(Circular) (Six-base) MAPSORT of: pFN21AE4308.seq Check: 9106 from: 1 to: 5659 pFN21AE4308 5659 bp With 182 enzymes: SgfI * March 28, 2012 14:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2955 3002 1937 Size: 1018 47 4594 Fragments arranged by size: 4594 1018 47 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3722 5106 5374 1632 Size: 2090 1384 268 1917 Fragments arranged by size: 2090 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2074 3402 4974 2074 Size: 1328 1572 2759 Fragments arranged by size: 2759 1572 1328 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2540 2968 3194 3346 5455 1493 Size: 1047 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 428 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2379 2466 2984 1870 Size: 73 436 87 518 4545 Fragments arranged by size: 4545 518 436 87 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2127 2980 4435 618 Size: 325 217 627 240 100 853 1455 1842 Fragments arranged by size: 1842 1455 853 627 325 240 217 100 BanII G_rGCy'C Cuts at: 1391 2978 1391 Size: 1587 4072 BbeI G_GCGC'C Cuts at: 2131 2131 Size: 5659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 928 Size: 971 190 4498 Fragments arranged by size: 4498 971 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2589 3551 4761 2589 Size: 962 1210 3487 Fragments arranged by size: 3487 1210 962 BclI T'GATC_A Cuts at: 1548 2478 1548 Size: 930 4729 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2681 2705 4270 Size: 107 122 71 631 605 1009 24 1565 Cuts at: 4270 136 Size: 1525 Fragments arranged by size: 1565 1525 1009 631 605 122 107 71 24 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2686 3720 4876 1790 Size: 896 1034 1156 2573 Fragments arranged by size: 2573 1156 1034 896 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2669 2678 2828 4319 1239 Size: 1331 99 9 150 1491 2579 Fragments arranged by size: 2579 1491 1331 150 99 9 Bpu10I CC'TnA_GC Cuts at: 2057 2057 Size: 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 2483 5653 2483 Size: 3170 2489 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2128 3466 3848 4483 Size: 53 83 186 936 595 1338 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1338 936 635 595 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 1178 Size: 30 165 30 1116 30 4288 Fragments arranged by size: 4288 1116 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2212 1186 Size: 1026 4633 BseYI C'CCAG_C Cuts at: 2023 2679 4862 5358 2023 Size: 656 2183 496 2324 Fragments arranged by size: 2324 2183 656 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5086 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2922 3720 3805 4876 2922 Size: 798 85 1071 3705 Fragments arranged by size: 3705 1071 798 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2686 2922 2978 3720 3805 4876 1391 Size: 399 896 236 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 896 742 399 236 85 56 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 2760 3548 5278 2760 Size: 788 1730 3141 Fragments arranged by size: 3141 1730 788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2901 3014 844 Size: 1370 687 113 3489 Fragments arranged by size: 3489 1370 687 113 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2092 4154 4328 65 Size: 1183 844 2062 174 1396 Fragments arranged by size: 2062 1396 1183 844 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 2105 4303 4490 1700 Size: 110 254 41 2198 187 2869 Fragments arranged by size: 2869 2198 254 187 110 41 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 794 Size: 1280 4379 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2431 1184 Size: 1247 4412 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2290 2497 2908 2245 Size: 45 207 411 4996 Fragments arranged by size: 4996 411 207 45 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2570 2570 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2369 513 Size: 552 1304 3803 Fragments arranged by size: 3803 1304 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2049 2138 3175 3969 3989 2049 Size: 89 1037 794 20 3719 Fragments arranged by size: 3719 1037 794 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 3106 3995 Size: 54 1425 50 275 18 342 934 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 934 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 3589 1353 Size: 669 1567 3423 Fragments arranged by size: 3423 1567 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2570 2669 2678 2828 3722 4319 5106 Size: 393 938 99 9 150 894 597 787 Cuts at: 5106 5374 1239 Size: 268 1524 Fragments arranged by size: 1524 938 894 787 597 393 268 150 99 9 EcoNI CCTnn'n_nnAGG Cuts at: 2789 2789 Size: 5659 EcoO109I rG'GnC_Cy Cuts at: 2203 2420 2721 2929 2203 Size: 217 301 208 4933 Fragments arranged by size: 4933 301 217 208 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 EcoRV GAT'ATC Cuts at: 2251 2251 Size: 5659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2333 2333 Size: 5659 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2131 4806 1678 Size: 453 2675 2531 Fragments arranged by size: 2675 2531 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2592 2624 4300 4332 4374 Size: 32 135 32 1020 32 1676 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1020 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KasI G'GCGC_C Cuts at: 2127 2127 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 4773 4957 1305 Size: 921 2547 184 2007 Fragments arranged by size: 2547 2007 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 10 Size: 54 1425 343 342 3495 Fragments arranged by size: 3495 1425 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 3976 4135 518 Size: 648 2451 359 159 2042 Fragments arranged by size: 2451 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2178 2212 2395 3075 3752 4900 5145 Size: 540 32 34 183 680 677 1148 245 Cuts at: 5145 1606 Size: 2120 Fragments arranged by size: 2120 1148 680 677 540 245 183 34 32 NarI GG'CG_CC Cuts at: 2128 2128 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 2369 513 Size: 552 1304 3803 Fragments arranged by size: 3803 1304 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2816 3017 4562 1132 Size: 97 1587 201 1545 2229 Fragments arranged by size: 2229 1587 1545 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2370 1108 Size: 1262 4397 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2420 2929 2203 Size: 217 509 4933 Fragments arranged by size: 4933 509 217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2145 2211 2460 2673 3011 830 Size: 1315 66 249 213 338 3478 Fragments arranged by size: 3478 1315 338 249 213 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2146 2178 2146 Size: 32 5627 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SanDI GG'GwC_CC Cuts at: 2420 2420 Size: 5659 SbfI CC_TGCA'GG Cuts at: 2673 3011 2673 Size: 338 5321 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2141 2207 2456 2669 2845 3007 4142 Size: 219 1096 66 249 213 176 162 1135 Cuts at: 4142 4823 5014 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1096 681 249 219 213 191 176 162 66 SfoI GGC'GCC Cuts at: 2129 2129 Size: 5659 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2381 2468 2986 1872 Size: 509 87 518 4545 Fragments arranged by size: 4545 518 509 87 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 2816 3017 1132 Size: 1684 201 3774 Fragments arranged by size: 3774 1684 201 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2555 2675 1517 Size: 1038 120 4501 Fragments arranged by size: 4501 1038 120 StyI C'CwwG_G Cuts at: 513 1065 2369 2790 3098 513 Size: 552 1304 421 308 3074 Fragments arranged by size: 3074 1304 552 421 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2015 2278 3686 3703 3856 4041 4486 Size: 1255 435 263 1408 17 153 185 445 Cuts at: 4486 325 Size: 1498 Fragments arranged by size: 1498 1408 1255 445 435 263 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 5619 5651 2404 Size: 32 3183 32 2412 Fragments arranged by size: 3183 2412 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2545 2545 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2379 2466 2984 1870 Size: 509 87 518 4545 Fragments arranged by size: 4545 518 509 87 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI