(Circular) (Six-base) MAPSORT of: pFN21AE3459.seq Check: 3759 from: 1 to: 8731 pFN21AE3459 8731 bp With 182 enzymes: SgfI * February 23, 2012 14:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2017 2017 Size: 8731 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6541 278 Size: 53 83 186 936 5005 2468 Fragments arranged by size: 5005 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 2796 4824 6074 1937 Size: 397 462 2028 1250 4594 Fragments arranged by size: 4594 2028 1250 462 397 Acc65I G'GTAC_C Cuts at: 6052 6052 Size: 8731 AclI AA'CG_TT Cuts at: 1563 6858 7231 1563 Size: 5295 373 3063 Fragments arranged by size: 5295 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3214 3362 4779 5523 5589 6794 8178 8446 Size: 1582 148 1417 744 66 1205 1384 268 Cuts at: 8446 1632 Size: 1917 Fragments arranged by size: 1917 1582 1417 1384 1205 744 268 148 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8534 AflIII A'CryG_T Cuts at: 1203 7630 8607 1203 Size: 6427 977 1327 Fragments arranged by size: 6427 1327 977 AgeI A'CCGG_T Cuts at: 7562 7562 Size: 8731 AhdI GACnn_n'nnGTC Cuts at: 7460 7460 Size: 8731 AleI CACnn'nnGTG Cuts at: 1166 4677 1166 Size: 3511 5220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8731 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3188 3220 3188 Size: 32 8699 AlwNI CAG_nnn'CTG Cuts at: 2032 2149 2550 2916 3011 3480 3545 4435 5219 Size: 117 401 366 95 469 65 890 784 Cuts at: 5219 5315 5858 5966 6474 8046 2032 Size: 96 543 108 508 1572 2717 Fragments arranged by size: 2717 1572 890 784 543 508 469 401 366 117 108 96 95 65 ApaI G_GGCC'C Cuts at: 2667 3539 3753 3800 2667 Size: 872 214 47 7598 Fragments arranged by size: 7598 872 214 47 ApaLI G'TGCA_C Cuts at: 4172 6788 7944 4172 Size: 2616 1156 4959 Fragments arranged by size: 4959 2616 1156 ApoI r'AATT_y Cuts at: 1493 2282 3629 6040 6266 6418 8527 1493 Size: 789 1347 2411 226 152 2109 1697 Fragments arranged by size: 2411 2109 1697 1347 789 226 152 AseI AT'TA_AT Cuts at: 160 1032 7285 160 Size: 872 6253 1606 Fragments arranged by size: 6253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8731 AvaI C'yCGr_G Cuts at: 1870 1943 2055 3037 3624 3706 4153 4911 5450 Size: 73 112 982 587 82 447 758 539 Cuts at: 5450 5520 6056 1870 Size: 70 536 4545 Fragments arranged by size: 4545 982 758 587 539 536 447 112 82 73 70 AvrII C'CTAG_G Cuts at: 3784 5639 3784 Size: 1855 6876 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4910 4943 4910 Size: 33 8698 BamHI G'GATC_C Cuts at: 4188 6061 6428 4188 Size: 1873 367 6491 Fragments arranged by size: 6491 1873 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3788 4008 5235 5528 6052 Size: 325 217 627 2001 220 1227 293 524 Cuts at: 6052 7507 618 Size: 1455 1842 Fragments arranged by size: 2001 1842 1455 1227 627 524 325 293 220 217 BanII G_rGCy'C Cuts at: 1391 2063 2667 2955 3539 3713 3753 3800 4737 Size: 672 604 288 584 174 40 47 937 Cuts at: 4737 4980 5090 5328 5375 6050 1391 Size: 243 110 238 47 675 4072 Fragments arranged by size: 4072 937 675 672 604 584 288 243 238 174 110 47 47 40 BbeI G_GCGC'C Cuts at: 4012 4012 Size: 8731 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 3154 5784 928 Size: 971 711 544 2630 3875 Fragments arranged by size: 3875 2630 971 711 544 BbvCI CC'TCA_GC Cuts at: 5096 5096 Size: 8731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 6922 6956 1903 Size: 34 288 34 4663 34 3678 Fragments arranged by size: 4663 3678 288 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4661 5156 6623 7833 4661 Size: 495 1467 1210 5559 Fragments arranged by size: 5559 1467 1210 495 BclI T'GATC_A Cuts at: 1548 3096 3297 1548 Size: 1548 201 6982 Fragments arranged by size: 6982 1548 201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3641 5843 Size: 107 122 71 631 605 337 1632 2202 Cuts at: 5843 7342 136 Size: 1499 1525 Fragments arranged by size: 2202 1632 1525 1499 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4806 8726 4806 Size: 3920 4811 BlpI GC'TnA_GC Cuts at: 5322 6148 5322 Size: 826 7905 Bme1580I G_kGCm'C Cuts at: 1790 2667 3539 3689 3736 3753 3800 4176 4420 Size: 877 872 150 47 17 47 376 244 Cuts at: 4420 5240 6792 7948 1790 Size: 820 1552 1156 2573 Fragments arranged by size: 2573 1552 1156 877 872 820 376 244 150 47 47 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4074 4430 4453 Size: 416 53 83 399 24 2657 356 23 Cuts at: 4453 4533 5093 6462 7420 442 Size: 80 560 1369 958 1753 Fragments arranged by size: 2657 1753 1369 958 560 416 399 356 83 80 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2368 2512 4708 5039 5312 5754 7391 1239 Size: 1129 144 2196 331 273 442 1637 2579 Fragments arranged by size: 2579 2196 1637 1129 442 331 273 144 Bpu10I CC'TnA_GC Cuts at: 2710 4130 5096 2710 Size: 1420 966 6345 Fragments arranged by size: 6345 1420 966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2939 4499 4514 4664 5675 6856 7721 8019 Size: 726 1560 15 150 1011 1181 865 298 Cuts at: 8019 2213 Size: 2925 Fragments arranged by size: 2925 1560 1181 1011 865 726 298 150 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3108 4908 5552 7394 882 Size: 617 1609 1800 644 1842 2219 Fragments arranged by size: 2219 1842 1800 1609 644 617 BsaAI yAC'GTr Cuts at: 493 1204 5129 5945 6522 493 Size: 711 3925 816 577 2702 Fragments arranged by size: 3925 2702 816 711 577 BsaBI GATnn'nnATC Cuts at: 8725 8725 Size: 8731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4009 6538 6920 7555 Size: 53 83 186 936 2476 2529 382 635 Cuts at: 7555 275 Size: 1451 Fragments arranged by size: 2529 2476 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3165 5607 7164 7562 7836 7983 8394 3165 Size: 2442 1557 398 274 147 411 3502 Fragments arranged by size: 3502 2442 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 2754 2784 2847 Size: 30 165 30 963 30 358 30 63 Cuts at: 2847 2877 4681 4711 5324 5354 1178 Size: 30 1804 30 613 30 4555 Fragments arranged by size: 4555 1804 963 613 358 165 63 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2628 2868 3508 3594 5072 5506 5603 5626 Size: 1442 240 640 86 1478 434 97 23 Cuts at: 5626 5672 1186 Size: 46 4245 Fragments arranged by size: 4245 1478 1442 640 434 240 97 86 46 23 BseYI C'CCAG_C Cuts at: 2032 2586 3356 3531 3979 4327 5406 5662 5851 Size: 554 770 175 448 348 1079 256 189 Cuts at: 5851 7934 8430 2032 Size: 2083 496 2333 Fragments arranged by size: 2333 2083 1079 770 554 496 448 348 256 189 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3049 3838 4795 1163 Size: 573 1313 789 957 5099 Fragments arranged by size: 5099 1313 957 789 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3470 6181 6942 7970 1540 Size: 275 186 1469 2711 761 1028 2301 Fragments arranged by size: 2711 2301 1469 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 2638 3518 3727 4176 5328 6792 6877 7948 Size: 433 880 209 449 1152 1464 85 1071 Cuts at: 7948 2205 Size: 2988 Fragments arranged by size: 2988 1464 1152 1071 880 449 433 209 85 BsmI GAATG_Cn' Cuts at: 3653 3674 6242 6335 3653 Size: 21 2568 93 6049 Fragments arranged by size: 6049 2568 93 21 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2205 2638 2667 2955 3518 3539 Size: 399 273 142 433 29 288 563 21 Cuts at: 3539 3689 3713 3727 3736 3753 3800 4176 4420 Size: 150 24 14 9 17 47 376 244 Cuts at: 4420 4737 4980 5090 5240 5328 5375 6050 6792 Size: 317 243 110 150 88 47 675 742 Cuts at: 6792 6877 7948 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 563 433 399 376 317 288 273 244 243 150 150 142 110 88 85 47 47 29 24 21 17 14 9 BspEI T'CCGG_A Cuts at: 3165 8394 3165 Size: 5229 3502 BspHI T'CATG_A Cuts at: 2563 3300 3972 6620 8350 2563 Size: 737 672 2648 1730 2944 Fragments arranged by size: 2944 2648 1730 737 672 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 4584 4779 6086 844 Size: 1173 2567 195 1307 3489 Fragments arranged by size: 3489 2567 1307 1173 195 BsrBI CCG'CTC Cuts at: 1394 1823 4789 6618 1394 Size: 429 2966 1829 3507 Fragments arranged by size: 3507 2966 1829 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2297 3706 7226 7400 65 Size: 1183 1049 1409 3520 174 1396 Fragments arranged by size: 3520 1409 1396 1183 1049 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 2742 3101 7375 7562 1700 Size: 110 310 622 359 4274 187 2869 Fragments arranged by size: 4274 2869 622 359 310 187 110 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8731 BssSI C'ACGA_G Cuts at: 2267 6791 7803 2267 Size: 4524 1012 3195 Fragments arranged by size: 4524 3195 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3480 794 Size: 2686 6045 BstBI TT'CG_AA Cuts at: 7535 7535 Size: 8731 BstEII G'GTnAC_C Cuts at: 1184 3188 1184 Size: 2004 6727 BstXI CCAn_nnnn'nTGG Cuts at: 3076 4632 5083 3076 Size: 1556 451 6724 Fragments arranged by size: 6724 1556 451 BstYI r'GATC_y Cuts at: 1972 3072 3129 4188 4806 6061 6428 6813 6830 Size: 1100 57 1059 618 1255 367 385 17 Cuts at: 6830 8282 8368 8380 8726 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1255 1100 1059 618 385 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2718 4194 2718 Size: 1476 7255 BtgI C'CryG_G Cuts at: 513 1065 3678 3775 4922 4981 5267 5375 5838 Size: 552 2613 97 1147 59 286 108 463 Cuts at: 5838 513 Size: 3406 Fragments arranged by size: 3406 2613 1147 552 463 286 108 97 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2139 2596 487 Size: 683 56 913 457 6622 Fragments arranged by size: 6622 913 683 457 56 BtsI GCAGTG_nn' Cuts at: 2091 2243 2653 3598 3687 5233 5754 6247 7041 Size: 152 410 945 89 1546 521 493 794 Cuts at: 7041 7061 2091 Size: 20 3761 Fragments arranged by size: 3761 1546 945 794 521 493 410 152 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2740 2775 529 Size: 35 2176 35 6485 Fragments arranged by size: 6485 2176 35 35 DraI TTT'AAA Cuts at: 4879 6035 4879 Size: 1156 7575 DraIII CAC_nnn'GTG Cuts at: 3760 3760 Size: 8731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7738 809 Size: 507 6422 1802 Fragments arranged by size: 6422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 3209 3467 Size: 54 1425 50 275 18 441 938 258 Cuts at: 3467 4104 4287 5123 5378 6178 7067 8696 8 Size: 637 183 836 255 800 889 1629 43 Fragments arranged by size: 1629 1425 938 889 836 800 637 441 275 258 255 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3467 6178 1537 Size: 275 1655 2711 4090 Fragments arranged by size: 4090 2711 1655 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3435 5457 5868 6661 1353 Size: 2082 2022 411 793 3423 Fragments arranged by size: 3423 2082 2022 793 411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 7318 7692 7838 387 Size: 1064 138 3491 2238 374 146 1280 Fragments arranged by size: 3491 2238 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5326 5326 Size: 8731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2368 2512 3214 3362 4708 4779 5039 Size: 393 736 144 702 148 1346 71 260 Cuts at: 5039 5312 5523 5589 5754 6794 7391 8178 8446 Size: 273 211 66 165 1040 597 787 268 Cuts at: 8446 1239 Size: 1524 Fragments arranged by size: 1524 1346 1040 787 736 702 597 393 273 268 260 211 165 148 144 71 66 EcoNI CCTnn'n_nnAGG Cuts at: 2908 2908 Size: 8731 EcoO109I rG'GnC_Cy Cuts at: 2325 2591 2714 2913 3431 3535 3542 3750 3796 Size: 266 123 199 518 104 7 208 46 Cuts at: 3796 5844 5894 5908 2325 Size: 2048 50 14 5148 Fragments arranged by size: 5148 2048 518 266 208 199 123 104 50 46 14 7 EcoRI G'AATT_C Cuts at: 2282 6040 6418 2282 Size: 3758 378 4595 Fragments arranged by size: 4595 3758 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5549 5549 Size: 8731 FspI TGC'GCA Cuts at: 7237 7237 Size: 8731 HaeII r_GCGC'y Cuts at: 1678 4012 5397 5413 7878 1678 Size: 2334 1385 16 2465 2531 Fragments arranged by size: 2531 2465 2334 1385 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 2885 2917 3438 Size: 32 135 32 845 32 436 32 521 Cuts at: 3438 3470 3570 3602 4173 4187 4205 4219 5324 Size: 32 100 32 571 14 18 14 1105 Cuts at: 5324 5356 5672 5704 7372 7404 7446 7478 1373 Size: 32 316 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 1105 845 571 521 436 316 135 100 42 32 32 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 5473 6075 6321 669 Size: 988 281 3535 602 246 3079 Fragments arranged by size: 3535 3079 988 602 281 246 HindIII A'AGCT_T Cuts at: 748 2465 4136 748 Size: 1717 1671 5343 Fragments arranged by size: 5343 1717 1671 HpaI GTT'AAC Cuts at: 6321 6321 Size: 8731 KasI G'GCGC_C Cuts at: 4008 4008 Size: 8731 KpnI G_GTAC'C Cuts at: 6056 6056 Size: 8731 MfeI C'AATT_G Cuts at: 2484 4815 6330 2484 Size: 2331 1515 4885 Fragments arranged by size: 4885 2331 1515 MluI A'CGCG_T Cuts at: 8607 8607 Size: 8731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4826 5089 7845 8029 1305 Size: 3521 263 2756 184 2007 Fragments arranged by size: 3521 2756 2007 263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 4106 4289 10 Size: 54 1425 343 441 1833 183 4452 Fragments arranged by size: 4452 1833 1425 441 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2751 4677 4832 4904 5171 5272 5681 Size: 648 1585 1926 155 72 267 101 409 Cuts at: 5681 6689 7048 7207 518 Size: 1008 359 159 2042 Fragments arranged by size: 2042 1926 1585 1008 648 409 359 267 159 155 101 72 MspA1I CmG'CkG Cuts at: 1606 2818 2871 3768 4432 4600 6147 6824 7972 Size: 1212 53 897 664 168 1547 677 1148 Cuts at: 7972 8217 1606 Size: 245 2120 Fragments arranged by size: 2120 1547 1212 1148 897 677 664 245 168 53 NaeI GCC'GGC Cuts at: 2744 2744 Size: 8731 NarI GG'CG_CC Cuts at: 4009 4009 Size: 8731 NcoI C'CATG_G Cuts at: 513 1065 3678 3775 4981 5838 513 Size: 552 2613 97 1206 857 3406 Fragments arranged by size: 3406 2613 1206 857 552 97 NdeI CA'TA_TG Cuts at: 387 4281 387 Size: 3894 4837 NgoMIV G'CCGG_C Cuts at: 2742 2742 Size: 8731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8731 NotI GC'GGCC_GC Cuts at: 6178 6178 Size: 8731 NspI r_CATG'y Cuts at: 1132 1229 3223 3699 5170 5306 6089 7634 1132 Size: 97 1994 476 1471 136 783 1545 2229 Fragments arranged by size: 2229 1994 1545 1471 783 476 136 97 PasI CC'CwG_GG Cuts at: 1348 3859 4340 4967 1348 Size: 2511 481 627 5112 Fragments arranged by size: 5112 2511 627 481 PciI A'CATG_T Cuts at: 7630 7630 Size: 8731 PflMI CCAn_nnn'nTGG Cuts at: 1108 3031 3545 3679 5159 1108 Size: 1923 514 134 1480 4680 Fragments arranged by size: 4680 1923 1480 514 134 PfoI T'CCnGG_A Cuts at: 2593 5328 2593 Size: 2735 5996 PmeI GTTT'AAAC Cuts at: 6035 6035 Size: 8731 PmlI CAC'GTG Cuts at: 5129 5129 Size: 8731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6800 6832 8313 8345 6800 Size: 32 1481 32 7186 Fragments arranged by size: 7186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2325 2591 2325 Size: 266 8465 PshAI GACnn'nnGTC Cuts at: 1613 2330 1613 Size: 717 8014 PsiI TTA'TAA Cuts at: 6301 6301 Size: 8731 PspOMI G'GGCC_C Cuts at: 2663 3535 3749 3796 2663 Size: 872 214 47 7598 Fragments arranged by size: 7598 872 214 47 PspXI vC'TCGA_Gb Cuts at: 3037 3037 Size: 8731 PstI C_TGCA'G Cuts at: 830 2028 2238 4356 5215 5761 6083 830 Size: 1198 210 2118 859 546 322 3478 Fragments arranged by size: 3478 2118 1198 859 546 322 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8731 PvuII CAG'CTG Cuts at: 2818 2871 3768 4432 4600 2818 Size: 53 897 664 168 6949 Fragments arranged by size: 6949 897 664 168 53 SacI G_AGCT'C Cuts at: 5328 5328 Size: 8731 SalI G'TCGA_C Cuts at: 1936 6073 1936 Size: 4137 4594 SanDI GG'GwC_CC Cuts at: 2325 2325 Size: 8731 SapI GCTCTTCn'nnn_ Cuts at: 3435 3435 Size: 8731 SbfI CC_TGCA'GG Cuts at: 6083 6083 Size: 8731 ScaI AGT'ACT Cuts at: 6979 8691 6979 Size: 1712 7019 SfcI C'TryA_G Cuts at: 826 1045 2024 2234 4352 5211 5757 5958 6079 Size: 219 979 210 2118 859 546 201 121 Cuts at: 6079 7214 7895 8086 826 Size: 1135 681 191 1471 Fragments arranged by size: 2118 1471 1135 979 859 681 546 219 210 201 191 121 SfoI GGC'GCC Cuts at: 4010 4010 Size: 8731 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8731 SmaI CCC'GGG Cuts at: 1872 2057 5452 5522 6058 1872 Size: 185 3395 70 536 4545 Fragments arranged by size: 4545 3395 536 185 70 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 2954 3037 3624 4478 4529 Size: 197 926 249 762 83 587 854 51 Cuts at: 4529 4643 5654 6835 7736 7998 820 Size: 114 1011 1181 901 262 1553 Fragments arranged by size: 1553 1181 1011 926 901 854 762 587 262 249 197 114 83 51 SnaBI TAC'GTA Cuts at: 493 5945 6522 493 Size: 5452 577 2702 Fragments arranged by size: 5452 2702 577 SpeI A'CTAG_T Cuts at: 152 152 Size: 8731 SphI G_CATG'C Cuts at: 1132 6089 1132 Size: 4957 3774 SspI AAT'ATT Cuts at: 5 52 4370 6655 5 Size: 47 4318 2285 2081 Fragments arranged by size: 4318 2285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8731 StyI C'CwwG_G Cuts at: 513 1065 3417 3678 3775 3784 3792 4461 4981 Size: 552 2352 261 97 9 8 669 520 Cuts at: 4981 5639 5838 6009 6170 513 Size: 658 199 171 161 3074 Fragments arranged by size: 3074 2352 669 658 552 520 261 199 171 161 97 9 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2526 3872 4552 5274 6758 6775 6928 Size: 1255 946 1346 680 722 1484 17 153 Cuts at: 6928 7113 7558 325 Size: 185 445 1498 Fragments arranged by size: 1498 1484 1346 1255 946 722 680 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4450 4470 Size: 274 80 33 51 1443 174 2299 20 Cuts at: 4470 5673 6977 8689 96 Size: 1203 1304 1712 138 Fragments arranged by size: 2299 1712 1443 1304 1203 274 174 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3948 3980 4681 4713 8691 8723 3948 Size: 32 701 32 3978 32 3956 Fragments arranged by size: 3978 3956 701 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2982 1316 Size: 1666 7065 XbaI T'CTAG_A Cuts at: 6067 6067 Size: 8731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2336 3743 4297 5186 2311 Size: 25 1407 554 889 5856 Fragments arranged by size: 5856 1407 889 554 25 XhoI C'TCGA_G Cuts at: 1943 3037 3624 1943 Size: 1094 587 7050 Fragments arranged by size: 7050 1094 587 XmaI C'CCGG_G Cuts at: 1870 2055 5450 5520 6056 1870 Size: 185 3395 70 536 4545 Fragments arranged by size: 4545 3395 536 185 70 XmnI GAAnn'nnTTC Cuts at: 2282 6860 2282 Size: 4578 4153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6539 276 Size: 53 83 186 936 5005 2468 Fragments arranged by size: 5005 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI