(Circular) (Six-base) MAPSORT of: pFN21AE4066.seq Check: 4472 from: 1 to: 5743 pFN21AE4066 5743 bp With 182 enzymes: SgfI * March 28, 2012 13:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2739 3086 1937 Size: 802 347 4594 Fragments arranged by size: 4594 802 347 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2616 3806 5190 5458 1632 Size: 431 553 1190 1384 268 1917 Fragments arranged by size: 1917 1384 1190 553 431 268 AfeI AGC'GCT Cuts at: 2756 2939 2756 Size: 183 5560 AflII C'TTAA_G Cuts at: 820 1017 2371 820 Size: 197 1354 4192 Fragments arranged by size: 4192 1354 197 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 2287 4472 2287 Size: 2185 3558 AleI CACnn'nnGTG Cuts at: 1166 2515 1166 Size: 1349 4394 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2488 2656 3486 5058 2488 Size: 168 830 1572 3173 Fragments arranged by size: 3173 1572 830 168 ApaI G_GGCC'C Cuts at: 2200 2354 2876 2200 Size: 154 522 5067 Fragments arranged by size: 5067 522 154 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2094 2148 2199 2269 3068 1870 Size: 73 133 18 54 51 70 799 4545 Fragments arranged by size: 4545 799 133 73 70 54 51 18 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 3064 4519 618 Size: 325 217 627 338 939 1455 1842 Fragments arranged by size: 1842 1455 939 627 338 325 217 BanII G_rGCy'C Cuts at: 1391 2200 2354 2370 2450 2466 2579 2876 3062 Size: 809 154 16 80 16 113 297 186 Cuts at: 3062 1391 Size: 4072 Fragments arranged by size: 4072 809 297 186 154 113 80 16 16 BbeI G_GCGC'C Cuts at: 2129 2129 Size: 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4772 BbvCI CC'TCA_GC Cuts at: 2934 2934 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3934 3968 1903 Size: 34 50 34 1913 34 3678 Fragments arranged by size: 3678 1913 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2211 4354 Size: 107 122 71 631 605 437 102 2143 Cuts at: 4354 136 Size: 1525 Fragments arranged by size: 2143 1525 631 605 437 122 107 102 71 BglII A'GATC_T Cuts at: 2667 5738 2667 Size: 3071 2672 BlpI GC'TnA_GC Cuts at: 2294 3160 2294 Size: 866 4877 Bme1580I G_kGCm'C Cuts at: 1790 2200 2354 2876 3804 4960 1790 Size: 410 154 522 928 1156 2573 Fragments arranged by size: 2573 1156 928 522 410 154 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2473 4403 1239 Size: 1234 1930 2579 Fragments arranged by size: 2579 1930 1234 Bpu10I CC'TnA_GC Cuts at: 2934 2934 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1994 3868 4733 5031 1994 Size: 1874 865 298 2706 Fragments arranged by size: 2706 1874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 2283 3550 3932 Size: 53 83 186 936 593 157 1267 382 Cuts at: 3932 4567 275 Size: 635 1451 Fragments arranged by size: 1451 1267 936 635 593 382 186 157 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5518 Fragments arranged by size: 5518 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2186 2722 2832 1186 Size: 997 3 536 110 4097 Fragments arranged by size: 4097 997 536 110 3 BseYI C'CCAG_C Cuts at: 2121 2902 2910 4946 5442 2121 Size: 781 8 2036 496 2422 Fragments arranged by size: 2422 2036 781 496 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 1163 Size: 573 653 4517 Fragments arranged by size: 4517 653 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2466 3804 3889 4960 2370 Size: 96 1338 85 1071 3153 Fragments arranged by size: 3153 1338 1071 96 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2354 2370 2450 2466 2579 2876 Size: 399 410 154 16 80 16 113 297 Cuts at: 2876 3062 3804 3889 4960 1391 Size: 186 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 297 186 154 113 85 80 16 16 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3024 3632 5362 3024 Size: 608 1730 3405 Fragments arranged by size: 3405 1730 608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 844 Size: 2254 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 4387 4574 1700 Size: 110 400 2177 187 2869 Fragments arranged by size: 2869 2177 400 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 2274 1925 Size: 349 5394 BssSI C'ACGA_G Cuts at: 2890 3803 4815 2890 Size: 913 1012 3818 Fragments arranged by size: 3818 1012 913 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2517 2572 2517 Size: 55 5688 BstYI r'GATC_y Cuts at: 1972 2337 2653 2667 3073 3440 3825 3842 5294 Size: 365 316 14 406 367 385 17 1452 Cuts at: 5294 5380 5392 5738 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 406 385 367 365 346 316 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2192 