Restriction map for
pFN21AE0188
(Circular) (Six-base) MAPSORT of: pFN21AE0188.seq Check: 6309 from: 1 to: 5680
pFN21AE0188 5680 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 2431 3001 2431
Size: 570 5110
AclI AA'CG_TT
Cuts at: 1563 2322 3807 4180 1563
Size: 759 1485 373 3063
Fragments arranged by size:
3063 1485 759 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AfeI AGC'GCT
Cuts at: 2012 2012
Size: 5680
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2186 4579 5556 1203
Size: 983 2393 977 1327
Fragments arranged by size:
2393 1327 983 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2402 3423 4995 2402
Size: 1021 1572 3087
Fragments arranged by size:
3087 1572 1021
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2278 2309 2489 2845 2945 2989 3215 3367
Size: 785 31 180 356 100 44 226 152
Cuts at: 3367 5476 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 785 356 226 180 152 100
44 31
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 3001 4456 618
Size: 325 217 627 644 570 1455 1842
Fragments arranged by size:
1842 1455 644 627 570 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2233 2999 1391
Size: 630 212 766 4072
Fragments arranged by size:
4072 766 630 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3572 4782 2352
Size: 1220 1210 3250
Fragments arranged by size:
3250 1220 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2566 5675 2566
Size: 3109 2571
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4897 1790
Size: 1951 1156 2573
Fragments arranged by size:
2573 1951 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2390 3411 4369
Size: 416 53 83 399 24 973 1021 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1021 973 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2347 4113 4511 4785 4932 5343 2347
Size: 1766 398 274 147 411 2684
Fragments arranged by size:
2684 1766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2342 1186
Size: 895 261 4524
Fragments arranged by size:
4524 895 261
BseYI C'CCAG_C
Cuts at: 4883 5379 4883
Size: 496 5184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2571 1163
Size: 573 309 526 4272
Fragments arranged by size:
4272 573 526 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 2467 3191 3284 2467
Size: 724 93 4863
Fragments arranged by size:
4863 724 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2233 2999 3741 3826 4897 1391
Size: 399 231 212 766 742 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 399 231 212 85
BspEI T'CCGG_A
Cuts at: 2347 5343 2347
Size: 2996 2684
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2767 4175 4349 65
Size: 1183 1519 1408 174 1396
Fragments arranged by size:
1519 1408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 2714 3740 4752 2714
Size: 1026 1012 3642
Fragments arranged by size:
3642 1026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2141 794
Size: 1347 4333
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2226 2810 2226
Size: 584 5096
BstYI r'GATC_y
Cuts at: 1972 2566 3010 3377 3762 3779 5231 5317 5329
Size: 594 444 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 594 444 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2783 487
Size: 683 56 1557 3384
Fragments arranged by size:
3384 1557 683 56
BtsI GCAGTG_nn'
Cuts at: 2559 2779 3196 3990 4010 2559
Size: 220 417 794 20 4229
Fragments arranged by size:
4229 794 417 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2101 2275 2928 2984 2101
Size: 174 653 56 4797
Fragments arranged by size:
4797 653 174 56
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2822 3127 4016
Size: 54 1425 50 275 18 992 305 889
Cuts at: 4016 5645 8
Size: 1629 43
Fragments arranged by size:
1629 1425 992 889 305 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2080 2080
Size: 5680
EcoRI G'AATT_C
Cuts at: 2845 2989 3367 2845
Size: 144 378 5158
Fragments arranged by size:
5158 378 144
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2014 4827 1678
Size: 336 2813 2531
Fragments arranged by size:
2813 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2702 2734 4321 4353 4395
Size: 32 135 32 1130 32 1587 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1130 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 2435 3005 2435
Size: 570 5110
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2189 4794 4978 1305
Size: 884 2605 184 2007
Fragments arranged by size:
2605 2007 884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2824 10
Size: 54 1425 343 992 2866
Fragments arranged by size:
2866 1425 992 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2772 3638 3997 4156 518
Size: 648 1357 249 866 359 159 2042
Fragments arranged by size:
2042 1357 866 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3096 3773 4921 5166 1606
Size: 423 1067 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5128
NdeI CA'TA_TG
Cuts at: 387 2620 387
Size: 2233 3447
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2190 3038 4583 1132
Size: 97 961 848 1545 2229
Fragments arranged by size:
2229 1545 961 848 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2186 4579 2186
Size: 2393 3287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2475 3028 4163 4844 5035 826
Size: 219 1430 553 1135 681 191 1471
Fragments arranged by size:
1471 1430 1135 681 553 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 2970 3604 5
Size: 47 2918 634 2081
Fragments arranged by size:
2918 2081 634 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 3119 513
Size: 552 2054 3074
Fragments arranged by size:
3074 2054 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3707 3724 3877 4062 4507 325
Size: 1255 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI