(Circular) (Six-base) MAPSORT of: pFN21AB7673.seq Check: 2468 from: 1 to: 5725 pFN21AB7673 5725 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2254 2254 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2724 3788 5172 5440 1632 Size: 1092 1064 1384 268 1917 Fragments arranged by size: 1917 1384 1092 1064 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 2012 4556 2012 Size: 2544 3181 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2495 1166 Size: 1329 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaI G_GGCC'C Cuts at: 2120 2429 2120 Size: 309 5416 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2128 2224 2261 2422 3050 1870 Size: 73 170 15 96 37 161 628 4545 Fragments arranged by size: 4545 628 170 161 96 73 37 15 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2775 2808 2775 Size: 33 5692 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 3046 4501 618 Size: 325 217 627 393 866 1455 1842 Fragments arranged by size: 1842 1455 866 627 393 325 217 BanII G_rGCy'C Cuts at: 1391 2120 2176 2211 2340 2423 2429 3044 1391 Size: 729 56 35 129 83 6 615 4072 Fragments arranged by size: 4072 729 615 129 83 56 35 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2020 2054 3916 3950 1903 Size: 34 83 34 1862 34 3678 Fragments arranged by size: 3678 1862 83 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 4336 136 Size: 107 122 71 631 605 371 2293 1525 Fragments arranged by size: 2293 1525 631 605 371 122 107 71 BglII A'GATC_T Cuts at: 2824 5720 2824 Size: 2896 2829 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2120 2183 2429 3786 4942 1790 Size: 330 63 246 1357 1156 2573 Fragments arranged by size: 2573 1357 1156 330 246 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2424 3456 4414 Size: 416 53 83 399 24 1007 1032 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1032 1007 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4385 1239 Size: 845 2301 2579 Fragments arranged by size: 2579 2301 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2701 3850 4715 5013 2701 Size: 1149 865 298 3413 Fragments arranged by size: 3413 1149 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2934 4388 882 Size: 617 1435 1454 2219 Fragments arranged by size: 2219 1454 1435 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2400 3532 3914 Size: 53 83 186 936 615 252 1132 382 Cuts at: 3914 4549 275 Size: 635 1451 Fragments arranged by size: 1451 1132 936 635 615 382 252 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 2022 4158 4556 4830 4977 5388 2012 Size: 10 2136 398 274 147 411 2349 Fragments arranged by size: 2349 2136 411 398 274 147 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2399 2402 1186 Size: 895 318 3 4509 Fragments arranged by size: 4509 895 318 3 BseYI C'CCAG_C Cuts at: 2153 2341 2373 4928 5424 2153 Size: 188 32 2555 496 2454 Fragments arranged by size: 2555 2454 496 188 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2346 1163 Size: 573 610 4542 Fragments arranged by size: 4542 610 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2193 2283 2327 3175 3936 Size: 275 186 12 180 90 44 848 761 Cuts at: 3936 4964 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 848 761 275 186 180 90 44 12 BsiHKAI G_wGCw'C Cuts at: 2211 2423 3786 3871 4942 2211 Size: 212 1363 85 1071 2994 Fragments arranged by size: 2994 1363 1071 212 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2176 2183 2211 2340 2423 2429 Size: 399 330 56 7 28 129 83 6 Cuts at: 2429 3044 3786 3871 4942 1391 Size: 615 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 399 330 129 85 83 56 28 7 6 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2254 3080 844 Size: 1410 826 3489 Fragments arranged by size: 3489 1410 826 BsrBI CCG'CTC Cuts at: 1394 1823 2563 3612 1394 Size: 429 740 1049 3507 Fragments arranged by size: 3507 1049 740 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 2492 4369 4556 1700 Size: 110 202 480 1877 187 2869 Fragments arranged by size: 2869 1877 480 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 2095 2232 1925 Size: 170 137 5418 Fragments arranged by size: 5418 170 137 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstXI CCAn_nnnn'nTGG Cuts at: 2497 2497 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2626 2824 3055 3422 3807 3824 5276 5362 Size: 654 198 231 367 385 17 1452 86 Cuts at: 5362 5374 5720 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 654 385 367 346 231 198 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 2318 513 Size: 552 939 162 152 3920 Fragments arranged by size: 3920 939 552 162 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 3241 4035 4055 3241 Size: 794 20 4911 Fragments arranged by size: 4911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 3172 4061 Size: 54 1425 50 275 18 360 982 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 982 889 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 3172 1537 Size: 275 378 982 4090 Fragments arranged by size: 4090 982 378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 3655 1353 Size: 1103 1199 3423 Fragments arranged by size: 3423 1199 1103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 4312 4686 4832 387 Size: 1064 138 689 2034 374 146 1280 Fragments arranged by size: 2034 1280 1064 689 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 2421 2209 Size: 212 5513 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2724 3788 4385 5172 5440 1239 Size: 393 452 640 1064 597 787 268 1524 Fragments arranged by size: 1524 1064 787 640 597 452 393 268 EcoO109I rG'GnC_Cy Cuts at: 2426 2426 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2488 4872 1678 Size: 810 2384 2531 Fragments arranged by size: 2531 2384 810 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 3069 3315 669 Size: 988 281 565 566 246 3079 Fragments arranged by size: 3079 988 566 565 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2307 4839 5023 1305 Size: 1002 2532 184 2007 Fragments arranged by size: 2532 2007 1002 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3903 Fragments arranged by size: 3903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3683 4042 4201 518 Size: 648 1329 1188 359 159 2042 Fragments arranged by size: 2042 1329 1188 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2153 2168 2198 2320 3016 3141 3818 Size: 428 119 15 30 122 696 125 677 Cuts at: 3818 4966 5211 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 428 245 125 122 119 30 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 2190 3172 2190 Size: 982 4743 NspI r_CATG'y Cuts at: 1132 1229 3083 4628 1132 Size: 97 1854 1545 2229 Fragments arranged by size: 2229 1854 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5725 PfoI T'CCnGG_A Cuts at: 2061 2061 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PspOMI G'GGCC_C Cuts at: 2116 2425 2116 Size: 309 5416 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 3016 3016 Size: 5725 SacI G_AGCT'C Cuts at: 2211 2423 2211 Size: 212 5513 SacII CC_GC'GG Cuts at: 2169 2321 2169 Size: 152 5573 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2648 3073 4208 4889 5080 826 Size: 219 1603 425 1135 681 191 1471 Fragments arranged by size: 1603 1471 1135 681 425 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 2492 1700 Size: 792 4933 SmaI CCC'GGG Cuts at: 1872 2115 2130 2226 2263 3052 1872 Size: 243 15 96 37 789 4545 Fragments arranged by size: 4545 789 243 96 37 15 SmlI C'TyrA_G Cuts at: 820 1017 1943 2716 3829 4730 4992 820 Size: 197 926 773 1113 901 262 1553 Fragments arranged by size: 1553 1113 926 901 773 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 513 Size: 552 939 1160 3074 Fragments arranged by size: 3074 1160 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 5685 5717 2235 Size: 32 3418 32 2243 Fragments arranged by size: 3418 2243 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 4642 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2059 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 2113 2128 2224 2261 3050 1870 Size: 243 15 96 37 789 4545 Fragments arranged by size: 4545 789 243 96 37 15 XmnI GAAnn'nnTTC Cuts at: 2668 3854 2668 Size: 1186 4539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI