 CTAG C^TAG BglII 561 AGATCT A^GATCT BsaJI 497 CCCTGG C^C[ACGT][ACGT]GG BsaWI 342 TCCGGA [AT]^CCGG[AT] BseAI 342 TCCGGA T^CCGGA BsmAI 367 GAGAC GTCTC(1/5) BsmI 462 GCATTC GAATGC(1/-1) BspEI 342 TCCGGA T^CCGGA BspLU11I 181 ACATGT A^CATGT BsrDI 763 CATTGC GCAATG(2/0) BssSI 709 CTCGTG CACGAG(-5/-1) BstAPI 130 GCATTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 561 AGATCT [GA]^GATC[CT] CfoI 6 GCGC GCG^C Cfr13I 789 GGTCC G^G[ACGT]CC DdeI 567 CTTAG C^T[ACGT]AG EaeI 817 TGGCCA [CT]^GGCC[GA] Eco47III 5 AGCGCT AGC^GCT EcoNI 71 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 840 GAATTC G^AATTC Fnu4HI 21 GCAGC GC^[ACGT]GC HaeII 5 AGCGCT [GA]GCGC^[CT] HaeIII 818 GGCC GG^CC HapII 343 CCGG C^CGG HhaI 6 GCGC GCG^C HinP1I 6 GCGC G^CGC HpaII 343 CCGG C^CGG HphI 54 GGTGA GGTGA(8/7) ItaI 21 GCAGC GC^[ACGT]GC KpnI 426 GGTACC GGTAC^C MaeI 232 CTAG C^TAG MaeII 317 ACGT A^CGT MflI 561 AGATCT [GA]^GATC[CT] MluNI 817 TGGCCA TGG^CCA MroI 342 TCCGGA T^CCGGA MscI 817 TGGCCA TGG^CCA MspA1I 22 CAGCGG C[AC]G^C[GT]G MspI 343 CCGG C^CGG NdeI 614 CATATG CA^TATG NspI 181 ACATGT [GA]CATG^[CT] Psp1406I 316 AACGTT AA^CGTT Sau96I 789 GGTCC G^G[ACGT]CC SfcI 470 CTGTAG C^T[GA][CT]AG SspI 963 AATATT AAT^ATT TaqI 228 TCGA T^CGA TfiI 523 GATTC G^A[AT]TC TseI 21 GCAGC G^C[AT]GC TthHB8I 228 TCGA T^CGA XhoII 561 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 427 GTAC GT^AC 502 GTAC AluI 255 AGCT AG^CT 339 AGCT BanII 12 GGGCTC G[GA]GC[CT]^C 224 GGGCTC BmyI 12 GGGCTC G[AGT]GC[ACT]^C 224 GGGCTC BseRI 61 GAGGAG GAGGAG(10/8) 322 GAGGAG BsgI 19 GTGCAG GTGCAG(16/14) 545 GTGCAG BsiYI 17 CCGTGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 71 CCTCCAAAAGG BslI 17 CCGTGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 71 CCTCCAAAAGG Bsp1286I 12 GGGCTC G[AGT]GC[ACT]^C 224 GGGCTC BstXI 214 CCATACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 798 CCATCATCTTGG Cac8I 458 GCTGGC GC[ACGT]^[ACGT]GC 815 GCTGGC MboII 724 GAAGA GAAGA(8/7) 648 TCTTC MslI 514 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 763 CATTGCAGTG PleI 883 GACTC GAGTC(4/5) 950 GACTC RsaI 427 GTAC GT^AC 502 GTAC SfaNI 136 GATGC GCATC(5/9) 84 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AcsI ApoI BsrI BssKI BstF5I BstNI DpnII DraI EcoRII FokI HinfI MaeIII MboI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI Tru9I Tsp45I Tsp509I TspRI