################################## # NO CUT ENZYMES ( 200 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Asp700I 25 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 820 GTGCTC G[AT]GC[AT]^C BbvI 340 GCTGC GCAGC(8/12) BfaI 337 CTAG C^TAG BlpI 685 GCTCAGC GC^T[ACGT]AGC BmyI 820 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 685 GCTCAGC GC^T[ACGT]AGC BsaJI 254 CCAGGG C^C[ACGT][ACGT]GG BsiHKAI 820 GTGCTC G[AT]GC[AT]^C BsmAI 736 GTCTC GTCTC(1/5) BsmFI 39 GGGAC GGGAC(10/14) BsmI 224 GCATTC GAATGC(1/-1) Bsp1286I 820 GTGCTC G[AGT]GC[ACT]^C BspHI 398 TCATGA T^CATGA BspMI 712 ACCTGC ACCTGC(4/8) BsrDI 129 CATTGC GCAATG(2/0) BssKI 254 CCAGG ^CC[ACGT]GG BstNI 254 CCAGG CC^[AT]GG Cac8I 220 GCTTGC GC[ACGT]^[ACGT]GC CelII 685 GCTCAGC GC^T[ACGT]AGC EarI 717 CTCTTC CTCTTC(1/4) EcoRII 254 CCAGG ^CC[AT]GG Fnu4HI 340 GCTGC GC^[ACGT]GC HphI 242 TCACC GGTGA(8/7) ItaI 340 GCTGC GC^[ACGT]GC Ksp632I 717 CTCTTC CTCTTC(1/4) MaeI 337 CTAG C^TAG MvaI 254 CCAGG CC^[AT]GG MwoI 892 GCCTTCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 162 GACTC GAGTC(4/5) RcaI 398 TCATGA T^CATGA SapI 716 GCTCTTC GCTCTTC(1/4) ScrFI 254 CCAGG CC^[ACGT]GG SmlI 157 CTCAAG C^T[CT][GA]AG TseI 340 GCTGC G^C[AT]GC TspRI 470 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 25 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### BclI 455 TGATCA T^GATCA 516 TGATCA BsrI 445 ACTGG ACTGG(1/-1) 576 ACTGG FbaI 455 TGATCA T^GATCA 516 TGATCA Tsp45I 530 GTGAC ^GT[GC]AC 619 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AcsI AluI ApoI DdeI DpnII HinfI MaeIII MboI MboII MnlI MseI NdeII NlaIII Sau3AI TfiI Tru9I Tsp509I