################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 659 TCCGGA T^CCGGA AciI 357 CCGC CCGC(-3/-1) AflIII 98 ACATGT A^C[GA][CT]GT AlwI 148 GATCC GGATC(4/5) AlwNI 750 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 906 GGTCC G^G[AT]CC BalI 365 TGGCCA TGG^CCA BfaI 577 CTAG C^TAG BlpI 289 GCTCAGC GC^T[ACGT]AGC Bpu1102I 289 GCTCAGC GC^T[ACGT]AGC BsaWI 659 TCCGGA [AT]^CCGG[AT] BseAI 659 TCCGGA T^CCGGA BsmAI 736 GTCTC GTCTC(1/5) BsmI 224 GCATTC GAATGC(1/-1) Bsp1407I 767 TGTACA T^GTACA BspEI 659 TCCGGA T^CCGGA BspHI 398 TCATGA T^CATGA BspLU11I 98 ACATGT A^CATGT BspMI 63 GCAGGT ACCTGC(4/8) BsrGI 767 TGTACA T^GTACA BstEII 618 GGTCACC G^GT[ACGT]ACC BstF5I 657 CATCC GGATG(2/0) BstPI 618 GGTCACC G^GT[ACGT]ACC BstYI 147 AGATCC [GA]^GATC[CT] CelII 289 GCTCAGC GC^T[ACGT]AGC Cfr13I 906 GGTCC G^G[ACGT]CC DraI 927 TTTAAA TTT^AAA EaeI 365 TGGCCA [CT]^GGCC[GA] EcoO65I 618 GGTCACC G^GT[ACGT]ACC FokI 657 CATCC GGATG(9/13) HaeIII 366 GGCC GG^CC HapII 660 CCGG C^CGG HpaII 660 CCGG C^CGG MaeI 577 CTAG C^TAG MaeII 214 ACGT A^CGT MflI 147 AGATCC [GA]^GATC[CT] MluNI 365 TGGCCA TGG^CCA MroI 659 TCCGGA T^CCGGA MscI 365 TGGCCA TGG^CCA MspA1I 357 CCGCTG C[AC]G^C[GT]G MspI 660 CCGG C^CGG NdeI 724 CATATG CA^TATG NspI 98 ACATGT [GA]CATG^[CT] RcaI 398 TCATGA T^CATGA Sau96I 906 GGTCC G^G[ACGT]CC SspBI 767 TGTACA T^GTACA XbaI 576 TCTAGA T^CTAGA XhoII 147 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 263 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 849 GAATCCCTTC AspHI 402 GAGCAC G[AT]GC[AT]^C 820 GTGCTC BmyI 402 GAGCAC G[AGT]GC[ACT]^C 820 GTGCTC BsaBI 123 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 148 GATCCCCATC BseRI 717 CTCCTC GAGGAG(10/8) 839 CTCCTC BsiHKAI 402 GAGCAC G[AT]GC[AT]^C 820 GTGCTC BsiYI 357 CCGCTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 785 CCAGAGAAAGG BslI 357 CCGCTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 785 CCAGAGAAAGG Bsp1286I 402 GAGCAC G[AGT]GC[ACT]^C 820 GTGCTC BsrI 112 ACTGG ACTGG(1/-1) 469 CCAGT BssKI 198 CCTGG ^CC[ACGT]GG 437 CCTGG BstNI 198 CCTGG CC^[AT]GG 437 CCTGG Cac8I 441 GCTTGC GC[ACGT]^[ACGT]GC 888 GCAAGC EarI 380 CTCTTC CTCTTC(1/4) 534 CTCTTC EcoRII 198 CCTGG ^CC[AT]GG 437 CCTGG Ksp632I 380 CTCTTC CTCTTC(1/4) 534 CTCTTC MamI 123 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 148 GATCCCCATC MslI 243 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 405 CACCAGAGTG MvaI 198 CCTGG CC^[AT]GG 437 CCTGG MwoI 477 GCTTCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 879 GCAAGTGAAGC NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC 837 GGCTCC ScrFI 198 CCTGG CC^[ACGT]GG 437 CCTGG TfiI 233 GATTC G^A[AT]TC 849 GAATC XmnI 263 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 849 GAATCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BclI DdeI DpnII FbaI Fnu4HI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII PleI PstI RsaI Sau3AI SfcI Tru9I TseI Tsp45I Tsp509I TspRI