2225 513 Size: 552 1127 33 4031 Fragments arranged by size: 4031 1127 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2510 2522 2772 2783 3259 4053 4073 2510 Size: 12 250 11 476 794 20 4180 Fragments arranged by size: 4180 794 476 250 20 12 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2978 3010 Size: 54 1425 50 275 18 378 770 32 Cuts at: 3010 3190 4079 5708 8 Size: 180 889 1629 43 Fragments arranged by size: 1629 1425 889 770 378 275 180 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2588 3673 1353 Size: 1235 1085 3423 Fragments arranged by size: 3423 1235 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2368 2464 2368 Size: 96 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2473 2616 3806 4403 5190 5458 Size: 393 431 410 143 1190 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 1190 787 597 431 410 393 268 143 EcoO109I rG'GnC_Cy Cuts at: 2203 2350 2485 2785 2852 2873 2203 Size: 147 135 300 67 21 5073 Fragments arranged by size: 5073 300 147 135 67 21 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2129 2758 2941 4890 1678 Size: 451 629 183 1949 2531 Fragments arranged by size: 2531 1949 629 451 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 3006 3038 4384 Size: 32 135 32 701 32 701 32 1346 Cuts at: 4384 4416 4458 4490 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1346 701 701 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2125 2125 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 4857 5041 1305 Size: 861 2691 184 2007 Fragments arranged by size: 2691 2007 861 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2515 3701 4060 4219 518 Size: 648 1349 1186 359 159 2042 Fragments arranged by size: 2042 1349 1186 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 2227 2260 2313 2385 2430 2906 2974 Size: 588 33 33 53 72 45 476 68 Cuts at: 2974 3159 3836 4984 5229 1606 Size: 185 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 588 476 245 185 72 68 53 45 33 33 NaeI GCC'GGC Cuts at: 2212 2212 Size: 5743 NarI GG'CG_CC Cuts at: 2126 2126 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NgoMIV G'CCGG_C Cuts at: 2210 2210 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2029 2683 2920 3101 4646 1132 Size: 97 800 654 237 181 1545 2229 Fragments arranged by size: 2229 1545 800 654 237 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 4363 PfoI T'CCnGG_A Cuts at: 2450 2450 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2485 2785 2485 Size: 300 5443 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2196 2350 2872 2196 Size: 154 522 5067 Fragments arranged by size: 5067 522 154 PstI C_TGCA'G Cuts at: 830 2517 3095 830 Size: 1687 578 3478 Fragments arranged by size: 3478 1687 578 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2906 2974 2906 Size: 68 5675 SacI G_AGCT'C Cuts at: 2370 2466 2370 Size: 96 5647 SacII CC_GC'GG Cuts at: 2195 2228 2195 Size: 33 5710 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SapI GCTCTTCn'nnn_ Cuts at: 2588 2588 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2177 3991 5703 2177 Size: 1814 1712 2217 Fragments arranged by size: 2217 1814 1712 SexAI A'CCwGG_T Cuts at: 2481 2481 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 2513 3036 3091 4226 4907 5098 826 Size: 219 1468 523 55 1135 681 191 1471 Fragments arranged by size: 1471 1468 1135 681 523 219 191 55 SfoI GGC'GCC Cuts at: 2127 2127 Size: 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2078 2096 2150 2201 2271 3070 1872 Size: 206 18 54 51 70 799 4545 Fragments arranged by size: 4545 799 206 70 54 51 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 2009 2371 3847 4748 5010 820 Size: 197 926 66 362 1476 901 262 1553 Fragments arranged by size: 1553 1476 926 901 362 262 197 66 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 2920 3101 1132 Size: 1788 181 3774 Fragments arranged by size: 3774 1788 181 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2712 1517 Size: 1195 4548 StyI C'CwwG_G Cuts at: 513 1065 2805 2965 3182 513 Size: 552 1740 160 217 3074 Fragments arranged by size: 3074 1740 552 217 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3989 5701 Size: 274 80 33 51 1443 198 1814 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1814 1712 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2076 2094 2148 2199 2269 3068 1870 Size: 206 18 54 51 70 799 4545 Fragments arranged by size: 4545 799 206 70 54 51 18 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI