################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HinfI HpaI HphI KasI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AgeI 7 ACCGGT A^CCGGT AlwI 622 GATCC GGATC(4/5) Asp700I 659 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 175 GGCACC G^G[CT][GA]CC BcgI 26 GCAGCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 32 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 819 AGATCT A^GATCT BpmI 58 CTGGAG CTGGAG(16/14) BsaI 935 GAGACC GGTCTC(1/5) BsgI 356 CTGCAC GTGCAG(16/14) BspMI 240 ACCTGC ACCTGC(4/8) BsrBI 556 GAGCGG CCGCTC(-3/-3) BstXI 485 CCACCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 420 GGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 185 CGGCCG [CT]^GGCC[GA] EagI 185 CGGCCG C^GGCCG EarI 456 GAAGAG CTCTTC(1/4) EclXI 185 CGGCCG C^GGCCG Eco52I 185 CGGCCG C^GGCCG EcoO109I 420 GGGCCCC [GA]G^G[ACGT]CC[CT] HaeII 479 GGCGCT [GA]GCGC^[CT] HincII 496 GTCAAC GT[CT]^[GA]AC HindII 496 GTCAAC GT[CT]^[GA]AC Ksp632I 456 GAAGAG CTCTTC(1/4) MaeIII 374 GTGAC ^GT[ACGT]AC MseI 928 TTAA T^TAA MslI 486 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 288 CATG CATG^ NotI 184 GCGGCCGC GC^GGCCGC PflFI 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 7 ACCGGT A^CCGGT PvuII 1009 CAGCTG CAG^CTG SfcI 643 CTACAG C^T[GA][CT]AG SgrAI 486 CACCGGCG C[GA]^CCGG[CT]G SmlI 711 CTCAAG C^T[CT][GA]AG Tru9I 928 TTAA T^TAA Tsp45I 374 GTGAC ^GT[GC]AC Tth111I 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 47 CCACAGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 659 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 142 GACGCC G[GA]^CG[CT]C 394 GGCGTC AfaI 528 GTAC GT^AC 779 GTAC ApaI 111 GGGCCC GGGCC^C 420 GGGCCC AspHI 202 GAGCTC G[AT]GC[AT]^C 414 GAGCTC AvaII 20 GGACC G^G[AT]CC 253 GGTCC BsaHI 142 GACGCC G[GA]^CG[CT]C 394 GGCGTC BsaWI 7 ACCGGT [AT]^CCGG[AT] 17 ACCGGA BsiHKAI 202 GAGCTC G[AT]GC[AT]^C 414 GAGCTC BsrFI 7 ACCGGT [GA]^CCGG[CT] 487 ACCGGC BssHII 90 GCGCGC G^CGCGC 227 GCGCGC BstF5I 501 CATCC GGATG(2/0) 762 GGATG BstYI 621 AGATCC [GA]^GATC[CT] 819 AGATCT Cfr10I 7 ACCGGT [GA]^CCGG[CT] 487 ACCGGC DdeI 519 CTTAG C^T[ACGT]AG 859 CTAAG DsaI 161 CCGCGG C^C[GA][CT]GG 313 CCGCGG FokI 501 CATCC GGATG(9/13) 762 GGATG Hin1I 142 GACGCC G[GA]^CG[CT]C 394 GGCGTC KspI 161 CCGCGG CCGC^GG 313 CCGCGG MflI 621 AGATCC [GA]^GATC[CT] 819 AGATCT RsaI 528 GTAC GT^AC 779 GTAC SacI 202 GAGCTC GAGCT^C 414 GAGCTC SacII 161 CCGCGG CCGC^GG 313 CCGCGG TaqI 452 TCGA T^CGA 726 TCGA TthHB8I 452 TCGA T^CGA 726 TCGA XhoII 621 AGATCC [GA]^GATC[CT] 819 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AluI AvaI BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI SmaI TseI Tsp509I TspRI XmaCI XmaI