################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DpnII DraI DraII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AgeI 118 ACCGGT A^CCGGT AluI 189 AGCT AG^CT AvrII 238 CCTAGG C^CTAGG BalI 247 TGGCCA TGG^CCA BanII 124 GAGCCC G[GA]GC[CT]^C BbsI 149 GAAGAC GAAGAC(2/6) BfaI 239 CTAG C^TAG BlnI 238 CCTAGG C^CTAGG BmyI 124 GAGCCC G[AGT]GC[ACT]^C BpmI 212 CTGGAG CTGGAG(16/14) BpuAI 149 GAAGAC GAAGAC(2/6) BsaI 138 GAGACC GGTCTC(1/5) BsaWI 118 ACCGGT [AT]^CCGG[AT] BsiEI 87 CGGCCG CG[GA][CT]^CG Bsp1286I 124 GAGCCC G[AGT]GC[ACT]^C BspMI 54 ACCTGC ACCTGC(4/8) BsrFI 118 ACCGGT [GA]^CCGG[CT] BsrI 211 ACTGG ACTGG(1/-1) BstF5I 299 CATCC GGATG(2/0) Cfr10I 118 ACCGGT [GA]^CCGG[CT] DraIII 8 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 87 CGGCCG C^GGCCG EclXI 87 CGGCCG C^GGCCG Eco52I 87 CGGCCG C^GGCCG EcoT14I 238 CCTAGG C^C[AT][AT]GG FauI 22 GCGGG CCCGC(4/6) FokI 299 CATCC GGATG(9/13) HaeII 66 AGCGCC [GA]GCGC^[CT] HapII 119 CCGG C^CGG HgaI 83 GACGC GACGC(5/10) HpaII 119 CCGG C^CGG MaeI 239 CTAG C^TAG MluNI 247 TGGCCA TGG^CCA MscI 247 TGGCCA TGG^CCA MspI 119 CCGG C^CGG NotI 86 GCGGCCGC GC^GGCCGC PinAI 118 ACCGGT A^CCGGT StyI 238 CCTAGG C^C[AT][AT]GG TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BsmAI 138 GAGAC GTCTC(1/5) 295 GAGAC BsmFI 48 GGGAC GGGAC(10/14) 176 GTCCC Cac8I 7 GCACGC GC[ACGT]^[ACGT]GC 273 GCCTGC HinfI 174 GAGTC G^A[ACGT]TC 310 GAGTC HphI 13 GGTGA GGTGA(8/7) 121 GGTGA MwoI 11 GCGGTGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 58 GCCGCCCCAGC PleI 174 GAGTC GAGTC(4/5) 310 GAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AccII AciI BbvI BsaJI BssKI BstNI BstUI CfoI DdeI EaeI EarI EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI Ksp632I MboII MnlI MvaI MvnI ScrFI TseI