(Linear) (Six-base) MAPSORT of: KIEE4308.seq Check: 2999 from: 1 to: 954 KIEE4308.orf With 182 enzymes: SgfI * March 22, 2012 17:41 .. AccI GT'mk_AC Cuts at: 0 950 954 Size: 950 4 AlwNI CAG_nnn'CTG Cuts at: 0 69 954 Size: 69 885 ApoI r'AATT_y Cuts at: 0 535 954 Size: 535 419 AvaI C'yCGr_G Cuts at: 0 374 461 954 Size: 374 87 493 Fragments arranged by size: 493 374 87 BanI G'GyrC_C Cuts at: 0 22 122 954 Size: 22 100 832 Fragments arranged by size: 832 100 22 BbeI G_GCGC'C Cuts at: 0 126 954 Size: 126 828 BbsI GAAGACnn'nnnn_ Cuts at: 0 84 954 Size: 84 870 BciVI GTATCCnnnnn_n' Cuts at: 0 584 954 Size: 584 370 BclI T'GATC_A Cuts at: 0 473 954 Size: 473 481 BglI GCCn_nnn'nGGC Cuts at: 0 676 700 954 Size: 676 24 254 Fragments arranged by size: 676 254 24 Bme1580I G_kGCm'C Cuts at: 0 681 954 Size: 681 273 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 954 Size: 570 384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 664 673 823 954 Size: 565 99 9 150 131 Fragments arranged by size: 565 150 131 99 9 Bpu10I CC'TnA_GC Cuts at: 0 52 954 Size: 52 902 BsaBI GATnn'nnATC Cuts at: 0 478 954 Size: 478 476 BsaHI Gr'CG_yC Cuts at: 0 123 954 Size: 123 831 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 514 544 954 Size: 514 30 410 Fragments arranged by size: 514 410 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 207 954 Size: 207 747 BseYI C'CCAG_C Cuts at: 0 18 674 954 Size: 18 656 280 Fragments arranged by size: 656 280 18 BsiHKAI G_wGCw'C Cuts at: 0 917 954 Size: 917 37 Bsp1286I G_dGCh'C Cuts at: 0 681 917 954 Size: 681 236 37 Fragments arranged by size: 681 236 37 BspHI T'CATG_A Cuts at: 0 755 954 Size: 755 199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 209 896 954 Size: 209 687 58 Fragments arranged by size: 687 209 58 BsrDI GCAATG_nn' Cuts at: 0 87 954 Size: 87 867 BsrFI r'CCGG_y Cuts at: 0 59 100 954 Size: 59 41 854 Fragments arranged by size: 854 59 41 BstAPI GCAn_nnn'nTGC Cuts at: 0 69 954 Size: 69 885 BstEII G'GTnAC_C Cuts at: 0 426 954 Size: 426 528 BstXI CCAn_nnnn'nTGG Cuts at: 0 240 285 492 903 954 Size: 240 45 207 411 51 Fragments arranged by size: 411 240 207 51 45 Bsu36I CC'TnA_GG Cuts at: 0 565 954 Size: 565 389 BtgI C'CryG_G Cuts at: 0 364 954 Size: 364 590 BtsI GCAGTG_nn' Cuts at: 0 44 133 954 Size: 44 89 821 Fragments arranged by size: 821 89 44 EaeI y'GGCC_r Cuts at: 0 167 954 Size: 167 787 EarI CTCTTCn'nnn_ Cuts at: 0 17 954 Size: 17 937 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 664 673 823 954 Size: 565 99 9 150 131 Fragments arranged by size: 565 150 131 99 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 784 954 Size: 784 170 EcoO109I rG'GnC_Cy Cuts at: 0 198 415 716 924 954 Size: 198 217 301 208 30 Fragments arranged by size: 301 217 208 198 30 EcoRV GAT'ATC Cuts at: 0 246 954 Size: 246 708 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 328 954 Size: 328 626 HaeII r_GCGC'y Cuts at: 0 126 954 Size: 126 828 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 587 619 954 Size: 587 32 335 Fragments arranged by size: 587 335 32 KasI G'GCGC_C Cuts at: 0 122 954 Size: 122 832 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 221 954 Size: 221 733 MscI TGG'CCA Cuts at: 0 169 954 Size: 169 785 MspA1I CmG'CkG Cuts at: 0 141 173 207 390 954 Size: 141 32 34 183 564 Fragments arranged by size: 564 183 141 34 32 NarI GG'CG_CC Cuts at: 0 123 954 Size: 123 831 NcoI C'CATG_G Cuts at: 0 364 954 Size: 364 590 NspI r_CATG'y Cuts at: 0 811 954 Size: 811 143 PflMI CCAn_nnn'nTGG Cuts at: 0 365 954 Size: 365 589 PpuMI rG'GwC_Cy Cuts at: 0 198 415 924 954 Size: 198 217 509 30 Fragments arranged by size: 509 217 198 30 PstI C_TGCA'G Cuts at: 0 140 206 455 668 954 Size: 140 66 249 213 286 Fragments arranged by size: 286 249 213 140 66 PvuII CAG'CTG Cuts at: 0 141 173 954 Size: 141 32 781 Fragments arranged by size: 781 141 32 SanDI GG'GwC_CC Cuts at: 0 415 954 Size: 415 539 SbfI CC_TGCA'GG Cuts at: 0 668 954 Size: 668 286 SfcI C'TryA_G Cuts at: 0 136 202 451 664 840 954 Size: 136 66 249 213 176 114 Fragments arranged by size: 249 213 176 136 114 66 SfoI GGC'GCC Cuts at: 0 124 954 Size: 124 830 SmaI CCC'GGG Cuts at: 0 376 463 954 Size: 376 87 491 Fragments arranged by size: 491 376 87 SphI G_CATG'C Cuts at: 0 811 954 Size: 811 143 StuI AGG'CCT Cuts at: 0 550 670 954 Size: 550 120 284 Fragments arranged by size: 550 284 120 StyI C'CwwG_G Cuts at: 0 364 785 954 Size: 364 421 169 Fragments arranged by size: 421 364 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 10 273 954 Size: 10 263 681 Fragments arranged by size: 681 263 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 399 431 954 Size: 399 32 523 Fragments arranged by size: 523 399 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 540 954 Size: 540 414 XmaI C'CCGG_G Cuts at: 0 374 461 954 Size: 374 87 493 Fragments arranged by size: 493 374 87 Enzymes that do cut: AccI AlwNI ApoI AvaI BanI BbeI BbsI BciVI BclI BglI Bme1580I BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MscI MspA1I NarI NcoI NspI PflMI PpuMI PstI PvuII SanDI SbfI SfcI SfoI SmaI SphI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI