Restriction map for
pFN21AE4308
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbrPI
BbvCI BcgI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstUI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MflI
MluI MroI MslI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 949 GTCTAC GT^[AC][GT]AC
AcsI 535 AAATTT [GA]^AATT[CT]
AcyI 122 GGCGCC G[GA]^CG[CT]C
AfaI 732 GTAC GT^AC
AlwI 253 GATCC GGATC(4/5)
AlwNI 64 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 564 CCTGAGG CC^T[ACGT]AGG
ApoI 535 AAATTT [GA]^AATT[CT]
AspHI 913 GTGCTC G[AT]GC[AT]^C
BalI 167 TGGCCA TGG^CCA
BbeI 122 GGCGCC GGCGC^C
BbsI 91 GTCTTC GAAGAC(2/6)
BciVI 590 GGATAC GTATCC(6/5)
BclI 473 TGATCA T^GATCA
BpuAI 91 GTCTTC GAAGAC(2/6)
BsaBI 474 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 122 GGCGCC G[GA]^CG[CT]C
BseRI 216 CTCCTC GAGGAG(10/8)
BsiHKAI 913 GTGCTC G[AT]GC[AT]^C
BspHI 755 TCATGA T^CATGA
BsrDI 80 GCAATG GCAATG(2/0)
BstAPI 63 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 426 GGTCACC G^GT[ACGT]ACC
BstPI 426 GGTCACC G^GT[ACGT]ACC
Bsu36I 564 CCTGAGG CC^T[ACGT]AGG
DsaI 364 CCATGG C^C[GA][CT]GG
EaeI 167 TGGCCA [CT]^GGCC[GA]
EarI 11 CTCTTC CTCTTC(1/4)
Eco81I 564 CCTGAGG CC^T[ACGT]AGG
EcoNI 780 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 426 GGTCACC G^GT[ACGT]ACC
EcoRV 244 GATATC GAT^ATC
FbaI 473 TGATCA T^GATCA
HaeII 122 GGCGCC [GA]GCGC^[CT]
Hin1I 122 GGCGCC G[GA]^CG[CT]C
KasI 122 GGCGCC G^GCGCC
Ksp632I 11 CTCTTC CTCTTC(1/4)
MaeII 296 ACGT A^CGT
MamI 474 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 167 TGGCCA TGG^CCA
MscI 167 TGGCCA TGG^CCA
MseI 299 TTAA T^TAA
NarI 122 GGCGCC GG^CGCC
NcoI 364 CCATGG C^CATGG
NspI 807 GCATGC [GA]CATG^[CT]
PflMI 359 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 755 TCATGA T^CATGA
RsaI 732 GTAC GT^AC
SbfI 663 CCTGCAGG CCTGCA^GG
SfoI 122 GGCGCC GGC^GCC
SphI 807 GCATGC GCATG^C
Sse8387I 663 CCTGCAGG CCTGCA^GG
TaqI 822 TCGA T^CGA
Tru9I 299 TTAA T^TAA
Tsp509I 536 AATT ^AATT
TthHB8I 822 TCGA T^CGA
Van91I 359 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 533 CCAAATTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 374 CCCGGG C^[CT]CG[GA]G
461 CCCGGG
BanI 22 GGTGCC G^G[CT][GA]CC
122 GGCGCC
BglI 670 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
694 GCCACACAGGC
BmyI 677 GGGCAC G[AGT]GC[ACT]^C
913 GTGCTC
BsmAI 529 GTCTC GTCTC(1/5)
598 GTCTC
BsoBI 374 CCCGGG C^[CT]CG[GA]G
461 CCCGGG
Bsp1286I 677 GGGCAC G[AGT]GC[ACT]^C
913 GTGCTC
BspMI 200 ACCTGC ACCTGC(4/8)
887 ACCTGC
BsrFI 59 ACCGGC [GA]^CCGG[CT]
100 ACCGGC
CfoI 123 GCGC GCG^C
750 GCGC
Cfr10I 59 ACCGGC [GA]^CCGG[CT]
100 ACCGGC
EcoT14I 364 CCATGG C^C[AT][AT]GG
785 CCAAGG
HgaI 263 GCGTC GACGC(5/10)
488 GCGTC
HhaI 123 GCGC GCG^C
750 GCGC
HinP1I 123 GCGC G^CGC
750 GCGC
PvuII 139 CAGCTG CAG^CTG
171 CAGCTG
SmaI 374 CCCGGG CCC^GGG
461 CCCGGG
StuI 548 AGGCCT AGG^CCT
668 AGGCCT
StyI 364 CCATGG C^C[AT][AT]GG
785 CCAAGG
XmaCI 374 CCCGGG C^CCGGG
461 CCCGGG
XmaI 374 CCCGGG C^CCGGG
461 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BsmFI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI