(Linear) (Six-base) MAPSORT of: KIEE4066.seq Check: 4569 from: 1 to: 1038 KIEE4066.orf With 182 enzymes: SgfI * March 22, 2012 17:18 .. AccI GT'mk_AC Cuts at: 0 734 1038 Size: 734 304 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 611 1038 Size: 58 553 427 Fragments arranged by size: 553 427 58 AfeI AGC'GCT Cuts at: 0 751 934 1038 Size: 751 183 104 Fragments arranged by size: 751 183 104 AflII C'TTAA_G Cuts at: 0 366 1038 Size: 366 672 AhdI GACnn_n'nnGTC Cuts at: 0 282 1038 Size: 282 756 AleI CACnn'nnGTG Cuts at: 0 510 1038 Size: 510 528 AlwNI CAG_nnn'CTG Cuts at: 0 483 651 1038 Size: 483 168 387 Fragments arranged by size: 483 387 168 ApaI G_GGCC'C Cuts at: 0 195 349 871 1038 Size: 195 154 522 167 Fragments arranged by size: 522 195 167 154 AvaI C'yCGr_G Cuts at: 0 71 89 143 194 264 1038 Size: 71 18 54 51 70 774 Fragments arranged by size: 774 71 70 54 51 18 BanI G'GyrC_C Cuts at: 0 120 1038 Size: 120 918 BanII G_rGCy'C Cuts at: 0 195 349 365 445 461 574 871 1038 Size: 195 154 16 80 16 113 297 167 Fragments arranged by size: 297 195 167 154 113 80 16 16 BbeI G_GCGC'C Cuts at: 0 124 1038 Size: 124 914 BbvCI CC'TCA_GC Cuts at: 0 929 1038 Size: 929 109 BglI GCCn_nnn'nGGC Cuts at: 0 104 206 1038 Size: 104 102 832 Fragments arranged by size: 832 104 102 BglII A'GATC_T Cuts at: 0 662 1038 Size: 662 376 BlpI GC'TnA_GC Cuts at: 0 289 1038 Size: 289 749 Bme1580I G_kGCm'C Cuts at: 0 195 349 871 1038 Size: 195 154 522 167 Fragments arranged by size: 522 195 167 154 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 459 1038 Size: 459 579 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 468 1038 Size: 468 570 Bpu10I CC'TnA_GC Cuts at: 0 929 1038 Size: 929 109 BsaHI Gr'CG_yC Cuts at: 0 121 278 1038 Size: 121 157 760 Fragments arranged by size: 760 157 121 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 178 181 717 827 1038 Size: 178 3 536 110 211 Fragments arranged by size: 536 211 178 110 3 BseYI C'CCAG_C Cuts at: 0 116 897 905 1038 Size: 116 781 8 133 Fragments arranged by size: 781 133 116 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 1038 Size: 384 654 BsiHKAI G_wGCw'C Cuts at: 0 365 461 1038 Size: 365 96 577 Fragments arranged by size: 577 365 96 Bsp1286I G_dGCh'C Cuts at: 0 195 349 365 445 461 574 871 1038 Size: 195 154 16 80 16 113 297 167 Fragments arranged by size: 297 195 167 154 113 80 16 16 BspHI T'CATG_A Cuts at: 0 1019 1038 Size: 1019 19 BsrFI r'CCGG_y Cuts at: 0 205 1038 Size: 205 833 BssHII G'CGCG_C Cuts at: 0 269 1038 Size: 269 769 BssSI C'ACGA_G Cuts at: 0 885 1038 Size: 885 153 BstXI CCAn_nnnn'nTGG Cuts at: 0 512 567 1038 Size: 512 55 471 Fragments arranged by size: 512 471 55 BstYI r'GATC_y Cuts at: 0 332 648 662 1038 Size: 332 316 14 376 Fragments arranged by size: 376 332 316 14 BtgI C'CryG_G Cuts at: 0 187 220 1038 Size: 187 33 818 Fragments arranged by size: 818 187 33 BtsI GCAGTG_nn' Cuts at: 0 505 517 767 778 1038 Size: 505 12 250 11 260 Fragments arranged by size: 505 260 250 12 11 EaeI y'GGCC_r Cuts at: 0 203 973 1005 1038 Size: 203 770 32 