Restriction map for
pFN21AE5461
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AvaI AvaII AviII BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI BmyI Bpu1102I BpuAI
BsaAI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspI SwaI TaqI TspRI
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 182 TCCGGA T^CCGGA
AlwNI 408 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 772 ATTAAT AT^TAAT
AsnI 772 ATTAAT AT^TAAT
Asp700I 849 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 901 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 111 CCTAGG C^CTAGG
BalI 365 TGGCCA TGG^CCA
BamHI 674 GGATCC G^GATCC
BlnI 111 CCTAGG C^CTAGG
BpmI 157 CTCCAG CTGGAG(16/14)
BsaWI 182 TCCGGA [AT]^CCGG[AT]
BseAI 182 TCCGGA T^CCGGA
BsmAI 502 GTCTC GTCTC(1/5)
Bsp1407I 767 TGTACA T^GTACA
BspEI 182 TCCGGA T^CCGGA
BsrGI 767 TGTACA T^GTACA
BsrI 36 ACTGG ACTGG(1/-1)
BssSI 544 CTCGTG CACGAG(-5/-1)
BstAPI 407 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 674 GGATCC [GA]^GATC[CT]
EcoT14I 111 CCTAGG C^C[AT][AT]GG
HapII 183 CCGG C^CGG
HindIII 890 AAGCTT A^AGCTT
HpaII 183 CCGG C^CGG
MboII 176 TCTTC GAAGA(8/7)
MflI 674 GGATCC [GA]^GATC[CT]
MluNI 365 TGGCCA TGG^CCA
MroI 182 TCCGGA T^CCGGA
MscI 365 TGGCCA TGG^CCA
MspA1I 753 CAGCTG C[AC]G^C[GT]G
MspI 183 CCGG C^CGG
NlaIV 674 GGATCC GG[ACGT]^[ACGT]CC
PflFI 901 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 772 ATTAAT AT^TAAT
PvuII 753 CAGCTG CAG^CTG
SpeI 423 ACTAGT A^CTAGT
SspBI 767 TGTACA T^GTACA
StuI 701 AGGCCT AGG^CCT
StyI 111 CCTAGG C^C[AT][AT]GG
Tth111I 901 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 674 GGATCC [GA]^GATC[CT]
XmnI 849 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 186 GAATTT [GA]^AATT[CT]
203 AAATTT
AfaI 768 GTAC GT^AC
820 GTAC
AlwI 675 GATCC GGATC(4/5)
674 GGATC
ApoI 186 GAATTT [GA]^AATT[CT]
203 AAATTT
BsaJI 111 CCTAGG C^C[ACGT][ACGT]GG
485 CCCAGG
BsiYI 437 CCTGGTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
686 CCCAGCAAAGG
BslI 437 CCTGGTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
686 CCCAGCAAAGG
Cfr13I 39 GGGCC G^G[ACGT]CC
538 GGGCC
DpnII 355 GATC ^GATC
675 GATC
EaeI 365 TGGCCA [CT]^GGCC[GA]
599 TGGCCG
MboI 355 GATC ^GATC
675 GATC
MseI 773 TTAA T^TAA
868 TTAA
MwoI 272 GCTTTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCAGAGTCTGC
NdeII 355 GATC ^GATC
675 GATC
NspI 98 ACATGC [GA]CATG^[CT]
758 GCATGT
RsaI 768 GTAC GT^AC
820 GTAC
Sau3AI 355 GATC ^GATC
675 GATC
Sau96I 39 GGGCC G^G[ACGT]CC
538 GGGCC
Tru9I 773 TTAA T^TAA
868 TTAA
Tsp509I 187 AATT ^AATT
204 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BfaI BsaBI BssKI
BstF5I BstNI Cac8I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MamI MnlI
MslI MvaI NlaIII PleI ScrFI
TfiI TseI Tsp45I