Restriction map for
pFN21AE4066
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BclI BfaI BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 733 GTCTAC GT^[AC][GT]AC
AflII 366 CTTAAG C^TTAAG
AhdI 277 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 277 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 120 GGCGCC G^G[CT][GA]CC
BbeI 120 GGCGCC GGCGC^C
BbvCI 928 CCTCAGC CCTCAGC(-5/-2)
BfrI 366 CTTAAG C^TTAAG
BglII 662 AGATCT A^GATCT
BlpI 288 GCTCAGC GC^T[ACGT]AGC
BpmI 447 CTGGAG CTGGAG(16/14)
Bpu1102I 288 GCTCAGC GC^T[ACGT]AGC
BsgI 399 CTGCAC GTGCAG(16/14)
BsmAI 9 GTCTC GTCTC(1/5)
BspHI 1019 TCATGA T^CATGA
BsrFI 205 GCCGGC [GA]^CCGG[CT]
BssHII 269 GCGCGC G^CGCGC
BssSI 885 CACGAG CACGAG(-5/-1)
CelII 288 GCTCAGC GC^T[ACGT]AGC
Cfr10I 205 GCCGGC [GA]^CCGG[CT]
Eam1105I 277 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 577 CTCTTC CTCTTC(1/4)
HinfI 311 GACTC G^A[ACGT]TC
KasI 120 GGCGCC G^GCGCC
Ksp632I 577 CTCTTC CTCTTC(1/4)
MaeIII 1027 GTCAC ^GT[ACGT]AC
MseI 367 TTAA T^TAA
MslI 506 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 205 GCCGGC GCC^GGC
NarI 120 GGCGCC GG^CGCC
NgoMIV 205 GCCGGC G^CCGGC
PflMI 477 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 311 GACTC GAGTC(4/5)
PstI 508 CTGCAG CTGCA^G
RcaI 1019 TCATGA T^CATGA
SapI 576 GCTCTTC GCTCTTC(1/4)
ScaI 170 AGTACT AGT^ACT
SexAI 476 ACCAGGT A^CC[AT]GGT
SfoI 120 GGCGCC GGC^GCC
SphI 911 GCATGC GCATG^C
StuI 705 AGGCCT AGG^CCT
TaqI 539 TCGA T^CGA
Tru9I 367 TTAA T^TAA
Tsp45I 1027 GTCAC ^GT[GC]AC
Tsp509I 787 AATT ^AATT
TthHB8I 539 TCGA T^CGA
Van91I 477 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 120 GGCGCC G[GA]^CG[CT]C
277 GACGCC
AfaI 171 GTAC GT^AC
474 GTAC
AlwNI 478 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
646 CAGGATCTG
Aor51HI 749 AGCGCT AGC^GCT
932 AGCGCT
AspHI 361 GAGCTC G[AT]GC[AT]^C
457 GAGCTC
BglI 98 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
200 GCCTGGCCGGC
BsaHI 120 GGCGCC G[GA]^CG[CT]C
277 GACGCC
BsiHKAI 361 GAGCTC G[AT]GC[AT]^C
457 GAGCTC
BsmFI 146 GGGAC GGGAC(10/14)
829 GGGAC
BstXI 505 CCACTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCATAGTTCTGG
DsaI 187 CCGCGG C^C[GA][CT]GG
220 CCGCGG
Eco47III 749 AGCGCT AGC^GCT
932 AGCGCT
EcoT14I 800 CCAAGG C^C[AT][AT]GG
960 CCTTGG
HgaI 277 GACGC GACGC(5/10)
918 GACGC
Hin1I 120 GGCGCC G[GA]^CG[CT]C
277 GACGCC
KspI 187 CCGCGG CCGC^GG
220 CCGCGG
MboII 578 TCTTC GAAGA(8/7)
743 TCTTC
PpuMI 479 AGGTCCT [GA]G^G[AT]CC[CT]
779 AGGTCCT
PvuII 899 CAGCTG CAG^CTG
967 CAGCTG
RsaI 171 GTAC GT^AC
474 GTAC
SacI 361 GAGCTC GAGCT^C
457 GAGCTC
SacII 187 CCGCGG CCGC^GG
220 CCGCGG
SfcI 508 CTGCAG C^T[GA][CT]AG
1031 CTACAG
SmlI 4 CTCAAG C^T[CT][GA]AG
366 CTTAAG
StyI 800 CCAAGG C^C[AT][AT]GG
960 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SfaNI SmaI TseI
TspRI XhoII XmaCI XmaI