33 Fragments arranged by size: 770 203 33 32 EarI CTCTTCn'nnn_ Cuts at: 0 583 1038 Size: 583 455 EcoICRI GAG'CTC Cuts at: 0 363 459 1038 Size: 363 96 579 Fragments arranged by size: 579 363 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 468 611 1038 Size: 58 410 143 427 Fragments arranged by size: 427 410 143 58 EcoO109I rG'GnC_Cy Cuts at: 0 198 345 480 780 847 868 1038 Size: 198 147 135 300 67 21 170 Fragments arranged by size: 300 198 170 147 135 67 21 HaeII r_GCGC'y Cuts at: 0 124 753 936 1038 Size: 124 629 183 102 Fragments arranged by size: 629 183 124 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 268 300 1001 1033 1038 Size: 268 32 701 32 5 Fragments arranged by size: 701 268 32 32 5 KasI G'GCGC_C Cuts at: 0 120 1038 Size: 120 918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 161 1038 Size: 161 877 MslI CAynn'nnrTG Cuts at: 0 510 1038 Size: 510 528 MspA1I CmG'CkG Cuts at: 0 189 222 255 308 380 425 901 969 Size: 189 33 33 53 72 45 476 68 Cuts at: 969 1038 Size: 69 Fragments arranged by size: 476 189 72 69 68 53 45 33 33 NaeI GCC'GGC Cuts at: 0 207 1038 Size: 207 831 NarI GG'CG_CC Cuts at: 0 121 1038 Size: 121 917 NgoMIV G'CCGG_C Cuts at: 0 205 1038 Size: 205 833 NspI r_CATG'y Cuts at: 0 24 678 915 1038 Size: 24 654 237 123 Fragments arranged by size: 654 237 123 24 PflMI CCAn_nnn'nTGG Cuts at: 0 483 1038 Size: 483 555 PfoI T'CCnGG_A Cuts at: 0 445 1038 Size: 445 593 PpuMI rG'GwC_Cy Cuts at: 0 480 780 1038 Size: 480 300 258 Fragments arranged by size: 480 300 258 PspOMI G'GGCC_C Cuts at: 0 191 345 867 1038 Size: 191 154 522 171 Fragments arranged by size: 522 191 171 154 PstI C_TGCA'G Cuts at: 0 512 1038 Size: 512 526 PvuII CAG'CTG Cuts at: 0 901 969 1038 Size: 901 68 69 Fragments arranged by size: 901 69 68 SacI G_AGCT'C Cuts at: 0 365 461 1038 Size: 365 96 577 Fragments arranged by size: 577 365 96 SacII CC_GC'GG Cuts at: 0 190 223 1038 Size: 190 33 815 Fragments arranged by size: 815 190 33 SapI GCTCTTCn'nnn_ Cuts at: 0 583 1038 Size: 583 455 ScaI AGT'ACT Cuts at: 0 172 1038 Size: 172 866 SexAI A'CCwGG_T Cuts at: 0 476 1038 Size: 476 562 SfcI C'TryA_G Cuts at: 0 508 1031 1038 Size: 508 523 7 Fragments arranged by size: 523 508 7 SfoI GGC'GCC Cuts at: 0 122 1038 Size: 122 916 SmaI CCC'GGG Cuts at: 0 73 91 145 196 266 1038 Size: 73 18 54 51 70 772 Fragments arranged by size: 772 73 70 54 51 18 SmlI C'TyrA_G Cuts at: 0 4 366 1038 Size: 4 362 672 Fragments arranged by size: 672 362 4 SphI G_CATG'C Cuts at: 0 915 1038 Size: 915 123 StuI AGG'CCT Cuts at: 0 707 1038 Size: 707 331 StyI C'CwwG_G Cuts at: 0 800 960 1038 Size: 800 160 78 Fragments arranged by size: 800 160 78 TatI w'GTAC_w Cuts at: 0 170 1038 Size: 170 868 XmaI C'CCGG_G Cuts at: 0 71 89 143 194 264 1038 Size: 71 18 54 51 70 774 Fragments arranged by size: 774 71 70 54 51 18 Enzymes that do cut: AccI AcuI AfeI AflII AhdI AleI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI BssHII BssSI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TatI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI