Restriction map for
pFN21AE0188
(Linear) (Six-base) MAPSORT of: KIEE0188.seq Check: 3005 from: 1 to: 975
KIEE0188.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
Acc65I G'GTAC_C
Cuts at: 0 426 975
Size: 426 549
AclI AA'CG_TT
Cuts at: 0 317 975
Size: 317 658
AfeI AGC'GCT
Cuts at: 0 7 975
Size: 7 968
AflIII A'CryG_T
Cuts at: 0 181 975
Size: 181 794
AlwNI CAG_nnn'CTG
Cuts at: 0 397 975
Size: 397 578
ApoI r'AATT_y
Cuts at: 0 273 304 484 840 940 975
Size: 273 31 180 356 100 35
Fragments arranged by size:
356 273 180 100 35 31
BanI G'GyrC_C
Cuts at: 0 426 975
Size: 426 549
BanII G_rGCy'C
Cuts at: 0 16 228 975
Size: 16 212 747
Fragments arranged by size:
747 212 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 347 975
Size: 347 628
BglII A'GATC_T
Cuts at: 0 561 975
Size: 561 414
BmrI ACTGGGnnnn_n'
Cuts at: 0 385 975
Size: 385 590
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 975
Size: 87 888
BsaWI w'CCGG_w
Cuts at: 0 342 975
Size: 342 633
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 337 975
Size: 76 261 638
Fragments arranged by size:
638 261 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 566 975
Size: 40 526 409
Fragments arranged by size:
526 409 40
BsmI GAATG_Cn'
Cuts at: 0 462 975
Size: 462 513
Bsp1286I G_dGCh'C
Cuts at: 0 16 228 975
Size: 16 212 747
Fragments arranged by size:
747 212 16
BspEI T'CCGG_A
Cuts at: 0 342 975
Size: 342 633
BsrDI GCAATG_nn'
Cuts at: 0 762 975
Size: 762 213
BssSI C'ACGA_G
Cuts at: 0 709 975
Size: 709 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 136 975
Size: 136 839
BstXI CCAn_nnnn'nTGG
Cuts at: 0 221 805 975
Size: 221 584 170
Fragments arranged by size:
584 221 170
BstYI r'GATC_y
Cuts at: 0 561 975
Size: 561 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 778 975
Size: 778 197
BtsI GCAGTG_nn'
Cuts at: 0 554 774 975
Size: 554 220 201
Fragments arranged by size:
554 220 201
DraI TTT'AAA
Cuts at: 0 96 270 923 975
Size: 96 174 653 52
Fragments arranged by size:
653 174 96 52
EaeI y'GGCC_r
Cuts at: 0 817 975
Size: 817 158
EcoNI CCTnn'n_nnAGG
Cuts at: 0 75 975
Size: 75 900
EcoRI G'AATT_C
Cuts at: 0 840 975
Size: 840 135
HaeII r_GCGC'y
Cuts at: 0 9 975
Size: 9 966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 697 729 975
Size: 697 32 246
Fragments arranged by size:
697 246 32
KpnI G_GTAC'C
Cuts at: 0 430 975
Size: 430 545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 975
Size: 184 791
MscI TGG'CCA
Cuts at: 0 819 975
Size: 819 156
MslI CAynn'nnrTG
Cuts at: 0 518 767 975
Size: 518 249 208
Fragments arranged by size:
518 249 208
MspA1I CmG'CkG
Cuts at: 0 24 975
Size: 24 951
NdeI CA'TA_TG
Cuts at: 0 615 975
Size: 615 360
NspI r_CATG'y
Cuts at: 0 185 975
Size: 185 790
PciI A'CATG_T
Cuts at: 0 181 975
Size: 181 794
SfcI C'TryA_G
Cuts at: 0 470 975
Size: 470 505
SspI AAT'ATT
Cuts at: 0 965 975
Size: 965 10
Enzymes that do cut:
Acc65I AclI AfeI AflIII AlwNI ApoI BanI BanII
BciVI BglII BmrI BplI BsaWI BseRI BsgI BsmI
Bsp1286I BspEI BsrDI BssSI BstAPI BstXI BstYI BtgZI
BtsI DraI EaeI EcoNI EcoRI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NdeI NspI PciI SfcI
SspI
Enzymes that do not cut:
AarI AatII AccI AcuI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI